Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6060 and GFF6061 overlap by 4 nucleotides GFF6061 and GFF6062 are separated by 64 nucleotides GFF6062 and GFF6063 are separated by 22 nucleotides
GFF6060 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 121,544 to 122,608
GFF6060
GFF6061 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 122,605 to 123,468
GFF6061
GFF6062 - Sodium-dependent phosphate transporter, at 123,533 to 125,194
GFF6062
GFF6063 - Sodium/bile acid symporter family, at 125,217 to 126,221
GFF6063
Position (kb) 123
124
125
126 Strain fitness (log2 ratio) -2
-1
0
1 at 122.689 kb on + strand at 122.689 kb on + strand at 122.690 kb on - strand at 122.690 kb on - strand at 122.713 kb on + strand, within GFF6061 at 122.812 kb on + strand, within GFF6061 at 122.813 kb on - strand, within GFF6061 at 122.857 kb on + strand, within GFF6061 at 122.857 kb on + strand, within GFF6061 at 122.857 kb on + strand, within GFF6061 at 122.920 kb on + strand, within GFF6061 at 122.920 kb on + strand, within GFF6061 at 122.920 kb on + strand, within GFF6061 at 122.921 kb on - strand, within GFF6061 at 122.921 kb on - strand, within GFF6061 at 122.921 kb on - strand, within GFF6061 at 123.417 kb on + strand at 123.518 kb on + strand at 123.543 kb on + strand at 123.543 kb on + strand at 123.543 kb on + strand at 123.543 kb on + strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.544 kb on - strand at 123.583 kb on + strand at 123.598 kb on + strand at 123.615 kb on + strand at 123.616 kb on - strand at 123.822 kb on - strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.319 kb on + strand, within GFF6062 at 124.320 kb on - strand, within GFF6062 at 124.320 kb on - strand, within GFF6062 at 124.667 kb on + strand, within GFF6062 at 124.667 kb on + strand, within GFF6062 at 124.668 kb on - strand, within GFF6062 at 124.668 kb on - strand, within GFF6062 at 124.668 kb on - strand, within GFF6062 at 124.682 kb on + strand, within GFF6062 at 124.682 kb on + strand, within GFF6062 at 124.682 kb on + strand, within GFF6062 at 124.683 kb on - strand, within GFF6062 at 124.683 kb on - strand, within GFF6062 at 124.683 kb on - strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.685 kb on + strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 124.686 kb on - strand, within GFF6062 at 125.175 kb on + strand at 125.175 kb on + strand at 125.175 kb on + strand at 125.175 kb on + strand at 125.175 kb on + strand at 125.176 kb on - strand at 125.218 kb on + strand at 125.218 kb on + strand at 125.218 kb on + strand at 125.219 kb on - strand at 125.219 kb on - strand at 125.272 kb on + strand at 125.273 kb on - strand at 125.273 kb on - strand at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.587 kb on + strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.588 kb on - strand, within GFF6063 at 125.737 kb on + strand, within GFF6063 at 125.923 kb on + strand, within GFF6063 at 125.923 kb on + strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063 at 125.924 kb on - strand, within GFF6063
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 122,689 + +0.1 122,689 + -1.0 122,690 - -0.3 122,690 - -0.4 122,713 + GFF6061 0.12 -1.5 122,812 + GFF6061 0.24 +0.2 122,813 - GFF6061 0.24 -0.4 122,857 + GFF6061 0.29 -1.2 122,857 + GFF6061 0.29 +1.0 122,857 + GFF6061 0.29 -0.1 122,920 + GFF6061 0.36 +0.2 122,920 + GFF6061 0.36 +0.5 122,920 + GFF6061 0.36 -1.3 122,921 - GFF6061 0.37 -0.7 122,921 - GFF6061 0.37 -0.3 122,921 - GFF6061 0.37 +0.3 123,417 + -0.4 123,518 + -1.1 123,543 + -2.3 123,543 + -0.7 123,543 + +0.2 123,543 + -0.5 123,544 - -0.5 123,544 - -1.6 123,544 - +0.8 123,544 - -0.3 123,544 - -0.2 123,544 - +0.0 123,544 - -0.4 123,583 + +1.6 123,598 + -0.8 123,615 + -0.8 123,616 - -0.3 123,822 - GFF6062 0.17 +0.0 124,319 + GFF6062 0.47 -1.5 124,319 + GFF6062 0.47 +1.1 124,319 + GFF6062 0.47 +0.0 124,319 + GFF6062 0.47 +0.9 124,320 - GFF6062 0.47 +0.3 124,320 - GFF6062 0.47 -0.2 124,667 + GFF6062 0.68 +1.1 124,667 + GFF6062 0.68 -0.5 124,668 - GFF6062 0.68 -0.1 124,668 - GFF6062 0.68 -0.0 124,668 - GFF6062 0.68 -1.2 124,682 + GFF6062 0.69 -0.2 124,682 + GFF6062 0.69 -0.3 124,682 + GFF6062 0.69 +0.6 124,683 - GFF6062 0.69 +0.9 124,683 - GFF6062 0.69 +0.4 124,683 - GFF6062 0.69 -0.7 124,685 + GFF6062 0.69 -0.7 124,685 + GFF6062 0.69 +1.3 124,685 + GFF6062 0.69 -0.1 124,685 + GFF6062 0.69 -0.3 124,685 + GFF6062 0.69 +0.5 124,685 + GFF6062 0.69 +0.9 124,686 - GFF6062 0.69 -0.2 124,686 - GFF6062 0.69 +0.0 124,686 - GFF6062 0.69 +0.3 124,686 - GFF6062 0.69 +0.1 124,686 - GFF6062 0.69 +1.3 124,686 - GFF6062 0.69 -0.1 125,175 + -1.0 125,175 + -0.7 125,175 + -0.1 125,175 + -0.7 125,175 + +0.3 125,176 - -0.3 125,218 + -1.7 125,218 + -0.0 125,218 + +1.1 125,219 - +0.3 125,219 - -0.4 125,272 + -0.4 125,273 - +0.8 125,273 - +0.6 125,587 + GFF6063 0.37 -0.9 125,587 + GFF6063 0.37 -0.7 125,587 + GFF6063 0.37 +0.0 125,587 + GFF6063 0.37 -0.6 125,587 + GFF6063 0.37 -0.9 125,587 + GFF6063 0.37 +1.5 125,587 + GFF6063 0.37 -0.3 125,588 - GFF6063 0.37 +1.0 125,588 - GFF6063 0.37 +0.0 125,588 - GFF6063 0.37 -0.2 125,588 - GFF6063 0.37 +0.3 125,588 - GFF6063 0.37 +0.2 125,737 + GFF6063 0.52 -0.2 125,923 + GFF6063 0.70 +0.5 125,923 + GFF6063 0.70 -0.1 125,924 - GFF6063 0.70 -0.3 125,924 - GFF6063 0.70 +0.5 125,924 - GFF6063 0.70 +0.9 125,924 - GFF6063 0.70 -0.7 125,924 - GFF6063 0.70 +1.4 125,924 - GFF6063 0.70 +0.3 125,924 - GFF6063 0.70 -0.3
Or see this region's nucleotide sequence