Strain Fitness in Variovorax sp. SCN45 around GFF605

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF604 and GFF605 are separated by 28 nucleotidesGFF605 and GFF606 are separated by 34 nucleotides GFF604 - Tricarballylate utilization protein, TcuB, at 649,399 to 650,619 GFF604 GFF605 - Aldolase related to tricarballylate utilization, at 650,648 to 651,436 GFF605 GFF606 - BUG/TctC family periplasmic protein, at 651,471 to 652,454 GFF606 Position (kb) 650 651 652Strain fitness (log2 ratio) -3 -2 -1 0 1at 649.672 kb on + strand, within GFF604at 649.673 kb on - strand, within GFF604at 649.738 kb on + strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.739 kb on - strand, within GFF604at 649.747 kb on + strand, within GFF604at 649.747 kb on + strand, within GFF604at 649.748 kb on - strand, within GFF604at 649.895 kb on - strand, within GFF604at 649.895 kb on - strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.089 kb on + strand, within GFF604at 650.090 kb on - strand, within GFF604at 650.090 kb on - strand, within GFF604at 650.177 kb on - strand, within GFF604at 650.177 kb on - strand, within GFF604at 650.177 kb on - strand, within GFF604at 650.221 kb on + strand, within GFF604at 650.221 kb on + strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.222 kb on - strand, within GFF604at 650.228 kb on - strand, within GFF604at 650.228 kb on - strand, within GFF604at 650.252 kb on - strand, within GFF604at 650.664 kb on + strandat 650.664 kb on + strandat 650.800 kb on - strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.846 kb on + strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.847 kb on - strand, within GFF605at 650.931 kb on - strand, within GFF605at 650.951 kb on + strand, within GFF605at 651.099 kb on - strand, within GFF605at 651.107 kb on + strand, within GFF605at 651.107 kb on + strand, within GFF605at 651.344 kb on + strand, within GFF605at 651.344 kb on + strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.345 kb on - strand, within GFF605at 651.405 kb on + strandat 651.406 kb on - strandat 651.750 kb on + strand, within GFF606at 651.801 kb on + strand, within GFF606at 651.801 kb on + strand, within GFF606at 651.801 kb on + strand, within GFF606at 651.802 kb on - strand, within GFF606at 651.802 kb on - strand, within GFF606at 651.816 kb on + strand, within GFF606at 651.816 kb on + strand, within GFF606at 651.817 kb on - strand, within GFF606at 651.892 kb on + strand, within GFF606at 651.893 kb on - strand, within GFF606at 651.893 kb on - strand, within GFF606at 651.924 kb on + strand, within GFF606at 651.955 kb on - strand, within GFF606at 651.955 kb on - strand, within GFF606at 651.996 kb on + strand, within GFF606at 651.996 kb on + strand, within GFF606at 651.996 kb on + strand, within GFF606at 651.996 kb on + strand, within GFF606at 651.996 kb on + strand, within GFF606at 651.996 kb on + strand, within GFF606at 651.997 kb on - strand, within GFF606at 651.997 kb on - strand, within GFF606at 651.997 kb on - strand, within GFF606at 652.041 kb on + strand, within GFF606at 652.041 kb on + strand, within GFF606at 652.041 kb on + strand, within GFF606at 652.042 kb on - strand, within GFF606at 652.042 kb on - strand, within GFF606at 652.042 kb on - strand, within GFF606at 652.309 kb on - strand, within GFF606at 652.316 kb on - strand, within GFF606at 652.316 kb on - strand, within GFF606at 652.404 kb on + strandat 652.404 kb on + strandat 652.404 kb on + strandat 652.404 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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649,672 + GFF604 0.22 -0.1
649,673 - GFF604 0.22 -0.9
649,738 + GFF604 0.28 -0.3
649,739 - GFF604 0.28 +0.5
649,739 - GFF604 0.28 -1.6
649,739 - GFF604 0.28 -0.2
649,739 - GFF604 0.28 +0.0
649,739 - GFF604 0.28 +0.1
649,747 + GFF604 0.29 +0.0
649,747 + GFF604 0.29 +0.3
649,748 - GFF604 0.29 +0.0
649,895 - GFF604 0.41 +0.7
649,895 - GFF604 0.41 -0.4
650,089 + GFF604 0.57 -0.2
650,089 + GFF604 0.57 +0.2
650,089 + GFF604 0.57 +0.3
650,089 + GFF604 0.57 -0.1
650,090 - GFF604 0.57 -0.5
650,090 - GFF604 0.57 -1.5
650,177 - GFF604 0.64 +0.1
650,177 - GFF604 0.64 -0.1
650,177 - GFF604 0.64 -1.1
650,221 + GFF604 0.67 -0.5
650,221 + GFF604 0.67 -0.3
650,222 - GFF604 0.67 -0.5
650,222 - GFF604 0.67 +0.5
650,222 - GFF604 0.67 -0.4
650,222 - GFF604 0.67 -0.3
650,222 - GFF604 0.67 -0.5
650,228 - GFF604 0.68 -0.1
650,228 - GFF604 0.68 -0.5
650,252 - GFF604 0.70 -0.7
650,664 + -0.2
650,664 + -0.3
650,800 - GFF605 0.19 -1.1
650,846 + GFF605 0.25 -0.5
650,846 + GFF605 0.25 -1.0
650,846 + GFF605 0.25 -1.5
650,846 + GFF605 0.25 -0.5
650,847 - GFF605 0.25 -0.3
650,847 - GFF605 0.25 +0.3
650,847 - GFF605 0.25 -0.5
650,847 - GFF605 0.25 -0.6
650,931 - GFF605 0.36 +0.4
650,951 + GFF605 0.38 -2.1
651,099 - GFF605 0.57 -0.4
651,107 + GFF605 0.58 +1.0
651,107 + GFF605 0.58 -0.4
651,344 + GFF605 0.88 +0.3
651,344 + GFF605 0.88 +0.0
651,345 - GFF605 0.88 -0.5
651,345 - GFF605 0.88 -0.6
651,345 - GFF605 0.88 -0.9
651,345 - GFF605 0.88 -1.2
651,345 - GFF605 0.88 -0.6
651,405 + -0.4
651,406 - -1.0
651,750 + GFF606 0.28 +0.3
651,801 + GFF606 0.34 +0.0
651,801 + GFF606 0.34 +0.1
651,801 + GFF606 0.34 +1.3
651,802 - GFF606 0.34 +0.4
651,802 - GFF606 0.34 +0.1
651,816 + GFF606 0.35 -0.9
651,816 + GFF606 0.35 -1.6
651,817 - GFF606 0.35 -0.2
651,892 + GFF606 0.43 -3.1
651,893 - GFF606 0.43 +0.1
651,893 - GFF606 0.43 -1.6
651,924 + GFF606 0.46 -0.7
651,955 - GFF606 0.49 -0.3
651,955 - GFF606 0.49 -1.6
651,996 + GFF606 0.53 -0.2
651,996 + GFF606 0.53 -0.7
651,996 + GFF606 0.53 +0.7
651,996 + GFF606 0.53 +0.9
651,996 + GFF606 0.53 +0.7
651,996 + GFF606 0.53 +0.3
651,997 - GFF606 0.53 +0.0
651,997 - GFF606 0.53 +0.4
651,997 - GFF606 0.53 +0.4
652,041 + GFF606 0.58 +1.3
652,041 + GFF606 0.58 +0.3
652,041 + GFF606 0.58 -0.7
652,042 - GFF606 0.58 +0.3
652,042 - GFF606 0.58 +0.0
652,042 - GFF606 0.58 -0.4
652,309 - GFF606 0.85 -0.6
652,316 - GFF606 0.86 -0.3
652,316 - GFF606 0.86 -2.1
652,404 + +0.1
652,404 + +0.3
652,404 + +0.8
652,404 + -0.3

Or see this region's nucleotide sequence