Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6574 and GFF6575 are separated by 12 nucleotides GFF6575 and GFF6576 overlap by 4 nucleotides
GFF6574 - Conjugative transfer protein TrbL, at 14,941 to 16,266
GFF6574
GFF6575 - Conjugative transfer protein TrbJ, at 16,279 to 17,049
GFF6575
GFF6576 - Conjugative transfer protein TrbE, at 17,046 to 19,520
GFF6576
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1
2
3 at 16.040 kb on + strand, within GFF6574 at 16.040 kb on + strand, within GFF6574 at 16.040 kb on + strand, within GFF6574 at 16.041 kb on - strand, within GFF6574 at 16.058 kb on + strand, within GFF6574 at 16.058 kb on + strand, within GFF6574 at 16.058 kb on + strand, within GFF6574 at 16.059 kb on - strand, within GFF6574 at 16.059 kb on - strand, within GFF6574 at 16.059 kb on - strand, within GFF6574 at 16.146 kb on + strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.147 kb on - strand at 16.217 kb on + strand at 16.218 kb on - strand at 16.218 kb on - strand at 16.290 kb on - strand at 16.313 kb on + strand at 16.313 kb on + strand at 16.313 kb on + strand at 16.314 kb on - strand at 16.314 kb on - strand at 16.314 kb on - strand at 16.668 kb on - strand, within GFF6575 at 16.668 kb on - strand, within GFF6575 at 16.668 kb on - strand, within GFF6575 at 16.679 kb on + strand, within GFF6575 at 16.680 kb on - strand, within GFF6575 at 16.718 kb on + strand, within GFF6575 at 16.849 kb on + strand, within GFF6575 at 16.849 kb on + strand, within GFF6575 at 16.850 kb on - strand, within GFF6575 at 16.850 kb on - strand, within GFF6575 at 17.300 kb on - strand, within GFF6576 at 17.300 kb on - strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.302 kb on + strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.303 kb on - strand, within GFF6576 at 17.305 kb on + strand, within GFF6576 at 17.305 kb on + strand, within GFF6576 at 17.305 kb on + strand, within GFF6576 at 17.306 kb on - strand, within GFF6576 at 17.306 kb on - strand, within GFF6576 at 17.306 kb on - strand, within GFF6576 at 17.306 kb on - strand, within GFF6576 at 17.371 kb on + strand, within GFF6576 at 17.371 kb on + strand, within GFF6576 at 17.371 kb on + strand, within GFF6576 at 17.371 kb on + strand, within GFF6576 at 17.372 kb on - strand, within GFF6576 at 17.372 kb on - strand, within GFF6576 at 17.645 kb on - strand, within GFF6576 at 17.788 kb on + strand, within GFF6576 at 17.788 kb on + strand, within GFF6576 at 17.788 kb on + strand, within GFF6576 at 17.788 kb on + strand, within GFF6576 at 17.788 kb on + strand, within GFF6576 at 17.788 kb on + strand, within GFF6576 at 17.789 kb on - strand, within GFF6576 at 17.789 kb on - strand, within GFF6576 at 17.789 kb on - strand, within GFF6576 at 17.789 kb on - strand, within GFF6576 at 18.040 kb on + strand, within GFF6576 at 18.040 kb on + strand, within GFF6576
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 16,040 + GFF6574 0.83 +1.0 16,040 + GFF6574 0.83 -0.6 16,040 + GFF6574 0.83 +0.9 16,041 - GFF6574 0.83 +0.2 16,058 + GFF6574 0.84 -1.0 16,058 + GFF6574 0.84 -0.6 16,058 + GFF6574 0.84 -0.5 16,059 - GFF6574 0.84 +0.4 16,059 - GFF6574 0.84 -0.1 16,059 - GFF6574 0.84 -1.0 16,146 + -0.3 16,147 - -0.5 16,147 - -0.2 16,147 - +0.1 16,147 - +0.5 16,147 - +0.3 16,147 - -1.4 16,217 + -0.1 16,218 - -0.5 16,218 - +0.3 16,290 - +0.4 16,313 + -0.3 16,313 + -0.3 16,313 + +0.5 16,314 - +0.7 16,314 - +0.1 16,314 - -0.3 16,668 - GFF6575 0.50 +0.4 16,668 - GFF6575 0.50 +1.8 16,668 - GFF6575 0.50 -1.4 16,679 + GFF6575 0.52 +2.7 16,680 - GFF6575 0.52 +0.8 16,718 + GFF6575 0.57 +0.7 16,849 + GFF6575 0.74 +0.5 16,849 + GFF6575 0.74 +0.4 16,850 - GFF6575 0.74 +0.0 16,850 - GFF6575 0.74 +0.2 17,300 - GFF6576 0.10 -0.2 17,300 - GFF6576 0.10 -0.7 17,302 + GFF6576 0.10 -0.1 17,302 + GFF6576 0.10 -0.5 17,302 + GFF6576 0.10 -0.2 17,302 + GFF6576 0.10 -0.3 17,302 + GFF6576 0.10 +0.8 17,302 + GFF6576 0.10 +1.3 17,302 + GFF6576 0.10 -1.3 17,302 + GFF6576 0.10 +1.3 17,302 + GFF6576 0.10 -0.5 17,302 + GFF6576 0.10 -0.4 17,303 - GFF6576 0.10 -1.1 17,303 - GFF6576 0.10 +0.7 17,303 - GFF6576 0.10 -0.3 17,303 - GFF6576 0.10 +0.7 17,303 - GFF6576 0.10 +0.4 17,303 - GFF6576 0.10 -0.9 17,303 - GFF6576 0.10 -0.5 17,303 - GFF6576 0.10 +0.1 17,305 + GFF6576 0.10 +1.0 17,305 + GFF6576 0.10 +1.0 17,305 + GFF6576 0.10 -0.5 17,306 - GFF6576 0.11 -1.7 17,306 - GFF6576 0.11 +0.3 17,306 - GFF6576 0.11 +0.9 17,306 - GFF6576 0.11 +0.7 17,371 + GFF6576 0.13 -1.4 17,371 + GFF6576 0.13 +1.4 17,371 + GFF6576 0.13 +3.0 17,371 + GFF6576 0.13 -0.5 17,372 - GFF6576 0.13 +0.3 17,372 - GFF6576 0.13 +0.1 17,645 - GFF6576 0.24 +0.2 17,788 + GFF6576 0.30 +1.0 17,788 + GFF6576 0.30 -1.6 17,788 + GFF6576 0.30 -0.3 17,788 + GFF6576 0.30 +0.3 17,788 + GFF6576 0.30 -0.9 17,788 + GFF6576 0.30 -0.1 17,789 - GFF6576 0.30 +0.2 17,789 - GFF6576 0.30 +0.6 17,789 - GFF6576 0.30 -0.9 17,789 - GFF6576 0.30 -0.1 18,040 + GFF6576 0.40 +0.3 18,040 + GFF6576 0.40 +0.6
Or see this region's nucleotide sequence