Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6576 and GFF6577 are separated by 12 nucleotides GFF6577 and GFF6578 overlap by 4 nucleotides GFF6578 and GFF6579 overlap by 4 nucleotides GFF6579 and GFF6580 overlap by 4 nucleotides GFF6580 and GFF6581 overlap by 4 nucleotides
GFF6576 - Conjugative transfer protein TrbE, at 17,046 to 19,520
GFF6576
GFF6577 - Conjugative transfer protein TrbD, at 19,533 to 19,805
GFF6577
GFF6578 - Conjugative transfer protein TrbC, at 19,802 to 20,179
GFF6578
GFF6579 - Conjugative transfer protein TrbB, at 20,176 to 21,213
GFF6579
GFF6580 - CopG domain-containing protein, at 21,210 to 21,674
GFF6580
GFF6581 - Coupling protein VirD4, ATPase required for T-DNA transfer, at 21,671 to 23,680
GFF6581
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1 at 19.271 kb on + strand, within GFF6576 at 19.535 kb on - strand at 19.535 kb on - strand at 19.806 kb on - strand at 20.156 kb on - strand at 20.825 kb on + strand, within GFF6579 at 20.825 kb on + strand, within GFF6579 at 20.825 kb on + strand, within GFF6579 at 20.825 kb on + strand, within GFF6579 at 20.826 kb on - strand, within GFF6579 at 21.213 kb on + strand at 21.214 kb on - strand at 21.214 kb on - strand at 21.214 kb on - strand at 21.214 kb on - strand at 21.258 kb on - strand, within GFF6580 at 21.424 kb on + strand, within GFF6580 at 21.425 kb on - strand, within GFF6580 at 21.674 kb on + strand at 21.675 kb on - strand at 21.675 kb on - strand at 21.675 kb on - strand at 21.820 kb on - strand at 21.887 kb on + strand, within GFF6581 at 21.887 kb on + strand, within GFF6581 at 21.887 kb on + strand, within GFF6581 at 21.887 kb on + strand, within GFF6581 at 21.887 kb on + strand, within GFF6581 at 21.887 kb on + strand, within GFF6581 at 21.887 kb on + strand, within GFF6581 at 21.888 kb on - strand, within GFF6581 at 21.888 kb on - strand, within GFF6581 at 21.888 kb on - strand, within GFF6581 at 21.888 kb on - strand, within GFF6581 at 21.888 kb on - strand, within GFF6581 at 21.888 kb on - strand, within GFF6581 at 22.035 kb on + strand, within GFF6581 at 22.198 kb on - strand, within GFF6581 at 22.198 kb on - strand, within GFF6581
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 19,271 + GFF6576 0.90 -0.4 19,535 - -0.2 19,535 - -0.4 19,806 - -1.7 20,156 - -1.3 20,825 + GFF6579 0.63 +0.1 20,825 + GFF6579 0.63 +0.0 20,825 + GFF6579 0.63 -2.3 20,825 + GFF6579 0.63 +1.0 20,826 - GFF6579 0.63 +0.0 21,213 + +1.0 21,214 - +0.6 21,214 - -0.9 21,214 - -1.0 21,214 - -0.4 21,258 - GFF6580 0.10 -0.3 21,424 + GFF6580 0.46 +1.1 21,425 - GFF6580 0.46 +0.1 21,674 + -1.2 21,675 - -1.9 21,675 - -0.7 21,675 - -0.9 21,820 - -0.6 21,887 + GFF6581 0.11 -0.6 21,887 + GFF6581 0.11 +0.4 21,887 + GFF6581 0.11 -1.6 21,887 + GFF6581 0.11 +0.9 21,887 + GFF6581 0.11 -0.9 21,887 + GFF6581 0.11 +0.0 21,887 + GFF6581 0.11 -0.8 21,888 - GFF6581 0.11 -0.1 21,888 - GFF6581 0.11 -1.6 21,888 - GFF6581 0.11 -0.7 21,888 - GFF6581 0.11 +0.0 21,888 - GFF6581 0.11 +0.0 21,888 - GFF6581 0.11 -0.5 22,035 + GFF6581 0.18 -0.4 22,198 - GFF6581 0.26 -0.0 22,198 - GFF6581 0.26 -1.2
Or see this region's nucleotide sequence