Strain Fitness in Variovorax sp. SCN45 around GFF5946

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5945 and GFF5946 are separated by 60 nucleotides GFF5945 - Membrane-bound lytic murein transglycosylase A, at 166,195 to 167,796 GFF5945 GFF5946 - T6SS Serine/threonine protein kinase (EC 2.7.11.1) PpkA, at 167,857 to 169,785 GFF5946 Position (kb) 167 168 169 170Strain fitness (log2 ratio) -2 -1 0 1 2at 166.892 kb on + strand, within GFF5945at 167.036 kb on + strand, within GFF5945at 167.037 kb on - strand, within GFF5945at 167.037 kb on - strand, within GFF5945at 167.039 kb on + strand, within GFF5945at 167.039 kb on + strand, within GFF5945at 167.265 kb on - strand, within GFF5945at 167.265 kb on - strand, within GFF5945at 167.265 kb on - strand, within GFF5945at 167.325 kb on - strand, within GFF5945at 167.372 kb on + strand, within GFF5945at 167.372 kb on + strand, within GFF5945at 167.570 kb on + strand, within GFF5945at 167.570 kb on + strand, within GFF5945at 167.571 kb on - strand, within GFF5945at 167.571 kb on - strand, within GFF5945at 167.595 kb on + strand, within GFF5945at 167.595 kb on + strand, within GFF5945at 167.596 kb on - strand, within GFF5945at 167.858 kb on + strandat 167.858 kb on + strandat 167.859 kb on - strandat 167.859 kb on - strandat 167.859 kb on - strandat 167.891 kb on + strandat 167.892 kb on - strandat 167.892 kb on - strandat 168.162 kb on - strand, within GFF5946at 168.162 kb on - strand, within GFF5946at 168.162 kb on - strand, within GFF5946at 168.162 kb on - strand, within GFF5946at 168.162 kb on - strand, within GFF5946at 168.224 kb on + strand, within GFF5946at 168.224 kb on + strand, within GFF5946at 168.225 kb on - strand, within GFF5946at 168.225 kb on - strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.914 kb on + strand, within GFF5946at 168.915 kb on - strand, within GFF5946at 168.915 kb on - strand, within GFF5946at 168.915 kb on - strand, within GFF5946at 168.915 kb on - strand, within GFF5946at 168.915 kb on - strand, within GFF5946at 168.996 kb on - strand, within GFF5946at 169.040 kb on + strand, within GFF5946at 169.040 kb on + strand, within GFF5946at 169.193 kb on + strand, within GFF5946at 169.194 kb on - strand, within GFF5946at 169.194 kb on - strand, within GFF5946at 169.316 kb on + strand, within GFF5946at 169.316 kb on + strand, within GFF5946at 169.317 kb on - strand, within GFF5946at 169.317 kb on - strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.334 kb on + strand, within GFF5946at 169.335 kb on - strand, within GFF5946at 169.335 kb on - strand, within GFF5946at 169.335 kb on - strand, within GFF5946at 169.335 kb on - strand, within GFF5946at 169.335 kb on - strand, within GFF5946at 169.335 kb on - strand, within GFF5946at 169.365 kb on - strand, within GFF5946at 169.517 kb on + strand, within GFF5946at 169.517 kb on + strand, within GFF5946at 169.518 kb on - strand, within GFF5946at 169.518 kb on - strand, within GFF5946at 169.518 kb on - strand, within GFF5946at 169.565 kb on + strand, within GFF5946at 169.566 kb on - strand, within GFF5946at 169.591 kb on + strand, within GFF5946at 169.631 kb on - strandat 169.753 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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166,892 + GFF5945 0.44 -0.5
167,036 + GFF5945 0.52 -0.2
167,037 - GFF5945 0.53 -0.3
167,037 - GFF5945 0.53 +0.3
167,039 + GFF5945 0.53 -0.0
167,039 + GFF5945 0.53 +0.4
167,265 - GFF5945 0.67 -1.4
167,265 - GFF5945 0.67 -2.0
167,265 - GFF5945 0.67 -0.1
167,325 - GFF5945 0.71 -0.5
167,372 + GFF5945 0.73 +0.4
167,372 + GFF5945 0.73 +1.2
167,570 + GFF5945 0.86 +0.2
167,570 + GFF5945 0.86 +0.8
167,571 - GFF5945 0.86 -1.1
167,571 - GFF5945 0.86 +0.5
167,595 + GFF5945 0.87 +0.0
167,595 + GFF5945 0.87 -1.4
167,596 - GFF5945 0.87 +0.2
167,858 + -0.8
167,858 + -0.2
167,859 - +1.8
167,859 - -0.0
167,859 - +0.0
167,891 + -0.6
167,892 - -0.7
167,892 - -0.6
168,162 - GFF5946 0.16 -0.5
168,162 - GFF5946 0.16 -0.5
168,162 - GFF5946 0.16 -0.2
168,162 - GFF5946 0.16 +0.0
168,162 - GFF5946 0.16 -1.2
168,224 + GFF5946 0.19 +0.8
168,224 + GFF5946 0.19 +0.1
168,225 - GFF5946 0.19 -0.2
168,225 - GFF5946 0.19 -1.0
168,914 + GFF5946 0.55 -0.2
168,914 + GFF5946 0.55 -2.7
168,914 + GFF5946 0.55 -0.3
168,914 + GFF5946 0.55 +0.2
168,914 + GFF5946 0.55 -0.5
168,914 + GFF5946 0.55 -0.1
168,914 + GFF5946 0.55 -0.7
168,914 + GFF5946 0.55 -1.7
168,914 + GFF5946 0.55 -0.5
168,915 - GFF5946 0.55 -0.2
168,915 - GFF5946 0.55 +0.5
168,915 - GFF5946 0.55 -1.2
168,915 - GFF5946 0.55 -0.5
168,915 - GFF5946 0.55 -1.1
168,996 - GFF5946 0.59 -0.5
169,040 + GFF5946 0.61 -0.1
169,040 + GFF5946 0.61 -1.0
169,193 + GFF5946 0.69 -0.6
169,194 - GFF5946 0.69 +0.1
169,194 - GFF5946 0.69 +1.0
169,316 + GFF5946 0.76 +0.7
169,316 + GFF5946 0.76 -1.5
169,317 - GFF5946 0.76 -0.2
169,317 - GFF5946 0.76 -0.4
169,334 + GFF5946 0.77 -1.0
169,334 + GFF5946 0.77 -0.9
169,334 + GFF5946 0.77 -0.5
169,334 + GFF5946 0.77 +0.6
169,334 + GFF5946 0.77 -0.2
169,334 + GFF5946 0.77 -0.5
169,334 + GFF5946 0.77 -1.7
169,334 + GFF5946 0.77 -0.1
169,335 - GFF5946 0.77 -1.0
169,335 - GFF5946 0.77 -2.5
169,335 - GFF5946 0.77 -0.1
169,335 - GFF5946 0.77 -1.2
169,335 - GFF5946 0.77 +1.5
169,335 - GFF5946 0.77 -0.5
169,365 - GFF5946 0.78 -0.3
169,517 + GFF5946 0.86 -1.5
169,517 + GFF5946 0.86 +0.1
169,518 - GFF5946 0.86 -0.2
169,518 - GFF5946 0.86 -0.5
169,518 - GFF5946 0.86 -1.1
169,565 + GFF5946 0.89 -0.3
169,566 - GFF5946 0.89 +0.8
169,591 + GFF5946 0.90 +0.6
169,631 - +0.8
169,753 + -0.0

Or see this region's nucleotide sequence