Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6374 and GFF6375 are separated by 74 nucleotides GFF6375 and GFF6376 overlap by 4 nucleotides GFF6376 and GFF6377 overlap by 23 nucleotides
GFF6374 - predicted 2-keto-3-deoxyxylonate dehydratase, at 43,606 to 44,799
GFF6374
GFF6375 - Uncharacterized aldehyde oxidase, molybdopterin-binding subunit, at 44,874 to 48,632
GFF6375
GFF6376 - Uncharacterized aldehyde oxidase, 2Fe-2S subunit, at 48,629 to 49,129
GFF6376
GFF6377 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 49,107 to 50,267
GFF6377
Position (kb) 44
45
46
47
48
49 Strain fitness (log2 ratio) -2
-1
0
1
2 at 44.376 kb on + strand, within GFF6374 at 44.825 kb on + strand at 44.825 kb on + strand at 44.825 kb on + strand at 44.826 kb on - strand at 45.311 kb on - strand, within GFF6375 at 45.428 kb on - strand, within GFF6375 at 45.598 kb on + strand, within GFF6375 at 45.761 kb on - strand, within GFF6375 at 45.767 kb on - strand, within GFF6375 at 45.811 kb on + strand, within GFF6375 at 45.833 kb on - strand, within GFF6375 at 45.935 kb on - strand, within GFF6375 at 46.197 kb on - strand, within GFF6375 at 46.501 kb on + strand, within GFF6375 at 46.502 kb on - strand, within GFF6375 at 46.807 kb on + strand, within GFF6375 at 46.807 kb on + strand, within GFF6375 at 46.808 kb on - strand, within GFF6375 at 46.808 kb on - strand, within GFF6375 at 46.958 kb on - strand, within GFF6375 at 47.014 kb on + strand, within GFF6375 at 47.116 kb on + strand, within GFF6375 at 47.117 kb on - strand, within GFF6375 at 47.156 kb on - strand, within GFF6375 at 47.419 kb on + strand, within GFF6375 at 47.620 kb on + strand, within GFF6375 at 47.620 kb on + strand, within GFF6375 at 47.620 kb on + strand, within GFF6375 at 47.621 kb on - strand, within GFF6375 at 47.621 kb on - strand, within GFF6375 at 48.058 kb on + strand, within GFF6375 at 48.133 kb on + strand, within GFF6375 at 48.133 kb on + strand, within GFF6375 at 48.134 kb on - strand, within GFF6375 at 49.136 kb on - strand at 49.136 kb on - strand at 49.136 kb on - strand at 49.136 kb on - strand at 49.136 kb on - strand at 49.184 kb on - strand at 49.306 kb on + strand, within GFF6377 at 49.306 kb on + strand, within GFF6377 at 49.307 kb on - strand, within GFF6377 at 49.307 kb on - strand, within GFF6377 at 49.325 kb on - strand, within GFF6377 at 49.366 kb on + strand, within GFF6377 at 49.366 kb on + strand, within GFF6377 at 49.496 kb on - strand, within GFF6377 at 49.496 kb on - strand, within GFF6377 at 49.545 kb on + strand, within GFF6377 at 49.546 kb on - strand, within GFF6377
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 44,376 + GFF6374 0.64 +0.1 44,825 + -0.4 44,825 + +1.4 44,825 + +1.9 44,826 - +0.9 45,311 - GFF6375 0.12 -0.8 45,428 - GFF6375 0.15 +1.5 45,598 + GFF6375 0.19 -0.0 45,761 - GFF6375 0.24 -1.2 45,767 - GFF6375 0.24 -1.4 45,811 + GFF6375 0.25 -1.0 45,833 - GFF6375 0.26 -0.5 45,935 - GFF6375 0.28 -0.3 46,197 - GFF6375 0.35 +1.1 46,501 + GFF6375 0.43 +0.4 46,502 - GFF6375 0.43 -0.2 46,807 + GFF6375 0.51 +0.2 46,807 + GFF6375 0.51 +0.5 46,808 - GFF6375 0.51 +0.1 46,808 - GFF6375 0.51 +1.4 46,958 - GFF6375 0.55 -0.3 47,014 + GFF6375 0.57 -0.8 47,116 + GFF6375 0.60 -0.4 47,117 - GFF6375 0.60 +0.6 47,156 - GFF6375 0.61 -0.3 47,419 + GFF6375 0.68 -0.1 47,620 + GFF6375 0.73 -0.3 47,620 + GFF6375 0.73 -1.3 47,620 + GFF6375 0.73 +2.2 47,621 - GFF6375 0.73 -0.6 47,621 - GFF6375 0.73 -1.7 48,058 + GFF6375 0.85 -0.9 48,133 + GFF6375 0.87 -0.7 48,133 + GFF6375 0.87 -0.3 48,134 - GFF6375 0.87 -0.6 49,136 - +0.5 49,136 - -0.1 49,136 - -0.0 49,136 - +0.5 49,136 - -0.9 49,184 - +0.4 49,306 + GFF6377 0.17 +0.4 49,306 + GFF6377 0.17 +0.5 49,307 - GFF6377 0.17 +0.4 49,307 - GFF6377 0.17 -0.2 49,325 - GFF6377 0.19 -2.1 49,366 + GFF6377 0.22 +0.4 49,366 + GFF6377 0.22 +0.2 49,496 - GFF6377 0.34 -1.7 49,496 - GFF6377 0.34 -0.6 49,545 + GFF6377 0.38 -0.9 49,546 - GFF6377 0.38 +0.1
Or see this region's nucleotide sequence