Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7383 and GFF7384 are separated by 7 nucleotides GFF7384 and GFF7385 are separated by 72 nucleotides
GFF7383 - Esterase/lipase, at 911 to 1,903
GFF7383
GFF7384 - hypothetical protein, at 1,911 to 3,020
GFF7384
GFF7385 - hypothetical protein, at 3,093 to 4,460
GFF7385
Position (kb)
1
2
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 0.999 kb on + strand at 0.999 kb on + strand at 1.119 kb on - strand, within GFF7383 at 1.119 kb on - strand, within GFF7383 at 1.164 kb on - strand, within GFF7383 at 1.205 kb on + strand, within GFF7383 at 1.205 kb on + strand, within GFF7383 at 1.348 kb on + strand, within GFF7383 at 1.390 kb on + strand, within GFF7383 at 1.391 kb on - strand, within GFF7383 at 1.405 kb on + strand, within GFF7383 at 1.406 kb on - strand, within GFF7383 at 1.458 kb on + strand, within GFF7383 at 1.459 kb on - strand, within GFF7383 at 1.459 kb on - strand, within GFF7383 at 1.459 kb on - strand, within GFF7383 at 1.511 kb on + strand, within GFF7383 at 1.512 kb on - strand, within GFF7383 at 1.742 kb on + strand, within GFF7383 at 1.742 kb on + strand, within GFF7383 at 1.742 kb on + strand, within GFF7383 at 1.743 kb on - strand, within GFF7383 at 1.765 kb on - strand, within GFF7383 at 2.175 kb on + strand, within GFF7384 at 2.223 kb on + strand, within GFF7384 at 2.223 kb on + strand, within GFF7384 at 2.223 kb on + strand, within GFF7384 at 2.937 kb on + strand at 3.090 kb on + strand at 3.091 kb on - strand at 3.136 kb on + strand at 3.136 kb on + strand at 3.136 kb on + strand at 3.180 kb on + strand at 3.180 kb on + strand at 3.181 kb on - strand at 3.181 kb on - strand at 3.181 kb on - strand at 3.249 kb on + strand, within GFF7385 at 3.250 kb on - strand, within GFF7385 at 3.315 kb on + strand, within GFF7385 at 3.316 kb on - strand, within GFF7385 at 3.316 kb on - strand, within GFF7385 at 3.437 kb on - strand, within GFF7385 at 3.498 kb on + strand, within GFF7385 at 3.498 kb on + strand, within GFF7385 at 3.499 kb on - strand, within GFF7385 at 3.538 kb on - strand, within GFF7385 at 3.610 kb on - strand, within GFF7385 at 3.879 kb on - strand, within GFF7385 at 3.885 kb on - strand, within GFF7385 at 3.891 kb on + strand, within GFF7385 at 3.892 kb on - strand, within GFF7385
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 999 + -0.2 999 + +0.5 1,119 - GFF7383 0.21 +0.8 1,119 - GFF7383 0.21 +0.5 1,164 - GFF7383 0.25 -0.1 1,205 + GFF7383 0.30 -0.2 1,205 + GFF7383 0.30 +0.8 1,348 + GFF7383 0.44 -1.7 1,390 + GFF7383 0.48 -2.0 1,391 - GFF7383 0.48 -0.1 1,405 + GFF7383 0.50 -0.5 1,406 - GFF7383 0.50 +1.2 1,458 + GFF7383 0.55 +1.4 1,459 - GFF7383 0.55 -0.2 1,459 - GFF7383 0.55 +0.0 1,459 - GFF7383 0.55 -1.0 1,511 + GFF7383 0.60 +0.4 1,512 - GFF7383 0.61 -0.7 1,742 + GFF7383 0.84 -0.8 1,742 + GFF7383 0.84 +0.1 1,742 + GFF7383 0.84 -0.9 1,743 - GFF7383 0.84 +0.2 1,765 - GFF7383 0.86 -0.5 2,175 + GFF7384 0.24 -0.8 2,223 + GFF7384 0.28 -1.0 2,223 + GFF7384 0.28 -0.5 2,223 + GFF7384 0.28 +0.1 2,937 + -1.1 3,090 + -0.9 3,091 - +0.5 3,136 + +1.0 3,136 + +1.8 3,136 + -0.8 3,180 + -0.7 3,180 + -0.9 3,181 - +0.3 3,181 - -0.6 3,181 - -0.4 3,249 + GFF7385 0.11 -0.6 3,250 - GFF7385 0.11 +1.2 3,315 + GFF7385 0.16 -0.9 3,316 - GFF7385 0.16 -0.1 3,316 - GFF7385 0.16 +0.2 3,437 - GFF7385 0.25 -0.2 3,498 + GFF7385 0.30 +0.9 3,498 + GFF7385 0.30 -0.9 3,499 - GFF7385 0.30 -0.4 3,538 - GFF7385 0.33 +0.6 3,610 - GFF7385 0.38 -0.3 3,879 - GFF7385 0.57 +1.0 3,885 - GFF7385 0.58 -0.7 3,891 + GFF7385 0.58 -0.2 3,892 - GFF7385 0.58 -1.0
Or see this region's nucleotide sequence