Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7089 and GFF7090 are separated by 23 nucleotides GFF7090 and GFF7091 are separated by 8 nucleotides GFF7091 and GFF7092 overlap by 4 nucleotides GFF7092 and GFF7093 are separated by 524 nucleotides GFF7093 and GFF7094 are separated by 52 nucleotides GFF7094 and GFF7095 are separated by 54 nucleotides
GFF7089 - Putative short-chain dehydrogenase, at 11,961 to 12,749
GFF7089
GFF7090 - Small subunit naph/bph dioxygenase, at 12,773 to 13,366
GFF7090
GFF7091 - no description, at 13,375 to 13,554
GFF7091
GFF7092 - no description, at 13,551 to 13,703
GFF7092
GFF7093 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 14,228 to 14,380
GFF7093
GFF7094 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 14,433 to 14,618
GFF7094
GFF7095 - BUG/TctC family periplasmic protein, at 14,673 to 14,948
GFF7095
Position (kb) 13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 12.608 kb on - strand, within GFF7089 at 12.637 kb on + strand, within GFF7089 at 12.638 kb on - strand, within GFF7089 at 12.718 kb on - strand at 12.826 kb on + strand at 12.827 kb on - strand at 12.845 kb on + strand, within GFF7090 at 12.845 kb on + strand, within GFF7090 at 12.846 kb on - strand, within GFF7090 at 12.848 kb on + strand, within GFF7090 at 12.848 kb on + strand, within GFF7090 at 12.848 kb on + strand, within GFF7090 at 12.848 kb on + strand, within GFF7090 at 12.848 kb on + strand, within GFF7090 at 12.848 kb on + strand, within GFF7090 at 12.849 kb on - strand, within GFF7090 at 12.849 kb on - strand, within GFF7090 at 12.849 kb on - strand, within GFF7090 at 12.953 kb on + strand, within GFF7090 at 12.954 kb on - strand, within GFF7090 at 12.954 kb on - strand, within GFF7090 at 12.954 kb on - strand, within GFF7090 at 13.150 kb on + strand, within GFF7090 at 13.151 kb on - strand, within GFF7090 at 13.168 kb on + strand, within GFF7090 at 13.168 kb on + strand, within GFF7090 at 13.168 kb on + strand, within GFF7090 at 13.168 kb on + strand, within GFF7090 at 13.169 kb on - strand, within GFF7090 at 13.169 kb on - strand, within GFF7090 at 13.169 kb on - strand, within GFF7090 at 13.214 kb on + strand, within GFF7090 at 13.214 kb on + strand, within GFF7090 at 13.214 kb on + strand, within GFF7090 at 13.215 kb on - strand, within GFF7090 at 13.233 kb on - strand, within GFF7090 at 13.241 kb on + strand, within GFF7090 at 13.241 kb on + strand, within GFF7090 at 13.242 kb on - strand, within GFF7090 at 13.332 kb on + strand at 13.332 kb on + strand at 13.332 kb on + strand at 13.333 kb on - strand at 13.333 kb on - strand at 13.365 kb on - strand at 13.365 kb on - strand at 13.395 kb on + strand, within GFF7091 at 13.409 kb on + strand, within GFF7091 at 13.410 kb on - strand, within GFF7091 at 13.449 kb on - strand, within GFF7091 at 13.602 kb on + strand, within GFF7092 at 13.602 kb on + strand, within GFF7092 at 13.603 kb on - strand, within GFF7092 at 13.603 kb on - strand, within GFF7092 at 13.603 kb on - strand, within GFF7092 at 13.603 kb on - strand, within GFF7092 at 13.603 kb on - strand, within GFF7092 at 13.667 kb on + strand, within GFF7092 at 13.667 kb on + strand, within GFF7092 at 13.668 kb on - strand, within GFF7092 at 13.668 kb on - strand, within GFF7092 at 13.668 kb on - strand, within GFF7092 at 13.668 kb on - strand, within GFF7092 at 13.668 kb on - strand, within GFF7092 at 13.779 kb on + strand at 13.779 kb on + strand at 13.779 kb on + strand at 13.780 kb on - strand at 13.780 kb on - strand at 13.783 kb on + strand at 13.784 kb on - strand at 13.784 kb on - strand at 13.784 kb on - strand at 13.784 kb on - strand at 13.784 kb on - strand at 13.841 kb on + strand at 13.842 kb on - strand at 13.842 kb on - strand at 13.842 kb on - strand at 13.842 kb on - strand at 13.854 kb on - strand at 13.871 kb on + strand at 13.872 kb on - strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.895 kb on + strand at 13.896 kb on - strand at 13.896 kb on - strand at 13.896 kb on - strand at 13.949 kb on + strand at 13.950 kb on - strand at 13.950 kb on - strand at 13.950 kb on - strand at 13.950 kb on - strand at 13.990 kb on + strand at 13.990 kb on + strand at 13.990 kb on + strand at 13.990 kb on + strand at 13.990 kb on + strand at 13.991 kb on - strand at 14.003 kb on + strand at 14.003 kb on + strand at 14.004 kb on - strand at 14.004 kb on - strand at 14.167 kb on + strand at 14.167 kb on + strand at 14.168 kb on - strand at 14.168 kb on - strand at 14.168 kb on - strand at 14.168 kb on - strand at 14.168 kb on - strand at 14.168 kb on - strand at 14.168 kb on - strand at 14.250 kb on - strand, within GFF7093 at 14.383 kb on + strand at 14.383 kb on + strand at 14.383 kb on + strand at 14.383 kb on + strand at 14.384 kb on - strand at 14.384 kb on - strand at 14.412 kb on - strand at 14.517 kb on + strand, within GFF7094 at 14.517 kb on + strand, within GFF7094 at 14.517 kb on + strand, within GFF7094 at 14.517 kb on + strand, within GFF7094 at 14.518 kb on - strand, within GFF7094 at 14.598 kb on + strand, within GFF7094 at 14.606 kb on - strand at 14.616 kb on + strand at 14.616 kb on + strand at 14.617 kb on - strand at 14.617 kb on - strand at 14.631 kb on + strand at 14.631 kb on + strand at 14.632 kb on - strand at 14.632 kb on - strand at 14.632 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 12,608 - GFF7089 0.82 -0.1 12,637 + GFF7089 0.86 -0.9 12,638 - GFF7089 0.86 +0.6 12,718 - +1.3 12,826 + +0.2 12,827 - -0.7 12,845 + GFF7090 0.12 -0.2 12,845 + GFF7090 0.12 +0.4 12,846 - GFF7090 0.12 +0.1 12,848 + GFF7090 0.13 -1.0 12,848 + GFF7090 0.13 -0.8 12,848 + GFF7090 0.13 -0.2 12,848 + GFF7090 0.13 -1.6 12,848 + GFF7090 0.13 -1.1 12,848 + GFF7090 0.13 -0.3 12,849 - GFF7090 0.13 -0.7 12,849 - GFF7090 0.13 -0.1 12,849 - GFF7090 0.13 -1.9 12,953 + GFF7090 0.30 -0.6 12,954 - GFF7090 0.30 +0.9 12,954 - GFF7090 0.30 -0.2 12,954 - GFF7090 0.30 +0.8 13,150 + GFF7090 0.63 -0.0 13,151 - GFF7090 0.64 -0.3 13,168 + GFF7090 0.66 -0.7 13,168 + GFF7090 0.66 -0.7 13,168 + GFF7090 0.66 -0.6 13,168 + GFF7090 0.66 -0.9 13,169 - GFF7090 0.67 +0.5 13,169 - GFF7090 0.67 +1.0 13,169 - GFF7090 0.67 -1.3 13,214 + GFF7090 0.74 -0.0 13,214 + GFF7090 0.74 +1.0 13,214 + GFF7090 0.74 +0.3 13,215 - GFF7090 0.74 -0.6 13,233 - GFF7090 0.77 -0.7 13,241 + GFF7090 0.79 -0.3 13,241 + GFF7090 0.79 -1.0 13,242 - GFF7090 0.79 -0.1 13,332 + -0.7 13,332 + +0.2 13,332 + -0.8 13,333 - -0.6 13,333 - -0.2 13,365 - +2.5 13,365 - -0.6 13,395 + GFF7091 0.11 -0.7 13,409 + GFF7091 0.19 -0.0 13,410 - GFF7091 0.19 +0.1 13,449 - GFF7091 0.41 -0.5 13,602 + GFF7092 0.33 -0.2 13,602 + GFF7092 0.33 -0.3 13,603 - GFF7092 0.34 -1.1 13,603 - GFF7092 0.34 -0.5 13,603 - GFF7092 0.34 -0.6 13,603 - GFF7092 0.34 -0.5 13,603 - GFF7092 0.34 -0.4 13,667 + GFF7092 0.76 -0.3 13,667 + GFF7092 0.76 +0.1 13,668 - GFF7092 0.76 -0.7 13,668 - GFF7092 0.76 -0.4 13,668 - GFF7092 0.76 -0.5 13,668 - GFF7092 0.76 +2.5 13,668 - GFF7092 0.76 -0.2 13,779 + -0.2 13,779 + -0.0 13,779 + -0.3 13,780 - +0.2 13,780 - +0.8 13,783 + +1.3 13,784 - -1.0 13,784 - -2.0 13,784 - -0.5 13,784 - -0.8 13,784 - +0.8 13,841 + +0.5 13,842 - +0.9 13,842 - -0.3 13,842 - +1.0 13,842 - -0.0 13,854 - -1.1 13,871 + +0.9 13,872 - -0.3 13,895 + +1.0 13,895 + -0.1 13,895 + -0.1 13,895 + -0.7 13,895 + +0.5 13,895 + -0.1 13,896 - -0.1 13,896 - -0.3 13,896 - -0.2 13,949 + -2.9 13,950 - -0.5 13,950 - +1.0 13,950 - +0.9 13,950 - -0.8 13,990 + +0.2 13,990 + -0.3 13,990 + +1.9 13,990 + +1.0 13,990 + +0.3 13,991 - -1.0 14,003 + +0.2 14,003 + -0.1 14,004 - +0.3 14,004 - -1.7 14,167 + -1.2 14,167 + -0.7 14,168 - +0.0 14,168 - +0.6 14,168 - +1.6 14,168 - -0.5 14,168 - +0.0 14,168 - -1.8 14,168 - -0.7 14,250 - GFF7093 0.14 +0.2 14,383 + +0.1 14,383 + -0.1 14,383 + +0.1 14,383 + +0.2 14,384 - -0.5 14,384 - -0.7 14,412 - -0.7 14,517 + GFF7094 0.45 +0.3 14,517 + GFF7094 0.45 +2.2 14,517 + GFF7094 0.45 -0.3 14,517 + GFF7094 0.45 -0.2 14,518 - GFF7094 0.46 -0.5 14,598 + GFF7094 0.89 -0.1 14,606 - -1.3 14,616 + -1.1 14,616 + -0.1 14,617 - +0.0 14,617 - -0.2 14,631 + -0.0 14,631 + -0.8 14,632 - -0.8 14,632 - -1.5 14,632 - -0.2
Or see this region's nucleotide sequence