Strain Fitness in Variovorax sp. SCN45 around GFF5750

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5748 and GFF5749 overlap by 14 nucleotidesGFF5749 and GFF5750 overlap by 8 nucleotidesGFF5750 and GFF5751 are separated by 290 nucleotidesGFF5751 and GFF5752 are separated by 179 nucleotides GFF5748 - Flagellar assembly protein FliH, at 120,827 to 121,645 GFF5748 GFF5749 - Flagellar motor switch protein FliG, at 121,632 to 122,630 GFF5749 GFF5750 - Flagellar M-ring protein FliF, at 122,623 to 124,359 GFF5750 GFF5751 - Flagellar hook-basal body complex protein FliE, at 124,650 to 124,970 GFF5751 GFF5752 - Flagellin FliC, at 125,150 to 126,805 GFF5752 Position (kb) 122 123 124 125Strain fitness (log2 ratio) -2 -1 0 1 2at 122.404 kb on + strand, within GFF5749at 122.404 kb on + strand, within GFF5749at 122.404 kb on + strand, within GFF5749at 122.404 kb on + strand, within GFF5749at 122.405 kb on - strand, within GFF5749at 122.699 kb on + strandat 122.699 kb on + strandat 122.699 kb on + strandat 122.700 kb on - strandat 122.700 kb on - strandat 122.700 kb on - strandat 122.700 kb on - strandat 122.700 kb on - strandat 122.700 kb on - strandat 122.888 kb on + strand, within GFF5750at 122.888 kb on + strand, within GFF5750at 122.889 kb on - strand, within GFF5750at 122.889 kb on - strand, within GFF5750at 122.897 kb on + strand, within GFF5750at 123.212 kb on + strand, within GFF5750at 123.212 kb on + strand, within GFF5750at 123.213 kb on - strand, within GFF5750at 123.213 kb on - strand, within GFF5750at 123.213 kb on - strand, within GFF5750at 123.503 kb on + strand, within GFF5750at 123.503 kb on + strand, within GFF5750at 123.503 kb on + strand, within GFF5750at 123.503 kb on + strand, within GFF5750at 123.504 kb on - strand, within GFF5750at 123.504 kb on - strand, within GFF5750at 123.504 kb on - strand, within GFF5750at 123.504 kb on - strand, within GFF5750at 123.614 kb on + strand, within GFF5750at 123.615 kb on - strand, within GFF5750at 123.615 kb on - strand, within GFF5750at 123.615 kb on - strand, within GFF5750at 123.638 kb on + strand, within GFF5750at 123.638 kb on + strand, within GFF5750at 123.638 kb on + strand, within GFF5750at 123.639 kb on - strand, within GFF5750at 123.639 kb on - strand, within GFF5750at 123.953 kb on + strand, within GFF5750at 124.118 kb on + strand, within GFF5750at 124.118 kb on + strand, within GFF5750at 124.119 kb on - strand, within GFF5750at 124.182 kb on + strand, within GFF5750at 124.182 kb on + strand, within GFF5750at 124.182 kb on + strand, within GFF5750at 124.183 kb on - strand, within GFF5750at 124.183 kb on - strand, within GFF5750at 124.183 kb on - strand, within GFF5750at 124.184 kb on + strand, within GFF5750at 124.184 kb on + strand, within GFF5750at 124.184 kb on + strand, within GFF5750at 124.184 kb on + strand, within GFF5750at 124.184 kb on + strand, within GFF5750at 124.185 kb on - strand, within GFF5750at 124.185 kb on - strand, within GFF5750at 124.185 kb on - strand, within GFF5750at 124.185 kb on - strand, within GFF5750at 124.185 kb on - strand, within GFF5750at 124.196 kb on + strandat 124.197 kb on - strandat 124.197 kb on - strandat 124.787 kb on + strand, within GFF5751at 124.788 kb on - strand, within GFF5751at 124.812 kb on + strand, within GFF5751at 124.813 kb on - strand, within GFF5751at 125.025 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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122,404 + GFF5749 0.77 -0.7
122,404 + GFF5749 0.77 -1.3
122,404 + GFF5749 0.77 -0.2
122,404 + GFF5749 0.77 -1.1
122,405 - GFF5749 0.77 -0.9
122,699 + -1.0
122,699 + -0.7
122,699 + -0.6
122,700 - -0.2
122,700 - -1.1
122,700 - +1.1
122,700 - +2.2
122,700 - -0.6
122,700 - -0.3
122,888 + GFF5750 0.15 -0.3
122,888 + GFF5750 0.15 +1.1
122,889 - GFF5750 0.15 +0.0
122,889 - GFF5750 0.15 -0.1
122,897 + GFF5750 0.16 +0.1
123,212 + GFF5750 0.34 +0.5
123,212 + GFF5750 0.34 +0.2
123,213 - GFF5750 0.34 +0.1
123,213 - GFF5750 0.34 -0.7
123,213 - GFF5750 0.34 +1.7
123,503 + GFF5750 0.51 +1.9
123,503 + GFF5750 0.51 +1.3
123,503 + GFF5750 0.51 +0.1
123,503 + GFF5750 0.51 +0.1
123,504 - GFF5750 0.51 +0.6
123,504 - GFF5750 0.51 +0.6
123,504 - GFF5750 0.51 -1.8
123,504 - GFF5750 0.51 +0.2
123,614 + GFF5750 0.57 +0.5
123,615 - GFF5750 0.57 +1.2
123,615 - GFF5750 0.57 -1.8
123,615 - GFF5750 0.57 -0.6
123,638 + GFF5750 0.58 -0.3
123,638 + GFF5750 0.58 -0.6
123,638 + GFF5750 0.58 +0.7
123,639 - GFF5750 0.58 +0.0
123,639 - GFF5750 0.58 -0.1
123,953 + GFF5750 0.77 -0.6
124,118 + GFF5750 0.86 +2.2
124,118 + GFF5750 0.86 -0.2
124,119 - GFF5750 0.86 -1.3
124,182 + GFF5750 0.90 +0.0
124,182 + GFF5750 0.90 -1.1
124,182 + GFF5750 0.90 +0.1
124,183 - GFF5750 0.90 +0.1
124,183 - GFF5750 0.90 -0.3
124,183 - GFF5750 0.90 +0.0
124,184 + GFF5750 0.90 +0.7
124,184 + GFF5750 0.90 -0.1
124,184 + GFF5750 0.90 -0.2
124,184 + GFF5750 0.90 -1.0
124,184 + GFF5750 0.90 -0.2
124,185 - GFF5750 0.90 +1.7
124,185 - GFF5750 0.90 +0.1
124,185 - GFF5750 0.90 -0.7
124,185 - GFF5750 0.90 -0.9
124,185 - GFF5750 0.90 -0.7
124,196 + -1.1
124,197 - +2.0
124,197 - +0.1
124,787 + GFF5751 0.43 -0.2
124,788 - GFF5751 0.43 -0.0
124,812 + GFF5751 0.50 -1.0
124,813 - GFF5751 0.51 -0.3
125,025 - -0.8

Or see this region's nucleotide sequence