Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6646 and GFF6647 are separated by 42 nucleotides GFF6647 and GFF6648 are separated by 86 nucleotides GFF6648 and GFF6649 are separated by 11 nucleotides GFF6649 and GFF6650 are separated by 156 nucleotides
GFF6646 - no description, at 19,915 to 20,541
GFF6646
GFF6647 - Two-component transcriptional response regulator, LuxR family, at 20,584 to 21,288
GFF6647
GFF6648 - hypothetical protein, at 21,375 to 23,114
GFF6648
GFF6649 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 23,126 to 23,950
GFF6649
GFF6650 - no description, at 24,107 to 24,991
GFF6650
Position (kb) 21
22
23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 20.422 kb on + strand, within GFF6646 at 20.422 kb on + strand, within GFF6646 at 20.422 kb on + strand, within GFF6646 at 20.422 kb on + strand, within GFF6646 at 20.422 kb on + strand, within GFF6646 at 20.423 kb on - strand, within GFF6646 at 20.423 kb on - strand, within GFF6646 at 20.423 kb on - strand, within GFF6646 at 20.456 kb on - strand, within GFF6646 at 20.456 kb on - strand, within GFF6646 at 20.456 kb on - strand, within GFF6646 at 20.482 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.512 kb on + strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.513 kb on - strand at 20.539 kb on + strand at 20.539 kb on + strand at 20.677 kb on + strand, within GFF6647 at 20.677 kb on + strand, within GFF6647 at 20.677 kb on + strand, within GFF6647 at 20.678 kb on - strand, within GFF6647 at 20.767 kb on + strand, within GFF6647 at 20.767 kb on + strand, within GFF6647 at 20.768 kb on - strand, within GFF6647 at 20.926 kb on + strand, within GFF6647 at 20.926 kb on + strand, within GFF6647 at 20.927 kb on - strand, within GFF6647 at 21.204 kb on + strand, within GFF6647 at 21.204 kb on + strand, within GFF6647 at 21.205 kb on - strand, within GFF6647 at 21.217 kb on + strand, within GFF6647 at 21.217 kb on + strand, within GFF6647 at 21.217 kb on + strand, within GFF6647 at 21.217 kb on + strand, within GFF6647 at 21.217 kb on + strand, within GFF6647 at 21.217 kb on + strand, within GFF6647 at 21.217 kb on + strand, within GFF6647 at 21.218 kb on - strand at 21.218 kb on - strand at 21.486 kb on + strand at 21.487 kb on - strand at 21.487 kb on - strand at 21.487 kb on - strand at 21.543 kb on + strand at 21.543 kb on + strand at 21.543 kb on + strand at 21.543 kb on + strand at 21.543 kb on + strand at 21.544 kb on - strand at 21.717 kb on + strand, within GFF6648 at 21.865 kb on + strand, within GFF6648 at 21.866 kb on - strand, within GFF6648 at 21.933 kb on + strand, within GFF6648 at 21.934 kb on - strand, within GFF6648 at 21.934 kb on - strand, within GFF6648 at 21.934 kb on - strand, within GFF6648 at 21.934 kb on - strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.026 kb on + strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.027 kb on - strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.328 kb on + strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.329 kb on - strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.332 kb on + strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.333 kb on - strand, within GFF6648 at 22.335 kb on + strand, within GFF6648 at 22.335 kb on + strand, within GFF6648 at 22.335 kb on + strand, within GFF6648 at 22.335 kb on + strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.336 kb on - strand, within GFF6648 at 22.353 kb on + strand, within GFF6648 at 22.354 kb on - strand, within GFF6648 at 22.354 kb on - strand, within GFF6648 at 22.354 kb on - strand, within GFF6648 at 23.194 kb on - strand at 23.194 kb on - strand at 23.229 kb on + strand, within GFF6649 at 23.229 kb on + strand, within GFF6649 at 23.229 kb on + strand, within GFF6649 at 23.230 kb on - strand, within GFF6649 at 23.230 kb on - strand, within GFF6649 at 23.230 kb on - strand, within GFF6649 at 23.334 kb on + strand, within GFF6649 at 23.334 kb on + strand, within GFF6649 at 23.335 kb on - strand, within GFF6649 at 23.335 kb on - strand, within GFF6649 at 23.335 kb on - strand, within GFF6649 at 23.335 kb on - strand, within GFF6649 at 23.335 kb on - strand, within GFF6649 at 23.335 kb on - strand, within GFF6649 at 23.335 kb on - strand, within GFF6649 at 23.343 kb on + strand, within GFF6649 at 23.343 kb on + strand, within GFF6649 at 23.343 kb on + strand, within GFF6649 at 23.343 kb on + strand, within GFF6649 at 23.344 kb on - strand, within GFF6649 at 23.344 kb on - strand, within GFF6649 at 23.344 kb on - strand, within GFF6649 at 23.763 kb on + strand, within GFF6649 at 23.763 kb on + strand, within GFF6649 at 23.954 kb on + strand at 23.954 kb on + strand at 23.954 kb on + strand at 23.954 kb on + strand at 23.954 kb on + strand at 23.954 kb on + strand at 23.955 kb on - strand at 23.955 kb on - strand at 23.955 kb on - strand at 23.955 kb on - strand at 23.955 kb on - strand at 23.955 kb on - strand at 23.976 kb on + strand at 24.022 kb on + strand at 24.022 kb on + strand at 24.022 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.026 kb on + strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.027 kb on - strand at 24.038 kb on + strand at 24.038 kb on + strand at 24.063 kb on + strand at 24.064 kb on - strand at 24.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 20,422 + GFF6646 0.81 +0.8 20,422 + GFF6646 0.81 -0.1 20,422 + GFF6646 0.81 +0.1 20,422 + GFF6646 0.81 -0.3 20,422 + GFF6646 0.81 -0.9 20,423 - GFF6646 0.81 -2.2 20,423 - GFF6646 0.81 -0.5 20,423 - GFF6646 0.81 +0.1 20,456 - GFF6646 0.86 +0.5 20,456 - GFF6646 0.86 +0.4 20,456 - GFF6646 0.86 +0.2 20,482 + +0.8 20,512 + +0.1 20,512 + +0.6 20,512 + +0.2 20,512 + -0.5 20,512 + +0.8 20,512 + -0.3 20,512 + -0.5 20,512 + -0.8 20,512 + -0.1 20,512 + -1.1 20,512 + -0.1 20,512 + +0.4 20,512 + -0.9 20,512 + +0.5 20,512 + -0.5 20,512 + +0.6 20,512 + +1.1 20,512 + -0.8 20,512 + -0.9 20,513 - +0.1 20,513 - +0.4 20,513 - -0.4 20,513 - -0.2 20,513 - -0.3 20,513 - +0.7 20,513 - -2.3 20,513 - -0.9 20,513 - -1.1 20,513 - -0.7 20,513 - +1.0 20,513 - -0.2 20,513 - +0.1 20,513 - +0.9 20,513 - +0.0 20,513 - -0.0 20,513 - -0.1 20,513 - -0.5 20,513 - -0.4 20,513 - -1.4 20,513 - -0.1 20,513 - +0.3 20,539 + -0.3 20,539 + +0.1 20,677 + GFF6647 0.13 -0.4 20,677 + GFF6647 0.13 +0.3 20,677 + GFF6647 0.13 +0.1 20,678 - GFF6647 0.13 -0.7 20,767 + GFF6647 0.26 +0.2 20,767 + GFF6647 0.26 -0.2 20,768 - GFF6647 0.26 -0.1 20,926 + GFF6647 0.49 +0.1 20,926 + GFF6647 0.49 -0.8 20,927 - GFF6647 0.49 -0.1 21,204 + GFF6647 0.88 -1.5 21,204 + GFF6647 0.88 -0.9 21,205 - GFF6647 0.88 -0.6 21,217 + GFF6647 0.90 -2.1 21,217 + GFF6647 0.90 -0.1 21,217 + GFF6647 0.90 +0.8 21,217 + GFF6647 0.90 -0.4 21,217 + GFF6647 0.90 +0.0 21,217 + GFF6647 0.90 -1.8 21,217 + GFF6647 0.90 -0.5 21,218 - +0.8 21,218 - -0.1 21,486 + +0.7 21,487 - +0.1 21,487 - -0.2 21,487 - +0.6 21,543 + -0.1 21,543 + +0.0 21,543 + +1.4 21,543 + -1.1 21,543 + +0.6 21,544 - -0.9 21,717 + GFF6648 0.20 +1.4 21,865 + GFF6648 0.28 -0.5 21,866 - GFF6648 0.28 +1.7 21,933 + GFF6648 0.32 +0.6 21,934 - GFF6648 0.32 -0.8 21,934 - GFF6648 0.32 -1.6 21,934 - GFF6648 0.32 -1.0 21,934 - GFF6648 0.32 -0.2 22,026 + GFF6648 0.37 -1.0 22,026 + GFF6648 0.37 +0.1 22,026 + GFF6648 0.37 -1.1 22,026 + GFF6648 0.37 +1.4 22,026 + GFF6648 0.37 -0.4 22,026 + GFF6648 0.37 +1.0 22,026 + GFF6648 0.37 -0.5 22,026 + GFF6648 0.37 +0.2 22,026 + GFF6648 0.37 -0.7 22,026 + GFF6648 0.37 -0.5 22,026 + GFF6648 0.37 -1.0 22,026 + GFF6648 0.37 +1.8 22,026 + GFF6648 0.37 -0.4 22,026 + GFF6648 0.37 -0.9 22,027 - GFF6648 0.37 -0.1 22,027 - GFF6648 0.37 -2.0 22,027 - GFF6648 0.37 -1.2 22,027 - GFF6648 0.37 -0.3 22,027 - GFF6648 0.37 -1.1 22,027 - GFF6648 0.37 -0.1 22,027 - GFF6648 0.37 -0.7 22,027 - GFF6648 0.37 -0.5 22,328 + GFF6648 0.55 -0.9 22,328 + GFF6648 0.55 -0.7 22,328 + GFF6648 0.55 -0.4 22,328 + GFF6648 0.55 +0.0 22,328 + GFF6648 0.55 -0.7 22,328 + GFF6648 0.55 -0.2 22,328 + GFF6648 0.55 -0.1 22,328 + GFF6648 0.55 +1.0 22,329 - GFF6648 0.55 -0.0 22,329 - GFF6648 0.55 -0.2 22,329 - GFF6648 0.55 +1.7 22,329 - GFF6648 0.55 -0.4 22,329 - GFF6648 0.55 -3.4 22,329 - GFF6648 0.55 -0.4 22,329 - GFF6648 0.55 +0.0 22,329 - GFF6648 0.55 -0.2 22,329 - GFF6648 0.55 +1.1 22,332 + GFF6648 0.55 -0.4 22,332 + GFF6648 0.55 -0.9 22,332 + GFF6648 0.55 -1.5 22,332 + GFF6648 0.55 -1.6 22,332 + GFF6648 0.55 +0.1 22,332 + GFF6648 0.55 -0.3 22,332 + GFF6648 0.55 -0.4 22,332 + GFF6648 0.55 -0.5 22,332 + GFF6648 0.55 +0.4 22,332 + GFF6648 0.55 +0.1 22,332 + GFF6648 0.55 -0.0 22,332 + GFF6648 0.55 +0.0 22,333 - GFF6648 0.55 -0.6 22,333 - GFF6648 0.55 -0.7 22,333 - GFF6648 0.55 -0.2 22,333 - GFF6648 0.55 +1.4 22,333 - GFF6648 0.55 -0.3 22,333 - GFF6648 0.55 +0.5 22,333 - GFF6648 0.55 +0.2 22,333 - GFF6648 0.55 +0.2 22,333 - GFF6648 0.55 -0.4 22,333 - GFF6648 0.55 +2.0 22,333 - GFF6648 0.55 +0.1 22,333 - GFF6648 0.55 -0.2 22,333 - GFF6648 0.55 -1.0 22,335 + GFF6648 0.55 -0.3 22,335 + GFF6648 0.55 -0.8 22,335 + GFF6648 0.55 -0.6 22,335 + GFF6648 0.55 +0.3 22,336 - GFF6648 0.55 -1.2 22,336 - GFF6648 0.55 -0.0 22,336 - GFF6648 0.55 -1.4 22,336 - GFF6648 0.55 -0.3 22,336 - GFF6648 0.55 -0.5 22,336 - GFF6648 0.55 -1.0 22,336 - GFF6648 0.55 -1.0 22,336 - GFF6648 0.55 -0.4 22,336 - GFF6648 0.55 +0.2 22,336 - GFF6648 0.55 +0.0 22,336 - GFF6648 0.55 -0.3 22,353 + GFF6648 0.56 +0.7 22,354 - GFF6648 0.56 -1.6 22,354 - GFF6648 0.56 +0.1 22,354 - GFF6648 0.56 +0.7 23,194 - +0.2 23,194 - -0.4 23,229 + GFF6649 0.12 +0.5 23,229 + GFF6649 0.12 -0.3 23,229 + GFF6649 0.12 +0.9 23,230 - GFF6649 0.13 +0.4 23,230 - GFF6649 0.13 +0.3 23,230 - GFF6649 0.13 +0.4 23,334 + GFF6649 0.25 -0.2 23,334 + GFF6649 0.25 +0.4 23,335 - GFF6649 0.25 -0.6 23,335 - GFF6649 0.25 -0.4 23,335 - GFF6649 0.25 -0.5 23,335 - GFF6649 0.25 +0.2 23,335 - GFF6649 0.25 -1.1 23,335 - GFF6649 0.25 -0.5 23,335 - GFF6649 0.25 -0.2 23,343 + GFF6649 0.26 +0.1 23,343 + GFF6649 0.26 -1.4 23,343 + GFF6649 0.26 -1.0 23,343 + GFF6649 0.26 -1.4 23,344 - GFF6649 0.26 +0.0 23,344 - GFF6649 0.26 -0.6 23,344 - GFF6649 0.26 -1.4 23,763 + GFF6649 0.77 -0.1 23,763 + GFF6649 0.77 +1.8 23,954 + -0.5 23,954 + -1.4 23,954 + -1.9 23,954 + +0.3 23,954 + +0.1 23,954 + -0.7 23,955 - -0.3 23,955 - +0.1 23,955 - -1.5 23,955 - -0.4 23,955 - +0.8 23,955 - +0.0 23,976 + +0.4 24,022 + +0.2 24,022 + -0.7 24,022 + -0.5 24,026 + -1.1 24,026 + -0.6 24,026 + +0.0 24,026 + -0.8 24,026 + +0.3 24,026 + +1.0 24,026 + -0.8 24,026 + -0.1 24,026 + -0.2 24,026 + +0.5 24,026 + -1.8 24,027 - -0.9 24,027 - -1.2 24,027 - -0.7 24,027 - +0.4 24,027 - +0.9 24,027 - +0.1 24,027 - +0.1 24,027 - +0.2 24,027 - +0.4 24,027 - -1.7 24,027 - -0.9 24,027 - -0.6 24,027 - -0.4 24,038 + +0.8 24,038 + -1.1 24,063 + -0.3 24,064 - +0.2 24,064 - +1.7
Or see this region's nucleotide sequence