Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF629 and GFF630 are separated by 6 nucleotides GFF630 and GFF631 are separated by 16 nucleotides GFF631 and GFF632 are separated by 24 nucleotides GFF632 and GFF633 are separated by 1 nucleotides
GFF629 - hypothetical protein, at 678,524 to 679,903
GFF629
GFF630 - CxxC_CXXC_SSSS domain protein, FmdB family, at 679,910 to 680,254
GFF630
GFF631 - Formamidase (EC 3.5.1.49), at 680,271 to 681,503
GFF631
GFF632 - Urea ABC transporter, ATPase protein UrtE, at 681,528 to 682,229
GFF632
GFF633 - Urea ABC transporter, ATPase protein UrtD, at 682,231 to 682,974
GFF633
Position (kb) 680
681
682 Strain fitness (log2 ratio) -1
0
1 at 680.244 kb on + strand at 680.244 kb on + strand at 680.245 kb on - strand at 680.474 kb on - strand, within GFF631 at 680.599 kb on + strand, within GFF631 at 680.599 kb on + strand, within GFF631 at 680.600 kb on - strand, within GFF631 at 680.600 kb on - strand, within GFF631 at 680.600 kb on - strand, within GFF631 at 680.647 kb on + strand, within GFF631 at 680.648 kb on - strand, within GFF631 at 680.648 kb on - strand, within GFF631 at 680.785 kb on + strand, within GFF631 at 680.785 kb on + strand, within GFF631 at 681.541 kb on + strand at 681.979 kb on + strand, within GFF632 at 681.979 kb on + strand, within GFF632
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 680,244 + +0.6 680,244 + -0.7 680,245 - +0.9 680,474 - GFF631 0.16 -0.2 680,599 + GFF631 0.27 +0.7 680,599 + GFF631 0.27 +1.5 680,600 - GFF631 0.27 +0.4 680,600 - GFF631 0.27 -0.1 680,600 - GFF631 0.27 +0.2 680,647 + GFF631 0.30 -0.2 680,648 - GFF631 0.31 -0.0 680,648 - GFF631 0.31 -0.3 680,785 + GFF631 0.42 +1.3 680,785 + GFF631 0.42 -0.3 681,541 + -0.2 681,979 + GFF632 0.64 -0.1 681,979 + GFF632 0.64 -0.4
Or see this region's nucleotide sequence