Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF781 and GFF782 are separated by 5 nucleotides GFF782 and GFF783 are separated by 101 nucleotides GFF783 and GFF784 are separated by 11 nucleotides
GFF781 - hypothetical protein, at 62,685 to 64,532
GFF781
GFF782 - Alpha-ketoglutarate-dependent dioxygenase AlkB (EC 1.14.11.33), at 64,538 to 65,245
GFF782
GFF783 - no description, at 65,347 to 66,069
GFF783
GFF784 - KEGG: RNA polymerase sigma-70 factor, ECF subfamily, at 66,081 to 66,641
GFF784
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.559 kb on + strand, within GFF781 at 63.559 kb on + strand, within GFF781 at 63.634 kb on + strand, within GFF781 at 63.640 kb on + strand, within GFF781 at 63.640 kb on + strand, within GFF781 at 63.640 kb on + strand, within GFF781 at 63.641 kb on - strand, within GFF781 at 63.641 kb on - strand, within GFF781 at 63.641 kb on - strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.700 kb on + strand, within GFF781 at 63.701 kb on - strand, within GFF781 at 63.701 kb on - strand, within GFF781 at 63.701 kb on - strand, within GFF781 at 63.902 kb on - strand, within GFF781 at 63.985 kb on + strand, within GFF781 at 64.193 kb on - strand, within GFF781 at 64.193 kb on - strand, within GFF781 at 64.193 kb on - strand, within GFF781 at 64.282 kb on + strand, within GFF781 at 64.282 kb on + strand, within GFF781 at 64.283 kb on - strand, within GFF781 at 64.283 kb on - strand, within GFF781 at 64.465 kb on + strand at 64.465 kb on + strand at 64.466 kb on - strand at 64.537 kb on - strand at 64.682 kb on + strand, within GFF782 at 64.683 kb on - strand, within GFF782 at 64.764 kb on + strand, within GFF782 at 64.765 kb on - strand, within GFF782 at 64.815 kb on + strand, within GFF782 at 64.816 kb on - strand, within GFF782 at 65.268 kb on + strand at 65.268 kb on + strand at 65.268 kb on + strand at 65.289 kb on + strand at 65.289 kb on + strand at 65.292 kb on + strand at 65.292 kb on + strand at 65.293 kb on - strand at 65.293 kb on - strand at 65.656 kb on + strand, within GFF783 at 66.132 kb on + strand at 66.132 kb on + strand at 66.132 kb on + strand at 66.132 kb on + strand at 66.133 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 63,559 + GFF781 0.47 +0.0 63,559 + GFF781 0.47 -1.9 63,634 + GFF781 0.51 -0.7 63,640 + GFF781 0.52 -0.6 63,640 + GFF781 0.52 +0.5 63,640 + GFF781 0.52 -0.9 63,641 - GFF781 0.52 -1.0 63,641 - GFF781 0.52 +0.7 63,641 - GFF781 0.52 -0.6 63,700 + GFF781 0.55 -0.6 63,700 + GFF781 0.55 -0.9 63,700 + GFF781 0.55 -1.4 63,700 + GFF781 0.55 -1.1 63,700 + GFF781 0.55 -1.7 63,701 - GFF781 0.55 -1.9 63,701 - GFF781 0.55 -0.4 63,701 - GFF781 0.55 -0.5 63,902 - GFF781 0.66 +0.0 63,985 + GFF781 0.70 +0.5 64,193 - GFF781 0.82 +1.4 64,193 - GFF781 0.82 -0.9 64,193 - GFF781 0.82 -0.4 64,282 + GFF781 0.86 -0.7 64,282 + GFF781 0.86 +1.4 64,283 - GFF781 0.86 +0.7 64,283 - GFF781 0.86 +0.1 64,465 + -1.0 64,465 + -1.0 64,466 - -1.1 64,537 - +0.1 64,682 + GFF782 0.20 -0.1 64,683 - GFF782 0.20 -1.1 64,764 + GFF782 0.32 +0.5 64,765 - GFF782 0.32 -0.3 64,815 + GFF782 0.39 +2.3 64,816 - GFF782 0.39 +1.0 65,268 + -1.3 65,268 + -0.4 65,268 + -0.3 65,289 + +1.2 65,289 + +0.1 65,292 + -1.1 65,292 + +0.7 65,293 - -1.4 65,293 - -0.0 65,656 + GFF783 0.43 -0.6 66,132 + -0.5 66,132 + -0.8 66,132 + +0.6 66,132 + -0.2 66,133 - -0.6
Or see this region's nucleotide sequence