Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6357 and GFF6358 overlap by 8 nucleotides GFF6358 and GFF6359 are separated by 22 nucleotides GFF6359 and GFF6360 are separated by 17 nucleotides GFF6360 and GFF6361 are separated by 35 nucleotides
GFF6357 - T6SS forkhead associated domain protein ImpI/VasC, at 22,098 to 23,984
GFF6357
GFF6358 - T6SS protein serine/threonine phosphatase PppA, at 23,977 to 24,798
GFF6358
GFF6359 - FIG01219766: hypothetical protein, at 24,821 to 25,201
GFF6359
GFF6360 - FIG00432660: hypothetical protein, at 25,219 to 25,884
GFF6360
GFF6361 - Pentapeptide repeat family protein, at 25,920 to 26,990
GFF6361
Position (kb) 24
25
26 Strain fitness (log2 ratio) -1
0
1 at 23.940 kb on + strand at 23.940 kb on + strand at 23.940 kb on + strand at 23.941 kb on - strand at 24.379 kb on + strand, within GFF6358 at 24.721 kb on + strand at 24.721 kb on + strand at 24.722 kb on - strand at 24.833 kb on + strand at 24.834 kb on - strand at 24.834 kb on - strand at 25.071 kb on + strand, within GFF6359 at 25.071 kb on + strand, within GFF6359 at 25.071 kb on + strand, within GFF6359 at 25.071 kb on + strand, within GFF6359 at 25.072 kb on - strand, within GFF6359 at 25.072 kb on - strand, within GFF6359 at 25.072 kb on - strand, within GFF6359 at 25.072 kb on - strand, within GFF6359 at 25.072 kb on - strand, within GFF6359 at 25.072 kb on - strand, within GFF6359 at 25.151 kb on + strand, within GFF6359 at 25.556 kb on - strand, within GFF6360 at 25.590 kb on + strand, within GFF6360
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 23,940 + +0.3 23,940 + +0.0 23,940 + -0.0 23,941 - -0.1 24,379 + GFF6358 0.49 +0.5 24,721 + -1.3 24,721 + +0.0 24,722 - +0.1 24,833 + -1.4 24,834 - +0.4 24,834 - -0.1 25,071 + GFF6359 0.66 -0.2 25,071 + GFF6359 0.66 -0.7 25,071 + GFF6359 0.66 -1.6 25,071 + GFF6359 0.66 +0.2 25,072 - GFF6359 0.66 -0.0 25,072 - GFF6359 0.66 +1.1 25,072 - GFF6359 0.66 -0.5 25,072 - GFF6359 0.66 +1.0 25,072 - GFF6359 0.66 +0.4 25,072 - GFF6359 0.66 +0.0 25,151 + GFF6359 0.87 -0.5 25,556 - GFF6360 0.51 -0.3 25,590 + GFF6360 0.56 -0.1
Or see this region's nucleotide sequence