Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5748 and GFF5749 overlap by 14 nucleotides GFF5749 and GFF5750 overlap by 8 nucleotides GFF5750 and GFF5751 are separated by 290 nucleotides GFF5751 and GFF5752 are separated by 179 nucleotides
GFF5748 - Flagellar assembly protein FliH, at 120,827 to 121,645
GFF5748
GFF5749 - Flagellar motor switch protein FliG, at 121,632 to 122,630
GFF5749
GFF5750 - Flagellar M-ring protein FliF, at 122,623 to 124,359
GFF5750
GFF5751 - Flagellar hook-basal body complex protein FliE, at 124,650 to 124,970
GFF5751
GFF5752 - Flagellin FliC, at 125,150 to 126,805
GFF5752
Position (kb)
122
123
124
125 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 122.404 kb on + strand, within GFF5749 at 122.404 kb on + strand, within GFF5749 at 122.404 kb on + strand, within GFF5749 at 122.404 kb on + strand, within GFF5749 at 122.405 kb on - strand, within GFF5749 at 122.699 kb on + strand at 122.699 kb on + strand at 122.699 kb on + strand at 122.700 kb on - strand at 122.700 kb on - strand at 122.700 kb on - strand at 122.700 kb on - strand at 122.700 kb on - strand at 122.700 kb on - strand at 122.888 kb on + strand, within GFF5750 at 122.888 kb on + strand, within GFF5750 at 122.889 kb on - strand, within GFF5750 at 122.889 kb on - strand, within GFF5750 at 122.897 kb on + strand, within GFF5750 at 123.212 kb on + strand, within GFF5750 at 123.212 kb on + strand, within GFF5750 at 123.213 kb on - strand, within GFF5750 at 123.213 kb on - strand, within GFF5750 at 123.213 kb on - strand, within GFF5750 at 123.503 kb on + strand, within GFF5750 at 123.503 kb on + strand, within GFF5750 at 123.503 kb on + strand, within GFF5750 at 123.503 kb on + strand, within GFF5750 at 123.504 kb on - strand, within GFF5750 at 123.504 kb on - strand, within GFF5750 at 123.504 kb on - strand, within GFF5750 at 123.504 kb on - strand, within GFF5750 at 123.614 kb on + strand, within GFF5750 at 123.615 kb on - strand, within GFF5750 at 123.615 kb on - strand, within GFF5750 at 123.615 kb on - strand, within GFF5750 at 123.638 kb on + strand, within GFF5750 at 123.638 kb on + strand, within GFF5750 at 123.638 kb on + strand, within GFF5750 at 123.639 kb on - strand, within GFF5750 at 123.639 kb on - strand, within GFF5750 at 123.953 kb on + strand, within GFF5750 at 124.118 kb on + strand, within GFF5750 at 124.118 kb on + strand, within GFF5750 at 124.119 kb on - strand, within GFF5750 at 124.182 kb on + strand, within GFF5750 at 124.182 kb on + strand, within GFF5750 at 124.182 kb on + strand, within GFF5750 at 124.183 kb on - strand, within GFF5750 at 124.183 kb on - strand, within GFF5750 at 124.183 kb on - strand, within GFF5750 at 124.184 kb on + strand, within GFF5750 at 124.184 kb on + strand, within GFF5750 at 124.184 kb on + strand, within GFF5750 at 124.184 kb on + strand, within GFF5750 at 124.184 kb on + strand, within GFF5750 at 124.185 kb on - strand, within GFF5750 at 124.185 kb on - strand, within GFF5750 at 124.185 kb on - strand, within GFF5750 at 124.185 kb on - strand, within GFF5750 at 124.185 kb on - strand, within GFF5750 at 124.196 kb on + strand at 124.197 kb on - strand at 124.197 kb on - strand at 124.787 kb on + strand, within GFF5751 at 124.788 kb on - strand, within GFF5751 at 124.812 kb on + strand, within GFF5751 at 124.813 kb on - strand, within GFF5751 at 125.025 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 122,404 + GFF5749 0.77 -0.7 122,404 + GFF5749 0.77 -1.3 122,404 + GFF5749 0.77 -0.2 122,404 + GFF5749 0.77 -1.1 122,405 - GFF5749 0.77 -0.9 122,699 + -1.0 122,699 + -0.7 122,699 + -0.6 122,700 - -0.2 122,700 - -1.1 122,700 - +1.1 122,700 - +2.2 122,700 - -0.6 122,700 - -0.3 122,888 + GFF5750 0.15 -0.3 122,888 + GFF5750 0.15 +1.1 122,889 - GFF5750 0.15 +0.0 122,889 - GFF5750 0.15 -0.1 122,897 + GFF5750 0.16 +0.1 123,212 + GFF5750 0.34 +0.5 123,212 + GFF5750 0.34 +0.2 123,213 - GFF5750 0.34 +0.1 123,213 - GFF5750 0.34 -0.7 123,213 - GFF5750 0.34 +1.7 123,503 + GFF5750 0.51 +1.9 123,503 + GFF5750 0.51 +1.3 123,503 + GFF5750 0.51 +0.1 123,503 + GFF5750 0.51 +0.1 123,504 - GFF5750 0.51 +0.6 123,504 - GFF5750 0.51 +0.6 123,504 - GFF5750 0.51 -1.8 123,504 - GFF5750 0.51 +0.2 123,614 + GFF5750 0.57 +0.5 123,615 - GFF5750 0.57 +1.2 123,615 - GFF5750 0.57 -1.8 123,615 - GFF5750 0.57 -0.6 123,638 + GFF5750 0.58 -0.3 123,638 + GFF5750 0.58 -0.6 123,638 + GFF5750 0.58 +0.7 123,639 - GFF5750 0.58 +0.0 123,639 - GFF5750 0.58 -0.1 123,953 + GFF5750 0.77 -0.6 124,118 + GFF5750 0.86 +2.2 124,118 + GFF5750 0.86 -0.2 124,119 - GFF5750 0.86 -1.3 124,182 + GFF5750 0.90 +0.0 124,182 + GFF5750 0.90 -1.1 124,182 + GFF5750 0.90 +0.1 124,183 - GFF5750 0.90 +0.1 124,183 - GFF5750 0.90 -0.3 124,183 - GFF5750 0.90 +0.0 124,184 + GFF5750 0.90 +0.7 124,184 + GFF5750 0.90 -0.1 124,184 + GFF5750 0.90 -0.2 124,184 + GFF5750 0.90 -1.0 124,184 + GFF5750 0.90 -0.2 124,185 - GFF5750 0.90 +1.7 124,185 - GFF5750 0.90 +0.1 124,185 - GFF5750 0.90 -0.7 124,185 - GFF5750 0.90 -0.9 124,185 - GFF5750 0.90 -0.7 124,196 + -1.1 124,197 - +2.0 124,197 - +0.1 124,787 + GFF5751 0.43 -0.2 124,788 - GFF5751 0.43 -0.0 124,812 + GFF5751 0.50 -1.0 124,813 - GFF5751 0.51 -0.3 125,025 - -0.8
Or see this region's nucleotide sequence