Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6886 and GFF6887 are separated by 122 nucleotides GFF6887 and GFF6888 overlap by 4 nucleotides GFF6888 and GFF6889 are separated by 53 nucleotides
GFF6886 - DNA-binding transcriptional regulator, MocR family / aminotransferase domain, at 19,471 to 20,916
GFF6886
GFF6887 - Transporter, LysE family, at 21,039 to 21,638
GFF6887
GFF6888 - Permease of the drug/metabolite transporter (DMT) superfamily, at 21,635 to 22,618
GFF6888
GFF6889 - Alpha-aminoadipate aminotransferase (EC 2.6.1.39) @ Leucine transaminase (EC 2.6.1.6) @ Valine transaminase, at 22,672 to 23,874
GFF6889
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 20.702 kb on + strand, within GFF6886 at 20.702 kb on + strand, within GFF6886 at 20.702 kb on + strand, within GFF6886 at 20.703 kb on - strand, within GFF6886 at 20.756 kb on + strand, within GFF6886 at 20.756 kb on + strand, within GFF6886 at 20.757 kb on - strand, within GFF6886 at 20.757 kb on - strand, within GFF6886 at 20.757 kb on - strand, within GFF6886 at 20.774 kb on + strand at 20.775 kb on - strand at 20.775 kb on - strand at 20.927 kb on - strand at 20.971 kb on + strand at 20.971 kb on + strand at 20.971 kb on + strand at 21.000 kb on + strand at 21.000 kb on + strand at 21.001 kb on - strand at 21.001 kb on - strand at 21.011 kb on + strand at 21.011 kb on + strand at 21.011 kb on + strand at 21.012 kb on - strand at 21.012 kb on - strand at 21.026 kb on + strand at 21.026 kb on + strand at 21.027 kb on - strand at 21.027 kb on - strand at 21.282 kb on + strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.283 kb on - strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.300 kb on + strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.301 kb on - strand, within GFF6887 at 21.519 kb on + strand, within GFF6887 at 21.519 kb on + strand, within GFF6887 at 21.519 kb on + strand, within GFF6887 at 21.520 kb on - strand, within GFF6887 at 21.520 kb on - strand, within GFF6887 at 21.520 kb on - strand, within GFF6887 at 21.520 kb on - strand, within GFF6887 at 21.520 kb on - strand, within GFF6887 at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.634 kb on + strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.635 kb on - strand at 21.917 kb on + strand, within GFF6888 at 21.918 kb on - strand, within GFF6888 at 21.918 kb on - strand, within GFF6888 at 22.166 kb on + strand, within GFF6888 at 22.166 kb on + strand, within GFF6888 at 22.166 kb on + strand, within GFF6888 at 22.166 kb on + strand, within GFF6888 at 22.167 kb on - strand, within GFF6888 at 22.167 kb on - strand, within GFF6888 at 22.167 kb on - strand, within GFF6888 at 22.167 kb on - strand, within GFF6888 at 22.167 kb on - strand, within GFF6888 at 22.167 kb on - strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.172 kb on + strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.173 kb on - strand, within GFF6888 at 22.258 kb on + strand, within GFF6888 at 22.316 kb on + strand, within GFF6888 at 22.316 kb on + strand, within GFF6888 at 22.316 kb on + strand, within GFF6888 at 22.316 kb on + strand, within GFF6888 at 22.316 kb on + strand, within GFF6888 at 22.317 kb on - strand, within GFF6888 at 22.317 kb on - strand, within GFF6888 at 22.317 kb on - strand, within GFF6888 at 22.317 kb on - strand, within GFF6888 at 22.317 kb on - strand, within GFF6888 at 22.828 kb on + strand, within GFF6889 at 22.832 kb on + strand, within GFF6889 at 22.832 kb on + strand, within GFF6889 at 22.832 kb on + strand, within GFF6889 at 22.832 kb on + strand, within GFF6889 at 22.832 kb on + strand, within GFF6889 at 22.832 kb on + strand, within GFF6889 at 22.833 kb on - strand, within GFF6889 at 22.833 kb on - strand, within GFF6889 at 22.833 kb on - strand, within GFF6889 at 22.833 kb on - strand, within GFF6889 at 22.833 kb on - strand, within GFF6889 at 22.864 kb on + strand, within GFF6889 at 22.864 kb on + strand, within GFF6889 at 22.864 kb on + strand, within GFF6889 at 22.864 kb on + strand, within GFF6889 at 22.864 kb on + strand, within GFF6889 at 22.864 kb on + strand, within GFF6889 at 22.864 kb on + strand, within GFF6889 at 22.865 kb on - strand, within GFF6889 at 22.865 kb on - strand, within GFF6889 at 22.865 kb on - strand, within GFF6889 at 22.882 kb on + strand, within GFF6889 at 22.883 kb on - strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.035 kb on + strand, within GFF6889 at 23.036 kb on - strand, within GFF6889 at 23.036 kb on - strand, within GFF6889 at 23.036 kb on - strand, within GFF6889 at 23.036 kb on - strand, within GFF6889 at 23.155 kb on + strand, within GFF6889 at 23.156 kb on - strand, within GFF6889 at 23.156 kb on - strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.338 kb on + strand, within GFF6889 at 23.339 kb on - strand, within GFF6889 at 23.339 kb on - strand, within GFF6889 at 23.339 kb on - strand, within GFF6889 at 23.339 kb on - strand, within GFF6889 at 23.339 kb on - strand, within GFF6889 at 23.410 kb on + strand, within GFF6889 at 23.411 kb on - strand, within GFF6889 at 23.411 kb on - strand, within GFF6889 at 23.411 kb on - strand, within GFF6889 at 23.411 kb on - strand, within GFF6889 at 23.536 kb on + strand, within GFF6889 at 23.536 kb on + strand, within GFF6889 at 23.536 kb on + strand, within GFF6889 at 23.536 kb on + strand, within GFF6889 at 23.536 kb on + strand, within GFF6889 at 23.537 kb on - strand, within GFF6889 at 23.537 kb on - strand, within GFF6889 at 23.537 kb on - strand, within GFF6889
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 20,702 + GFF6886 0.85 +1.4 20,702 + GFF6886 0.85 -1.3 20,702 + GFF6886 0.85 -0.3 20,703 - GFF6886 0.85 +1.2 20,756 + GFF6886 0.89 -1.0 20,756 + GFF6886 0.89 -0.1 20,757 - GFF6886 0.89 -0.7 20,757 - GFF6886 0.89 -0.5 20,757 - GFF6886 0.89 -0.2 20,774 + +0.5 20,775 - -0.9 20,775 - -0.6 20,927 - +0.4 20,971 + +0.2 20,971 + +0.2 20,971 + -0.5 21,000 + +0.6 21,000 + +1.8 21,001 - -1.2 21,001 - -0.7 21,011 + +0.4 21,011 + -0.7 21,011 + -2.7 21,012 - -1.8 21,012 - +0.0 21,026 + +0.2 21,026 + -0.3 21,027 - -0.1 21,027 - +0.4 21,282 + GFF6887 0.41 +0.3 21,283 - GFF6887 0.41 +0.8 21,283 - GFF6887 0.41 +0.2 21,283 - GFF6887 0.41 +0.4 21,283 - GFF6887 0.41 -0.4 21,283 - GFF6887 0.41 -0.2 21,283 - GFF6887 0.41 -0.2 21,283 - GFF6887 0.41 -0.2 21,283 - GFF6887 0.41 -0.5 21,300 + GFF6887 0.43 -0.4 21,300 + GFF6887 0.43 +0.7 21,300 + GFF6887 0.43 +0.7 21,300 + GFF6887 0.43 -0.3 21,300 + GFF6887 0.43 -0.0 21,300 + GFF6887 0.43 -0.5 21,300 + GFF6887 0.43 -0.7 21,300 + GFF6887 0.43 +0.9 21,300 + GFF6887 0.43 +0.2 21,300 + GFF6887 0.43 +0.2 21,300 + GFF6887 0.43 -0.4 21,300 + GFF6887 0.43 -0.1 21,300 + GFF6887 0.43 -0.3 21,300 + GFF6887 0.43 -1.3 21,301 - GFF6887 0.44 -0.1 21,301 - GFF6887 0.44 +0.5 21,301 - GFF6887 0.44 -0.5 21,301 - GFF6887 0.44 -0.1 21,301 - GFF6887 0.44 +0.9 21,301 - GFF6887 0.44 -1.5 21,301 - GFF6887 0.44 +0.7 21,301 - GFF6887 0.44 -0.3 21,301 - GFF6887 0.44 +1.5 21,301 - GFF6887 0.44 +0.7 21,301 - GFF6887 0.44 -2.0 21,301 - GFF6887 0.44 -0.1 21,301 - GFF6887 0.44 +0.2 21,519 + GFF6887 0.80 -0.7 21,519 + GFF6887 0.80 -0.7 21,519 + GFF6887 0.80 -0.8 21,520 - GFF6887 0.80 -0.2 21,520 - GFF6887 0.80 -0.0 21,520 - GFF6887 0.80 -1.7 21,520 - GFF6887 0.80 -0.0 21,520 - GFF6887 0.80 -1.0 21,634 + -1.0 21,634 + +0.5 21,634 + +0.5 21,634 + -0.1 21,634 + -0.7 21,634 + -0.6 21,634 + +1.0 21,634 + +0.1 21,634 + -0.9 21,634 + -0.2 21,634 + +0.5 21,634 + -0.2 21,634 + -0.0 21,634 + +0.5 21,635 - -0.6 21,635 - +0.2 21,635 - +0.0 21,635 - -0.2 21,635 - -0.0 21,635 - -0.2 21,635 - +0.5 21,635 - -0.9 21,635 - +1.8 21,635 - +0.8 21,917 + GFF6888 0.29 -1.4 21,918 - GFF6888 0.29 +0.8 21,918 - GFF6888 0.29 +0.8 22,166 + GFF6888 0.54 +0.5 22,166 + GFF6888 0.54 +0.2 22,166 + GFF6888 0.54 +0.3 22,166 + GFF6888 0.54 +0.4 22,167 - GFF6888 0.54 -0.0 22,167 - GFF6888 0.54 -0.3 22,167 - GFF6888 0.54 -0.4 22,167 - GFF6888 0.54 -0.2 22,167 - GFF6888 0.54 -0.6 22,167 - GFF6888 0.54 -1.1 22,172 + GFF6888 0.55 +0.8 22,172 + GFF6888 0.55 -0.3 22,172 + GFF6888 0.55 -0.1 22,172 + GFF6888 0.55 +0.2 22,172 + GFF6888 0.55 -0.5 22,172 + GFF6888 0.55 -0.7 22,172 + GFF6888 0.55 -0.8 22,172 + GFF6888 0.55 +1.0 22,172 + GFF6888 0.55 -0.1 22,173 - GFF6888 0.55 +0.6 22,173 - GFF6888 0.55 +1.5 22,173 - GFF6888 0.55 -1.0 22,173 - GFF6888 0.55 +0.2 22,173 - GFF6888 0.55 +0.3 22,173 - GFF6888 0.55 -1.0 22,173 - GFF6888 0.55 -0.8 22,173 - GFF6888 0.55 +1.0 22,258 + GFF6888 0.63 -0.4 22,316 + GFF6888 0.69 +3.1 22,316 + GFF6888 0.69 +1.9 22,316 + GFF6888 0.69 -0.7 22,316 + GFF6888 0.69 -0.1 22,316 + GFF6888 0.69 +0.2 22,317 - GFF6888 0.69 +0.5 22,317 - GFF6888 0.69 -0.4 22,317 - GFF6888 0.69 +1.8 22,317 - GFF6888 0.69 -0.4 22,317 - GFF6888 0.69 -0.1 22,828 + GFF6889 0.13 -0.2 22,832 + GFF6889 0.13 -1.2 22,832 + GFF6889 0.13 -1.1 22,832 + GFF6889 0.13 +0.2 22,832 + GFF6889 0.13 +0.2 22,832 + GFF6889 0.13 -0.5 22,832 + GFF6889 0.13 +0.0 22,833 - GFF6889 0.13 +0.2 22,833 - GFF6889 0.13 -0.1 22,833 - GFF6889 0.13 +0.4 22,833 - GFF6889 0.13 -1.1 22,833 - GFF6889 0.13 +0.3 22,864 + GFF6889 0.16 -0.2 22,864 + GFF6889 0.16 -0.9 22,864 + GFF6889 0.16 +0.4 22,864 + GFF6889 0.16 -0.2 22,864 + GFF6889 0.16 +0.5 22,864 + GFF6889 0.16 -0.5 22,864 + GFF6889 0.16 -1.4 22,865 - GFF6889 0.16 +0.4 22,865 - GFF6889 0.16 -0.6 22,865 - GFF6889 0.16 -0.2 22,882 + GFF6889 0.17 +0.5 22,883 - GFF6889 0.18 -0.1 23,035 + GFF6889 0.30 +0.2 23,035 + GFF6889 0.30 -1.3 23,035 + GFF6889 0.30 -0.2 23,035 + GFF6889 0.30 +0.4 23,035 + GFF6889 0.30 -0.2 23,035 + GFF6889 0.30 -0.3 23,035 + GFF6889 0.30 -0.4 23,035 + GFF6889 0.30 -0.5 23,035 + GFF6889 0.30 -0.8 23,036 - GFF6889 0.30 -0.3 23,036 - GFF6889 0.30 +0.4 23,036 - GFF6889 0.30 -1.2 23,036 - GFF6889 0.30 -0.8 23,155 + GFF6889 0.40 -3.4 23,156 - GFF6889 0.40 +1.0 23,156 - GFF6889 0.40 +0.4 23,338 + GFF6889 0.55 +0.2 23,338 + GFF6889 0.55 -1.0 23,338 + GFF6889 0.55 -1.0 23,338 + GFF6889 0.55 -0.6 23,338 + GFF6889 0.55 -1.0 23,338 + GFF6889 0.55 -0.5 23,338 + GFF6889 0.55 -0.7 23,338 + GFF6889 0.55 +0.0 23,338 + GFF6889 0.55 -1.0 23,339 - GFF6889 0.55 +0.0 23,339 - GFF6889 0.55 -1.1 23,339 - GFF6889 0.55 -0.1 23,339 - GFF6889 0.55 -0.2 23,339 - GFF6889 0.55 -2.6 23,410 + GFF6889 0.61 -1.4 23,411 - GFF6889 0.61 -0.6 23,411 - GFF6889 0.61 -0.1 23,411 - GFF6889 0.61 +0.7 23,411 - GFF6889 0.61 -1.3 23,536 + GFF6889 0.72 +0.1 23,536 + GFF6889 0.72 -0.3 23,536 + GFF6889 0.72 -1.2 23,536 + GFF6889 0.72 +0.5 23,536 + GFF6889 0.72 -0.9 23,537 - GFF6889 0.72 +0.8 23,537 - GFF6889 0.72 +1.2 23,537 - GFF6889 0.72 +0.2
Or see this region's nucleotide sequence