Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF66 and GFF67 overlap by 4 nucleotides GFF67 and GFF68 are separated by 31 nucleotides
GFF66 - hypothetical protein, at 82,456 to 83,709
GFF66
GFF67 - Glycosyl transferase, group 1, at 83,706 to 84,938
GFF67
GFF68 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 84,970 to 86,088
GFF68
Position (kb)
83
84
85 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 82.876 kb on + strand, within GFF66 at 83.122 kb on + strand, within GFF66 at 83.122 kb on + strand, within GFF66 at 83.191 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.467 kb on + strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.468 kb on - strand, within GFF66 at 83.564 kb on - strand, within GFF66 at 83.602 kb on + strand at 83.602 kb on + strand at 83.602 kb on + strand at 83.603 kb on - strand at 83.721 kb on + strand at 83.721 kb on + strand at 83.721 kb on + strand at 83.722 kb on - strand at 83.763 kb on + strand at 83.764 kb on - strand at 83.854 kb on - strand, within GFF67 at 83.877 kb on + strand, within GFF67 at 83.878 kb on - strand, within GFF67 at 84.126 kb on + strand, within GFF67 at 84.126 kb on + strand, within GFF67 at 84.127 kb on - strand, within GFF67 at 84.408 kb on + strand, within GFF67 at 84.408 kb on + strand, within GFF67 at 84.409 kb on - strand, within GFF67 at 84.409 kb on - strand, within GFF67 at 84.861 kb on + strand at 85.052 kb on - strand at 85.052 kb on - strand at 85.052 kb on - strand at 85.265 kb on - strand, within GFF68 at 85.267 kb on + strand, within GFF68 at 85.360 kb on + strand, within GFF68 at 85.360 kb on + strand, within GFF68 at 85.361 kb on - strand, within GFF68 at 85.420 kb on + strand, within GFF68 at 85.420 kb on + strand, within GFF68 at 85.505 kb on - strand, within GFF68 at 85.582 kb on + strand, within GFF68 at 85.582 kb on + strand, within GFF68 at 85.583 kb on - strand, within GFF68 at 85.583 kb on - strand, within GFF68 at 85.616 kb on - strand, within GFF68 at 85.639 kb on + strand, within GFF68 at 85.672 kb on + strand, within GFF68 at 85.672 kb on + strand, within GFF68 at 85.673 kb on - strand, within GFF68 at 85.673 kb on - strand, within GFF68
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 82,876 + GFF66 0.33 +0.0 83,122 + GFF66 0.53 -0.1 83,122 + GFF66 0.53 -1.0 83,191 + GFF66 0.59 +0.5 83,467 + GFF66 0.81 -0.3 83,467 + GFF66 0.81 -1.7 83,467 + GFF66 0.81 -1.2 83,467 + GFF66 0.81 +0.4 83,468 - GFF66 0.81 +0.9 83,468 - GFF66 0.81 -1.1 83,468 - GFF66 0.81 +0.7 83,468 - GFF66 0.81 -1.5 83,564 - GFF66 0.88 -0.9 83,602 + -0.4 83,602 + -0.7 83,602 + -2.2 83,603 - -0.9 83,721 + -0.2 83,721 + +0.0 83,721 + -0.4 83,722 - +0.4 83,763 + -0.3 83,764 - -1.1 83,854 - GFF67 0.12 +0.7 83,877 + GFF67 0.14 +0.6 83,878 - GFF67 0.14 -0.8 84,126 + GFF67 0.34 -0.3 84,126 + GFF67 0.34 -0.7 84,127 - GFF67 0.34 +1.2 84,408 + GFF67 0.57 -2.5 84,408 + GFF67 0.57 -0.2 84,409 - GFF67 0.57 +1.0 84,409 - GFF67 0.57 -0.7 84,861 + +1.0 85,052 - -0.2 85,052 - +0.7 85,052 - +0.3 85,265 - GFF68 0.26 +1.9 85,267 + GFF68 0.27 -0.3 85,360 + GFF68 0.35 +1.9 85,360 + GFF68 0.35 -0.5 85,361 - GFF68 0.35 -0.2 85,420 + GFF68 0.40 -0.7 85,420 + GFF68 0.40 +0.0 85,505 - GFF68 0.48 -0.5 85,582 + GFF68 0.55 -2.5 85,582 + GFF68 0.55 -1.0 85,583 - GFF68 0.55 +1.3 85,583 - GFF68 0.55 +0.4 85,616 - GFF68 0.58 -0.3 85,639 + GFF68 0.60 -0.7 85,672 + GFF68 0.63 -0.5 85,672 + GFF68 0.63 -0.0 85,673 - GFF68 0.63 -0.1 85,673 - GFF68 0.63 -0.6
Or see this region's nucleotide sequence