Strain Fitness in Variovorax sp. SCN45 around GFF6579

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6576 and GFF6577 are separated by 12 nucleotidesGFF6577 and GFF6578 overlap by 4 nucleotidesGFF6578 and GFF6579 overlap by 4 nucleotidesGFF6579 and GFF6580 overlap by 4 nucleotidesGFF6580 and GFF6581 overlap by 4 nucleotides GFF6576 - Conjugative transfer protein TrbE, at 17,046 to 19,520 GFF6576 GFF6577 - Conjugative transfer protein TrbD, at 19,533 to 19,805 GFF6577 GFF6578 - Conjugative transfer protein TrbC, at 19,802 to 20,179 GFF6578 GFF6579 - Conjugative transfer protein TrbB, at 20,176 to 21,213 GFF6579 GFF6580 - CopG domain-containing protein, at 21,210 to 21,674 GFF6580 GFF6581 - Coupling protein VirD4, ATPase required for T-DNA transfer, at 21,671 to 23,680 GFF6581 Position (kb) 20 21 22Strain fitness (log2 ratio) -2 -1 0 1at 19.271 kb on + strand, within GFF6576at 19.535 kb on - strandat 19.535 kb on - strandat 19.806 kb on - strandat 20.156 kb on - strandat 20.825 kb on + strand, within GFF6579at 20.825 kb on + strand, within GFF6579at 20.825 kb on + strand, within GFF6579at 20.825 kb on + strand, within GFF6579at 20.826 kb on - strand, within GFF6579at 21.213 kb on + strandat 21.214 kb on - strandat 21.214 kb on - strandat 21.214 kb on - strandat 21.214 kb on - strandat 21.258 kb on - strand, within GFF6580at 21.424 kb on + strand, within GFF6580at 21.425 kb on - strand, within GFF6580at 21.674 kb on + strandat 21.675 kb on - strandat 21.675 kb on - strandat 21.675 kb on - strandat 21.820 kb on - strandat 21.887 kb on + strand, within GFF6581at 21.887 kb on + strand, within GFF6581at 21.887 kb on + strand, within GFF6581at 21.887 kb on + strand, within GFF6581at 21.887 kb on + strand, within GFF6581at 21.887 kb on + strand, within GFF6581at 21.887 kb on + strand, within GFF6581at 21.888 kb on - strand, within GFF6581at 21.888 kb on - strand, within GFF6581at 21.888 kb on - strand, within GFF6581at 21.888 kb on - strand, within GFF6581at 21.888 kb on - strand, within GFF6581at 21.888 kb on - strand, within GFF6581at 22.035 kb on + strand, within GFF6581at 22.198 kb on - strand, within GFF6581at 22.198 kb on - strand, within GFF6581

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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19,271 + GFF6576 0.90 -0.4
19,535 - -0.2
19,535 - -0.4
19,806 - -1.7
20,156 - -1.3
20,825 + GFF6579 0.63 +0.1
20,825 + GFF6579 0.63 +0.0
20,825 + GFF6579 0.63 -2.3
20,825 + GFF6579 0.63 +1.0
20,826 - GFF6579 0.63 +0.0
21,213 + +1.0
21,214 - +0.6
21,214 - -0.9
21,214 - -1.0
21,214 - -0.4
21,258 - GFF6580 0.10 -0.3
21,424 + GFF6580 0.46 +1.1
21,425 - GFF6580 0.46 +0.1
21,674 + -1.2
21,675 - -1.9
21,675 - -0.7
21,675 - -0.9
21,820 - -0.6
21,887 + GFF6581 0.11 -0.6
21,887 + GFF6581 0.11 +0.4
21,887 + GFF6581 0.11 -1.6
21,887 + GFF6581 0.11 +0.9
21,887 + GFF6581 0.11 -0.9
21,887 + GFF6581 0.11 +0.0
21,887 + GFF6581 0.11 -0.8
21,888 - GFF6581 0.11 -0.1
21,888 - GFF6581 0.11 -1.6
21,888 - GFF6581 0.11 -0.7
21,888 - GFF6581 0.11 +0.0
21,888 - GFF6581 0.11 +0.0
21,888 - GFF6581 0.11 -0.5
22,035 + GFF6581 0.18 -0.4
22,198 - GFF6581 0.26 -0.0
22,198 - GFF6581 0.26 -1.2

Or see this region's nucleotide sequence