Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7273 and GFF7274 overlap by 4 nucleotides GFF7274 and GFF7275 are separated by 93 nucleotides GFF7275 and GFF7276 are separated by 128 nucleotides
GFF7273 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 22,624 to 23,481
GFF7273
GFF7274 - hypothetical protein, at 23,478 to 24,575
GFF7274
GFF7275 - no description, at 24,669 to 24,794
GFF7275
GFF7276 - Thioesterase, at 24,923 to 25,354
GFF7276
Position (kb) 23
24
25 Strain fitness (log2 ratio) -1
0
1
2 at 22.659 kb on - strand at 22.939 kb on + strand, within GFF7273 at 22.940 kb on - strand, within GFF7273 at 23.080 kb on + strand, within GFF7273 at 23.081 kb on - strand, within GFF7273 at 23.081 kb on - strand, within GFF7273 at 23.081 kb on - strand, within GFF7273 at 23.266 kb on + strand, within GFF7273 at 23.285 kb on - strand, within GFF7273 at 23.296 kb on + strand, within GFF7273 at 23.457 kb on + strand at 23.873 kb on - strand, within GFF7274 at 24.223 kb on - strand, within GFF7274 at 24.410 kb on - strand, within GFF7274 at 24.436 kb on + strand, within GFF7274 at 24.437 kb on - strand, within GFF7274 at 24.438 kb on + strand, within GFF7274 at 24.438 kb on + strand, within GFF7274 at 24.439 kb on - strand, within GFF7274 at 24.680 kb on - strand at 24.821 kb on + strand at 24.880 kb on - strand at 24.880 kb on - strand at 24.911 kb on + strand at 24.912 kb on - strand at 25.020 kb on + strand, within GFF7276 at 25.443 kb on + strand at 25.443 kb on + strand at 25.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 22,659 - +0.2 22,939 + GFF7273 0.37 +0.4 22,940 - GFF7273 0.37 +1.9 23,080 + GFF7273 0.53 +0.1 23,081 - GFF7273 0.53 -0.8 23,081 - GFF7273 0.53 -0.5 23,081 - GFF7273 0.53 -0.5 23,266 + GFF7273 0.75 -1.1 23,285 - GFF7273 0.77 +0.1 23,296 + GFF7273 0.78 +0.1 23,457 + -0.3 23,873 - GFF7274 0.36 +0.2 24,223 - GFF7274 0.68 -1.7 24,410 - GFF7274 0.85 +0.6 24,436 + GFF7274 0.87 -0.8 24,437 - GFF7274 0.87 +0.3 24,438 + GFF7274 0.87 -0.8 24,438 + GFF7274 0.87 +0.7 24,439 - GFF7274 0.88 +0.1 24,680 - -0.3 24,821 + +0.2 24,880 - +1.2 24,880 - -0.6 24,911 + +1.2 24,912 - -0.9 25,020 + GFF7276 0.22 -0.3 25,443 + -0.2 25,443 + +0.3 25,444 - +0.3
Or see this region's nucleotide sequence