Strain Fitness in Variovorax sp. SCN45 around GFF6144

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6143 and GFF6144 are separated by 178 nucleotidesGFF6144 and GFF6145 are separated by 155 nucleotidesGFF6145 and GFF6146 are separated by 1 nucleotides GFF6143 - 2-aminoethylphosphonate ABC transporter ATP-binding protein (TC 3.A.1.9.1), at 47,020 to 48,156 GFF6143 GFF6144 - 2-aminoethylphosphonate ABC transporter substrate-binding protein, at 48,335 to 49,375 GFF6144 GFF6145 - 2-aminoethylphosphonate utilization transcriptional regulator, GntR family, at 49,531 to 50,259 GFF6145 GFF6146 - Phosphonoacetate hydrolase (EC 3.11.1.2), at 50,261 to 51,487 GFF6146 Position (kb) 48 49 50Strain fitness (log2 ratio) -2 -1 0 1at 47.441 kb on + strand, within GFF6143at 47.441 kb on + strand, within GFF6143at 47.442 kb on - strand, within GFF6143at 47.717 kb on + strand, within GFF6143at 47.718 kb on - strand, within GFF6143at 47.718 kb on - strand, within GFF6143at 47.855 kb on + strand, within GFF6143at 47.855 kb on + strand, within GFF6143at 48.151 kb on - strandat 48.336 kb on + strandat 48.336 kb on + strandat 48.441 kb on + strand, within GFF6144at 48.441 kb on + strand, within GFF6144at 48.441 kb on + strand, within GFF6144at 48.442 kb on - strand, within GFF6144at 48.442 kb on - strand, within GFF6144at 48.483 kb on + strand, within GFF6144at 48.750 kb on + strand, within GFF6144at 48.751 kb on - strand, within GFF6144at 48.751 kb on - strand, within GFF6144at 48.783 kb on + strand, within GFF6144at 48.783 kb on + strand, within GFF6144at 48.783 kb on + strand, within GFF6144at 48.784 kb on - strand, within GFF6144at 48.784 kb on - strand, within GFF6144at 48.784 kb on - strand, within GFF6144at 48.784 kb on - strand, within GFF6144at 48.837 kb on + strand, within GFF6144at 48.838 kb on - strand, within GFF6144at 48.885 kb on + strand, within GFF6144at 48.885 kb on + strand, within GFF6144at 48.885 kb on + strand, within GFF6144at 48.885 kb on + strand, within GFF6144at 48.886 kb on - strand, within GFF6144at 49.020 kb on + strand, within GFF6144at 49.020 kb on + strand, within GFF6144at 49.020 kb on + strand, within GFF6144at 49.057 kb on - strand, within GFF6144at 49.233 kb on + strand, within GFF6144at 49.234 kb on - strand, within GFF6144at 49.266 kb on + strand, within GFF6144at 49.266 kb on + strand, within GFF6144at 49.267 kb on - strand, within GFF6144at 49.524 kb on - strandat 49.524 kb on - strandat 49.524 kb on - strandat 49.804 kb on + strand, within GFF6145at 49.805 kb on - strand, within GFF6145at 49.939 kb on + strand, within GFF6145at 49.939 kb on + strand, within GFF6145at 49.940 kb on - strand, within GFF6145at 50.011 kb on + strand, within GFF6145at 50.011 kb on + strand, within GFF6145at 50.012 kb on - strand, within GFF6145at 50.012 kb on - strand, within GFF6145

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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47,441 + GFF6143 0.37 +0.2
47,441 + GFF6143 0.37 +1.5
47,442 - GFF6143 0.37 -0.3
47,717 + GFF6143 0.61 -0.2
47,718 - GFF6143 0.61 -0.2
47,718 - GFF6143 0.61 -0.4
47,855 + GFF6143 0.73 -0.7
47,855 + GFF6143 0.73 +1.0
48,151 - -0.4
48,336 + -0.6
48,336 + -0.5
48,441 + GFF6144 0.10 -2.7
48,441 + GFF6144 0.10 +0.2
48,441 + GFF6144 0.10 -0.1
48,442 - GFF6144 0.10 -0.1
48,442 - GFF6144 0.10 +0.2
48,483 + GFF6144 0.14 -0.1
48,750 + GFF6144 0.40 +1.5
48,751 - GFF6144 0.40 -0.2
48,751 - GFF6144 0.40 -0.1
48,783 + GFF6144 0.43 -0.1
48,783 + GFF6144 0.43 -1.1
48,783 + GFF6144 0.43 -0.4
48,784 - GFF6144 0.43 -1.8
48,784 - GFF6144 0.43 +0.0
48,784 - GFF6144 0.43 -0.8
48,784 - GFF6144 0.43 -0.1
48,837 + GFF6144 0.48 -0.6
48,838 - GFF6144 0.48 -1.3
48,885 + GFF6144 0.53 +0.8
48,885 + GFF6144 0.53 +0.3
48,885 + GFF6144 0.53 +0.3
48,885 + GFF6144 0.53 -0.7
48,886 - GFF6144 0.53 -0.2
49,020 + GFF6144 0.66 -0.3
49,020 + GFF6144 0.66 -0.7
49,020 + GFF6144 0.66 +0.5
49,057 - GFF6144 0.69 +0.1
49,233 + GFF6144 0.86 -0.2
49,234 - GFF6144 0.86 -0.5
49,266 + GFF6144 0.89 +0.5
49,266 + GFF6144 0.89 -0.3
49,267 - GFF6144 0.90 +0.9
49,524 - -1.5
49,524 - -0.1
49,524 - +0.8
49,804 + GFF6145 0.37 -1.4
49,805 - GFF6145 0.38 -2.2
49,939 + GFF6145 0.56 -0.5
49,939 + GFF6145 0.56 -1.1
49,940 - GFF6145 0.56 -0.9
50,011 + GFF6145 0.66 -0.2
50,011 + GFF6145 0.66 -0.3
50,012 - GFF6145 0.66 -0.5
50,012 - GFF6145 0.66 +0.2

Or see this region's nucleotide sequence