Strain Fitness in Variovorax sp. SCN45 around GFF6986

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6985 and GFF6986 are separated by 605 nucleotidesGFF6986 and GFF6987 are separated by 37 nucleotidesGFF6987 and GFF6988 overlap by 4 nucleotides GFF6985 - Flagellar biosynthesis protein FliP, at 3,646 to 4,419 GFF6985 GFF6986 - hypothetical protein, at 5,025 to 5,522 GFF6986 GFF6987 - hypothetical protein, at 5,560 to 6,498 GFF6987 GFF6988 - Flagellar hook protein FlgE, at 6,495 to 7,703 GFF6988 Position (kb) 5 6Strain fitness (log2 ratio) -1 0 1at 4.204 kb on + strand, within GFF6985at 4.801 kb on + strandat 4.908 kb on + strandat 5.464 kb on - strand, within GFF6986at 5.640 kb on + strandat 5.641 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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4,204 + GFF6985 0.72 -0.6
4,801 + +0.4
4,908 + -1.1
5,464 - GFF6986 0.88 -1.7
5,640 + +0.6
5,641 - -0.0

Or see this region's nucleotide sequence