Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF700 and GFF701 are separated by 4 nucleotides GFF701 and GFF702 are separated by 83 nucleotides GFF702 and GFF703 are separated by 219 nucleotides
GFF700 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 755,041 to 756,987
GFF700
GFF701 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 756,992 to 757,867
GFF701
GFF702 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 757,951 to 759,180
GFF702
GFF703 - Efflux ABC transporter, permease/ATP-binding protein Atu2242, at 759,400 to 761,256
GFF703
Position (kb)
757
758
759
760 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 757.084 kb on - strand, within GFF701 at 757.098 kb on + strand, within GFF701 at 757.098 kb on + strand, within GFF701 at 757.099 kb on - strand, within GFF701 at 757.378 kb on - strand, within GFF701 at 757.378 kb on - strand, within GFF701 at 757.689 kb on + strand, within GFF701 at 757.689 kb on + strand, within GFF701 at 757.690 kb on - strand, within GFF701 at 757.690 kb on - strand, within GFF701 at 757.794 kb on + strand at 757.794 kb on + strand at 757.815 kb on + strand at 757.816 kb on - strand at 757.816 kb on - strand at 757.816 kb on - strand at 757.952 kb on + strand at 757.952 kb on + strand at 757.952 kb on + strand at 757.952 kb on + strand at 757.953 kb on - strand at 757.953 kb on - strand at 757.953 kb on - strand at 757.953 kb on - strand at 758.120 kb on + strand, within GFF702 at 758.120 kb on + strand, within GFF702 at 758.120 kb on + strand, within GFF702 at 758.121 kb on - strand, within GFF702 at 758.121 kb on - strand, within GFF702 at 758.121 kb on - strand, within GFF702 at 758.121 kb on - strand, within GFF702 at 758.121 kb on - strand, within GFF702 at 758.121 kb on - strand, within GFF702 at 758.225 kb on + strand, within GFF702 at 758.225 kb on + strand, within GFF702 at 758.225 kb on + strand, within GFF702 at 758.225 kb on + strand, within GFF702 at 758.225 kb on + strand, within GFF702 at 758.226 kb on - strand, within GFF702 at 758.226 kb on - strand, within GFF702 at 758.226 kb on - strand, within GFF702 at 758.237 kb on + strand, within GFF702 at 758.238 kb on - strand, within GFF702 at 758.238 kb on - strand, within GFF702 at 758.238 kb on - strand, within GFF702 at 758.238 kb on - strand, within GFF702 at 758.499 kb on - strand, within GFF702 at 758.499 kb on - strand, within GFF702 at 758.648 kb on + strand, within GFF702 at 758.649 kb on - strand, within GFF702 at 758.649 kb on - strand, within GFF702 at 758.759 kb on + strand, within GFF702 at 758.882 kb on + strand, within GFF702 at 758.882 kb on + strand, within GFF702 at 758.882 kb on + strand, within GFF702 at 758.882 kb on + strand, within GFF702 at 758.883 kb on - strand, within GFF702 at 758.883 kb on - strand, within GFF702 at 758.883 kb on - strand, within GFF702 at 758.883 kb on - strand, within GFF702 at 758.883 kb on - strand, within GFF702 at 758.883 kb on - strand, within GFF702 at 759.260 kb on + strand at 759.261 kb on - strand at 759.261 kb on - strand at 759.368 kb on - strand at 759.434 kb on - strand at 759.793 kb on + strand, within GFF703 at 759.793 kb on + strand, within GFF703 at 759.793 kb on + strand, within GFF703 at 759.794 kb on - strand, within GFF703 at 759.794 kb on - strand, within GFF703 at 759.794 kb on - strand, within GFF703 at 759.794 kb on - strand, within GFF703 at 759.901 kb on + strand, within GFF703 at 759.901 kb on + strand, within GFF703 at 759.901 kb on + strand, within GFF703 at 759.901 kb on + strand, within GFF703 at 759.901 kb on + strand, within GFF703 at 759.901 kb on + strand, within GFF703 at 759.902 kb on - strand, within GFF703 at 759.902 kb on - strand, within GFF703 at 759.902 kb on - strand, within GFF703 at 759.902 kb on - strand, within GFF703 at 759.902 kb on - strand, within GFF703 at 759.902 kb on - strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.979 kb on + strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.980 kb on - strand, within GFF703 at 759.997 kb on + strand, within GFF703 at 759.997 kb on + strand, within GFF703 at 759.997 kb on + strand, within GFF703 at 759.997 kb on + strand, within GFF703 at 759.997 kb on + strand, within GFF703 at 759.997 kb on + strand, within GFF703 at 759.998 kb on - strand, within GFF703 at 759.998 kb on - strand, within GFF703 at 759.998 kb on - strand, within GFF703 at 759.998 kb on - strand, within GFF703 at 759.998 kb on - strand, within GFF703 at 760.078 kb on + strand, within GFF703 at 760.078 kb on + strand, within GFF703 at 760.078 kb on + strand, within GFF703 at 760.078 kb on + strand, within GFF703 at 760.078 kb on + strand, within GFF703 at 760.079 kb on - strand, within GFF703 at 760.079 kb on - strand, within GFF703 at 760.079 kb on - strand, within GFF703
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 757,084 - GFF701 0.11 -0.4 757,098 + GFF701 0.12 -1.9 757,098 + GFF701 0.12 -0.4 757,099 - GFF701 0.12 +0.3 757,378 - GFF701 0.44 +0.2 757,378 - GFF701 0.44 -0.9 757,689 + GFF701 0.80 +0.3 757,689 + GFF701 0.80 -1.3 757,690 - GFF701 0.80 -0.2 757,690 - GFF701 0.80 -0.3 757,794 + -0.6 757,794 + +0.1 757,815 + -1.3 757,816 - +0.8 757,816 - -0.2 757,816 - -0.6 757,952 + -1.1 757,952 + +0.0 757,952 + +1.6 757,952 + -0.5 757,953 - +0.0 757,953 - -1.1 757,953 - -0.1 757,953 - -0.2 758,120 + GFF702 0.14 +0.6 758,120 + GFF702 0.14 +0.1 758,120 + GFF702 0.14 +0.8 758,121 - GFF702 0.14 -0.7 758,121 - GFF702 0.14 +0.1 758,121 - GFF702 0.14 -0.1 758,121 - GFF702 0.14 -0.2 758,121 - GFF702 0.14 -0.6 758,121 - GFF702 0.14 -1.7 758,225 + GFF702 0.22 +1.3 758,225 + GFF702 0.22 -0.1 758,225 + GFF702 0.22 +0.7 758,225 + GFF702 0.22 -1.5 758,225 + GFF702 0.22 -0.7 758,226 - GFF702 0.22 -0.1 758,226 - GFF702 0.22 +1.3 758,226 - GFF702 0.22 -0.3 758,237 + GFF702 0.23 +0.1 758,238 - GFF702 0.23 -0.2 758,238 - GFF702 0.23 -0.3 758,238 - GFF702 0.23 -0.3 758,238 - GFF702 0.23 -0.6 758,499 - GFF702 0.45 +1.6 758,499 - GFF702 0.45 -0.2 758,648 + GFF702 0.57 +0.3 758,649 - GFF702 0.57 -0.3 758,649 - GFF702 0.57 -0.2 758,759 + GFF702 0.66 -1.3 758,882 + GFF702 0.76 -0.3 758,882 + GFF702 0.76 -0.5 758,882 + GFF702 0.76 +0.9 758,882 + GFF702 0.76 -0.1 758,883 - GFF702 0.76 -0.1 758,883 - GFF702 0.76 -0.1 758,883 - GFF702 0.76 +1.1 758,883 - GFF702 0.76 -0.5 758,883 - GFF702 0.76 +0.9 758,883 - GFF702 0.76 +1.5 759,260 + -1.4 759,261 - +0.4 759,261 - +0.7 759,368 - -0.6 759,434 - -0.5 759,793 + GFF703 0.21 +0.3 759,793 + GFF703 0.21 +0.2 759,793 + GFF703 0.21 -1.7 759,794 - GFF703 0.21 +0.0 759,794 - GFF703 0.21 +0.1 759,794 - GFF703 0.21 +0.5 759,794 - GFF703 0.21 -1.3 759,901 + GFF703 0.27 +0.1 759,901 + GFF703 0.27 -1.2 759,901 + GFF703 0.27 -1.2 759,901 + GFF703 0.27 -2.8 759,901 + GFF703 0.27 -1.1 759,901 + GFF703 0.27 -1.0 759,902 - GFF703 0.27 +0.1 759,902 - GFF703 0.27 -0.4 759,902 - GFF703 0.27 +1.7 759,902 - GFF703 0.27 -1.1 759,902 - GFF703 0.27 -0.6 759,902 - GFF703 0.27 -0.5 759,979 + GFF703 0.31 +0.3 759,979 + GFF703 0.31 -0.2 759,979 + GFF703 0.31 +0.3 759,979 + GFF703 0.31 -0.1 759,979 + GFF703 0.31 -0.3 759,979 + GFF703 0.31 +0.0 759,979 + GFF703 0.31 +1.3 759,979 + GFF703 0.31 +0.8 759,979 + GFF703 0.31 +1.3 759,979 + GFF703 0.31 +0.0 759,979 + GFF703 0.31 -0.5 759,980 - GFF703 0.31 -2.3 759,980 - GFF703 0.31 +0.3 759,980 - GFF703 0.31 +0.3 759,980 - GFF703 0.31 -1.5 759,980 - GFF703 0.31 -1.2 759,980 - GFF703 0.31 +0.3 759,980 - GFF703 0.31 -0.3 759,980 - GFF703 0.31 +1.3 759,997 + GFF703 0.32 -2.0 759,997 + GFF703 0.32 +2.0 759,997 + GFF703 0.32 -0.0 759,997 + GFF703 0.32 -0.7 759,997 + GFF703 0.32 -1.3 759,997 + GFF703 0.32 +0.3 759,998 - GFF703 0.32 +0.0 759,998 - GFF703 0.32 -1.2 759,998 - GFF703 0.32 -0.2 759,998 - GFF703 0.32 +0.9 759,998 - GFF703 0.32 +1.7 760,078 + GFF703 0.37 -0.6 760,078 + GFF703 0.37 +0.2 760,078 + GFF703 0.37 -0.2 760,078 + GFF703 0.37 -1.0 760,078 + GFF703 0.37 +0.4 760,079 - GFF703 0.37 -0.9 760,079 - GFF703 0.37 -0.2 760,079 - GFF703 0.37 +0.1
Or see this region's nucleotide sequence