Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF61 and GFF62 are separated by 16 nucleotides GFF62 and GFF63 are separated by 7 nucleotides
GFF61 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 76,935 to 78,059
GFF61
GFF62 - hypothetical protein, at 78,076 to 79,422
GFF62
GFF63 - Putative glycosyltransferase, at 79,430 to 80,512
GFF63
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 77.077 kb on - strand, within GFF61 at 77.187 kb on + strand, within GFF61 at 77.188 kb on - strand, within GFF61 at 77.322 kb on + strand, within GFF61 at 77.323 kb on - strand, within GFF61 at 77.323 kb on - strand, within GFF61 at 77.347 kb on - strand, within GFF61 at 77.392 kb on - strand, within GFF61 at 77.424 kb on + strand, within GFF61 at 77.424 kb on + strand, within GFF61 at 77.448 kb on + strand, within GFF61 at 77.449 kb on - strand, within GFF61 at 77.460 kb on + strand, within GFF61 at 77.461 kb on - strand, within GFF61 at 77.461 kb on - strand, within GFF61 at 77.461 kb on - strand, within GFF61 at 77.469 kb on + strand, within GFF61 at 77.470 kb on - strand, within GFF61 at 77.634 kb on + strand, within GFF61 at 77.634 kb on + strand, within GFF61 at 77.682 kb on + strand, within GFF61 at 77.683 kb on - strand, within GFF61 at 77.683 kb on - strand, within GFF61 at 77.706 kb on + strand, within GFF61 at 78.247 kb on + strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 78.356 kb on - strand, within GFF62 at 79.114 kb on + strand, within GFF62 at 79.114 kb on + strand, within GFF62 at 79.252 kb on + strand, within GFF62 at 79.253 kb on - strand, within GFF62 at 79.439 kb on + strand at 79.439 kb on + strand at 79.439 kb on + strand at 79.440 kb on - strand at 79.440 kb on - strand at 79.440 kb on - strand at 79.448 kb on + strand at 79.449 kb on - strand at 79.449 kb on - strand at 79.574 kb on + strand, within GFF63 at 79.574 kb on + strand, within GFF63 at 79.575 kb on - strand, within GFF63 at 79.575 kb on - strand, within GFF63 at 79.628 kb on + strand, within GFF63 at 79.628 kb on + strand, within GFF63 at 79.629 kb on - strand, within GFF63 at 79.629 kb on - strand, within GFF63 at 79.629 kb on - strand, within GFF63 at 79.637 kb on + strand, within GFF63 at 79.673 kb on + strand, within GFF63 at 79.673 kb on + strand, within GFF63 at 79.691 kb on + strand, within GFF63 at 79.757 kb on + strand, within GFF63 at 79.757 kb on + strand, within GFF63 at 79.757 kb on + strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.758 kb on - strand, within GFF63 at 79.766 kb on + strand, within GFF63 at 79.776 kb on - strand, within GFF63 at 80.000 kb on + strand, within GFF63 at 80.006 kb on + strand, within GFF63 at 80.006 kb on + strand, within GFF63 at 80.006 kb on + strand, within GFF63 at 80.007 kb on - strand, within GFF63 at 80.007 kb on - strand, within GFF63 at 80.202 kb on - strand, within GFF63 at 80.202 kb on - strand, within GFF63 at 80.369 kb on + strand, within GFF63 at 80.369 kb on + strand, within GFF63 at 80.370 kb on - strand, within GFF63 at 80.384 kb on + strand, within GFF63
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 77,077 - GFF61 0.13 -0.5 77,187 + GFF61 0.22 +2.0 77,188 - GFF61 0.22 +0.3 77,322 + GFF61 0.34 -0.3 77,323 - GFF61 0.34 +0.7 77,323 - GFF61 0.34 -2.8 77,347 - GFF61 0.37 +0.6 77,392 - GFF61 0.41 +0.5 77,424 + GFF61 0.43 +0.8 77,424 + GFF61 0.43 -1.2 77,448 + GFF61 0.46 +0.3 77,449 - GFF61 0.46 +0.1 77,460 + GFF61 0.47 -0.8 77,461 - GFF61 0.47 -0.5 77,461 - GFF61 0.47 -0.1 77,461 - GFF61 0.47 -1.3 77,469 + GFF61 0.47 +0.0 77,470 - GFF61 0.48 -1.4 77,634 + GFF61 0.62 -0.1 77,634 + GFF61 0.62 +0.2 77,682 + GFF61 0.66 -0.1 77,683 - GFF61 0.66 -0.5 77,683 - GFF61 0.66 +0.0 77,706 + GFF61 0.69 +0.6 78,247 + GFF62 0.13 -1.0 78,356 - GFF62 0.21 +0.4 78,356 - GFF62 0.21 +0.7 78,356 - GFF62 0.21 -0.7 78,356 - GFF62 0.21 -0.9 78,356 - GFF62 0.21 -0.7 78,356 - GFF62 0.21 -1.1 79,114 + GFF62 0.77 -2.3 79,114 + GFF62 0.77 -0.7 79,252 + GFF62 0.87 -1.5 79,253 - GFF62 0.87 +0.3 79,439 + -0.3 79,439 + -0.1 79,439 + +0.5 79,440 - -0.7 79,440 - -0.0 79,440 - +0.4 79,448 + -0.4 79,449 - -0.9 79,449 - +0.1 79,574 + GFF63 0.13 -0.5 79,574 + GFF63 0.13 -0.5 79,575 - GFF63 0.13 -0.3 79,575 - GFF63 0.13 +0.1 79,628 + GFF63 0.18 -1.0 79,628 + GFF63 0.18 +0.3 79,629 - GFF63 0.18 -0.3 79,629 - GFF63 0.18 -0.7 79,629 - GFF63 0.18 +0.2 79,637 + GFF63 0.19 -0.6 79,673 + GFF63 0.22 +1.3 79,673 + GFF63 0.22 -1.0 79,691 + GFF63 0.24 -1.2 79,757 + GFF63 0.30 +1.0 79,757 + GFF63 0.30 +0.2 79,757 + GFF63 0.30 -0.6 79,758 - GFF63 0.30 -0.1 79,758 - GFF63 0.30 +1.6 79,758 - GFF63 0.30 -0.3 79,758 - GFF63 0.30 -0.7 79,758 - GFF63 0.30 -1.2 79,758 - GFF63 0.30 -0.7 79,766 + GFF63 0.31 -1.2 79,776 - GFF63 0.32 -0.2 80,000 + GFF63 0.53 +0.3 80,006 + GFF63 0.53 -0.7 80,006 + GFF63 0.53 -0.5 80,006 + GFF63 0.53 -0.7 80,007 - GFF63 0.53 +0.2 80,007 - GFF63 0.53 -2.2 80,202 - GFF63 0.71 -1.2 80,202 - GFF63 0.71 -0.3 80,369 + GFF63 0.87 +0.8 80,369 + GFF63 0.87 -1.5 80,370 - GFF63 0.87 -0.5 80,384 + GFF63 0.88 -0.0
Or see this region's nucleotide sequence