Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7214 and GFF7215 are separated by 170 nucleotides GFF7215 and GFF7216 are separated by 49 nucleotides GFF7216 and GFF7217 are separated by 38 nucleotides
GFF7214 - Peptidase M20D, amidohydrolase, at 32,670 to 33,980
GFF7214
GFF7215 - Transcriptional regulator, LysR family, at 34,151 to 35,035
GFF7215
GFF7216 - hypothetical protein, at 35,085 to 35,549
GFF7216
GFF7217 - hypothetical protein, at 35,588 to 36,508
GFF7217
Position (kb) 34
35
36 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 33.635 kb on - strand, within GFF7214 at 33.695 kb on + strand, within GFF7214 at 33.696 kb on - strand, within GFF7214 at 33.847 kb on + strand, within GFF7214 at 33.848 kb on - strand, within GFF7214 at 33.868 kb on + strand at 33.869 kb on - strand at 34.068 kb on + strand at 34.161 kb on + strand at 34.162 kb on - strand at 34.162 kb on - strand at 34.701 kb on + strand, within GFF7215 at 34.781 kb on + strand, within GFF7215 at 34.781 kb on + strand, within GFF7215 at 34.781 kb on + strand, within GFF7215 at 34.782 kb on - strand, within GFF7215 at 34.782 kb on - strand, within GFF7215 at 34.782 kb on - strand, within GFF7215 at 34.932 kb on + strand, within GFF7215 at 34.933 kb on - strand, within GFF7215 at 34.933 kb on - strand, within GFF7215 at 34.933 kb on - strand, within GFF7215 at 34.933 kb on - strand, within GFF7215 at 35.118 kb on + strand at 35.119 kb on - strand at 35.335 kb on + strand, within GFF7216 at 35.415 kb on + strand, within GFF7216 at 35.415 kb on + strand, within GFF7216 at 35.485 kb on - strand, within GFF7216 at 35.844 kb on - strand, within GFF7217 at 36.000 kb on + strand, within GFF7217
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 33,635 - GFF7214 0.74 +0.7 33,695 + GFF7214 0.78 +0.3 33,696 - GFF7214 0.78 -0.5 33,847 + GFF7214 0.90 +0.7 33,848 - GFF7214 0.90 -0.3 33,868 + -0.9 33,869 - +2.3 34,068 + -0.0 34,161 + +0.0 34,162 - +0.1 34,162 - +0.5 34,701 + GFF7215 0.62 +0.0 34,781 + GFF7215 0.71 -0.1 34,781 + GFF7215 0.71 +0.6 34,781 + GFF7215 0.71 -1.5 34,782 - GFF7215 0.71 -0.5 34,782 - GFF7215 0.71 +0.3 34,782 - GFF7215 0.71 -2.1 34,932 + GFF7215 0.88 +0.9 34,933 - GFF7215 0.88 -0.1 34,933 - GFF7215 0.88 -2.5 34,933 - GFF7215 0.88 +1.2 34,933 - GFF7215 0.88 +1.0 35,118 + +0.2 35,119 - -0.0 35,335 + GFF7216 0.54 +0.4 35,415 + GFF7216 0.71 +1.2 35,415 + GFF7216 0.71 -2.9 35,485 - GFF7216 0.86 -0.2 35,844 - GFF7217 0.28 -0.2 36,000 + GFF7217 0.45 +0.2
Or see this region's nucleotide sequence