Strain Fitness in Variovorax sp. SCN45 around GFF759

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF757 and GFF758 are separated by 9 nucleotidesGFF758 and GFF759 are separated by 13 nucleotidesGFF759 and GFF760 are separated by 22 nucleotides GFF757 - hypothetical protein, at 20,708 to 21,535 GFF757 GFF758 - 2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55), at 21,545 to 22,390 GFF758 GFF759 - FIG01121861: hypothetical protein, at 22,404 to 23,597 GFF759 GFF760 - hypothetical protein, at 23,620 to 24,579 GFF760 Position (kb) 22 23 24Strain fitness (log2 ratio) -1 0 1 2at 21.550 kb on + strandat 21.550 kb on + strandat 21.550 kb on + strandat 21.551 kb on - strandat 22.405 kb on + strandat 22.405 kb on + strandat 22.406 kb on - strandat 22.543 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.564 kb on + strand, within GFF759at 22.565 kb on - strand, within GFF759at 22.565 kb on - strand, within GFF759at 22.565 kb on - strand, within GFF759at 22.565 kb on - strand, within GFF759at 22.565 kb on - strand, within GFF759at 22.565 kb on - strand, within GFF759at 22.627 kb on + strand, within GFF759at 22.627 kb on + strand, within GFF759at 22.627 kb on + strand, within GFF759at 22.627 kb on + strand, within GFF759at 22.627 kb on + strand, within GFF759at 22.628 kb on - strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.756 kb on + strand, within GFF759at 22.757 kb on - strand, within GFF759at 22.757 kb on - strand, within GFF759at 22.757 kb on - strand, within GFF759at 22.757 kb on - strand, within GFF759at 22.757 kb on - strand, within GFF759at 22.757 kb on - strand, within GFF759at 23.320 kb on + strand, within GFF759at 23.320 kb on + strand, within GFF759at 23.320 kb on + strand, within GFF759at 23.321 kb on - strand, within GFF759at 23.321 kb on - strand, within GFF759at 23.321 kb on - strand, within GFF759at 23.354 kb on - strand, within GFF759at 23.354 kb on - strand, within GFF759at 23.354 kb on - strand, within GFF759at 23.354 kb on - strand, within GFF759at 23.354 kb on - strand, within GFF759at 23.354 kb on - strand, within GFF759at 23.359 kb on + strand, within GFF759at 23.645 kb on + strandat 24.543 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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21,550 + -0.5
21,550 + -0.3
21,550 + -0.1
21,551 - -0.2
22,405 + -0.4
22,405 + -0.6
22,406 - -0.6
22,543 + GFF759 0.12 +0.3
22,564 + GFF759 0.13 +0.7
22,564 + GFF759 0.13 -0.4
22,564 + GFF759 0.13 -0.9
22,564 + GFF759 0.13 +0.3
22,564 + GFF759 0.13 -0.7
22,564 + GFF759 0.13 +0.9
22,564 + GFF759 0.13 +0.0
22,564 + GFF759 0.13 -0.3
22,564 + GFF759 0.13 -0.7
22,564 + GFF759 0.13 -1.1
22,565 - GFF759 0.13 -0.4
22,565 - GFF759 0.13 -0.1
22,565 - GFF759 0.13 -0.4
22,565 - GFF759 0.13 +1.6
22,565 - GFF759 0.13 -0.5
22,565 - GFF759 0.13 +0.1
22,627 + GFF759 0.19 -0.2
22,627 + GFF759 0.19 +1.4
22,627 + GFF759 0.19 -1.1
22,627 + GFF759 0.19 -0.9
22,627 + GFF759 0.19 -0.0
22,628 - GFF759 0.19 +0.3
22,756 + GFF759 0.29 +0.3
22,756 + GFF759 0.29 -0.4
22,756 + GFF759 0.29 -0.9
22,756 + GFF759 0.29 -1.6
22,756 + GFF759 0.29 +0.2
22,756 + GFF759 0.29 +0.3
22,756 + GFF759 0.29 -0.5
22,756 + GFF759 0.29 -1.4
22,757 - GFF759 0.30 +0.4
22,757 - GFF759 0.30 +0.4
22,757 - GFF759 0.30 -0.1
22,757 - GFF759 0.30 -0.2
22,757 - GFF759 0.30 +0.1
22,757 - GFF759 0.30 -0.7
23,320 + GFF759 0.77 -0.2
23,320 + GFF759 0.77 +0.1
23,320 + GFF759 0.77 +1.9
23,321 - GFF759 0.77 -0.2
23,321 - GFF759 0.77 +0.1
23,321 - GFF759 0.77 -0.6
23,354 - GFF759 0.80 +0.1
23,354 - GFF759 0.80 +0.3
23,354 - GFF759 0.80 +0.8
23,354 - GFF759 0.80 -0.2
23,354 - GFF759 0.80 +0.0
23,354 - GFF759 0.80 -0.3
23,359 + GFF759 0.80 +0.4
23,645 + +1.1
24,543 - +0.2

Or see this region's nucleotide sequence