Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7370 and GFF7371 are separated by 94 nucleotides GFF7371 and GFF7372 are separated by 1 nucleotides GFF7372 and GFF7373 are separated by 72 nucleotides
GFF7370 - PAS domain with LuxR-type DNA-binding domain, at 73 to 639
GFF7370
GFF7371 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 734 to 1,546
GFF7371
GFF7372 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 1,548 to 2,186
GFF7372
GFF7373 - CaiB/BaiF family protein, at 2,259 to 3,650
GFF7373
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1 at 0.122 kb on + strand at 0.122 kb on + strand at 0.123 kb on - strand at 0.123 kb on - strand at 0.123 kb on - strand at 0.417 kb on - strand, within GFF7370 at 0.417 kb on - strand, within GFF7370 at 0.485 kb on + strand, within GFF7370 at 0.485 kb on + strand, within GFF7370 at 0.660 kb on + strand at 0.661 kb on - strand at 0.663 kb on + strand at 0.664 kb on - strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.667 kb on + strand at 0.668 kb on - strand at 0.675 kb on + strand at 0.676 kb on - strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.679 kb on + strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.680 kb on - strand at 0.747 kb on - strand at 0.965 kb on + strand, within GFF7371 at 0.965 kb on + strand, within GFF7371 at 0.965 kb on + strand, within GFF7371 at 0.966 kb on - strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.998 kb on + strand, within GFF7371 at 0.999 kb on - strand, within GFF7371 at 0.999 kb on - strand, within GFF7371 at 0.999 kb on - strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.157 kb on + strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.158 kb on - strand, within GFF7371 at 1.361 kb on + strand, within GFF7371 at 1.361 kb on + strand, within GFF7371 at 1.361 kb on + strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.362 kb on - strand, within GFF7371 at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.608 kb on + strand at 1.609 kb on - strand at 1.609 kb on - strand at 1.609 kb on - strand at 1.609 kb on - strand at 1.634 kb on + strand, within GFF7372 at 1.634 kb on + strand, within GFF7372 at 1.634 kb on + strand, within GFF7372 at 1.635 kb on - strand, within GFF7372 at 1.635 kb on - strand, within GFF7372 at 2.184 kb on + strand at 2.185 kb on - strand at 2.524 kb on + strand, within GFF7373 at 2.525 kb on - strand, within GFF7373
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 122 + -2.3 122 + -0.2 123 - -0.7 123 - -0.4 123 - +0.3 417 - GFF7370 0.61 -1.1 417 - GFF7370 0.61 -0.5 485 + GFF7370 0.73 +0.1 485 + GFF7370 0.73 -0.8 660 + -0.1 661 - -0.7 663 + -0.7 664 - +1.0 667 + +0.5 667 + -0.1 667 + -0.2 667 + -0.6 667 + -2.0 668 - -1.0 675 + -0.3 676 - -0.5 679 + -0.9 679 + -0.1 679 + -0.2 679 + -0.4 679 + +0.1 680 - -0.1 680 - +0.5 680 - -1.5 680 - +0.3 680 - +0.1 680 - -0.3 747 - +0.2 965 + GFF7371 0.28 -0.2 965 + GFF7371 0.28 -0.6 965 + GFF7371 0.28 +0.8 966 - GFF7371 0.29 -0.3 998 + GFF7371 0.32 -0.2 998 + GFF7371 0.32 -0.3 998 + GFF7371 0.32 +0.5 998 + GFF7371 0.32 +0.9 999 - GFF7371 0.33 -0.5 999 - GFF7371 0.33 +0.1 999 - GFF7371 0.33 -0.2 1,157 + GFF7371 0.52 -0.3 1,157 + GFF7371 0.52 -0.7 1,157 + GFF7371 0.52 -1.1 1,157 + GFF7371 0.52 +0.5 1,158 - GFF7371 0.52 -0.5 1,158 - GFF7371 0.52 -1.0 1,158 - GFF7371 0.52 -1.5 1,158 - GFF7371 0.52 -0.1 1,158 - GFF7371 0.52 -1.0 1,158 - GFF7371 0.52 -1.1 1,158 - GFF7371 0.52 -0.4 1,361 + GFF7371 0.77 -0.5 1,361 + GFF7371 0.77 -0.4 1,361 + GFF7371 0.77 -0.5 1,362 - GFF7371 0.77 -0.3 1,362 - GFF7371 0.77 -0.1 1,362 - GFF7371 0.77 -1.0 1,362 - GFF7371 0.77 -0.2 1,362 - GFF7371 0.77 -0.1 1,362 - GFF7371 0.77 -0.3 1,362 - GFF7371 0.77 -0.4 1,362 - GFF7371 0.77 -0.3 1,608 + +0.4 1,608 + -0.5 1,608 + +0.3 1,608 + -0.1 1,608 + -0.8 1,608 + -0.7 1,608 + +0.3 1,608 + +0.0 1,609 - -0.2 1,609 - -0.5 1,609 - -1.5 1,609 - +0.7 1,634 + GFF7372 0.13 -0.3 1,634 + GFF7372 0.13 +1.2 1,634 + GFF7372 0.13 -0.2 1,635 - GFF7372 0.14 -0.2 1,635 - GFF7372 0.14 -1.6 2,184 + +1.6 2,185 - -0.6 2,524 + GFF7373 0.19 -0.3 2,525 - GFF7373 0.19 +0.5
Or see this region's nucleotide sequence