Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5673 and GFF5674 are separated by 79 nucleotides GFF5674 and GFF5675 overlap by 1 nucleotides GFF5675 and GFF5676 overlap by 4 nucleotides GFF5676 and GFF5677 are separated by 25 nucleotides
GFF5673 - Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-), at 47,179 to 48,495
GFF5673
GFF5674 - Cytochrome oxidase biogenesis protein Surf1, facilitates heme A insertion, at 48,575 to 49,372
GFF5674
GFF5675 - Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-), at 49,372 to 49,779
GFF5675
GFF5676 - Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-), at 49,776 to 50,384
GFF5676
GFF5677 - Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-), at 50,410 to 52,413
GFF5677
Position (kb)
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 48.425 kb on + strand at 48.425 kb on + strand at 48.681 kb on + strand, within GFF5674 at 48.681 kb on + strand, within GFF5674 at 48.681 kb on + strand, within GFF5674 at 48.681 kb on + strand, within GFF5674 at 48.682 kb on - strand, within GFF5674 at 48.682 kb on - strand, within GFF5674 at 48.682 kb on - strand, within GFF5674 at 48.696 kb on + strand, within GFF5674 at 48.697 kb on - strand, within GFF5674 at 48.697 kb on - strand, within GFF5674 at 48.810 kb on + strand, within GFF5674 at 48.810 kb on + strand, within GFF5674 at 48.810 kb on + strand, within GFF5674 at 48.810 kb on + strand, within GFF5674 at 48.810 kb on + strand, within GFF5674 at 48.810 kb on + strand, within GFF5674 at 48.811 kb on - strand, within GFF5674 at 48.811 kb on - strand, within GFF5674 at 48.811 kb on - strand, within GFF5674 at 48.811 kb on - strand, within GFF5674 at 48.811 kb on - strand, within GFF5674 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.430 kb on + strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.431 kb on - strand, within GFF5675 at 49.538 kb on + strand, within GFF5675 at 49.538 kb on + strand, within GFF5675 at 49.538 kb on + strand, within GFF5675 at 49.538 kb on + strand, within GFF5675 at 49.538 kb on + strand, within GFF5675 at 49.538 kb on + strand, within GFF5675 at 49.538 kb on + strand, within GFF5675 at 49.539 kb on - strand, within GFF5675 at 49.539 kb on - strand, within GFF5675 at 49.539 kb on - strand, within GFF5675 at 49.539 kb on - strand, within GFF5675 at 49.539 kb on - strand, within GFF5675 at 49.539 kb on - strand, within GFF5675 at 49.539 kb on - strand, within GFF5675 at 49.679 kb on + strand, within GFF5675 at 49.679 kb on + strand, within GFF5675 at 49.679 kb on + strand, within GFF5675 at 49.679 kb on + strand, within GFF5675 at 49.679 kb on + strand, within GFF5675 at 49.679 kb on + strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.680 kb on - strand, within GFF5675 at 49.767 kb on + strand at 49.768 kb on - strand at 49.798 kb on + strand at 49.798 kb on + strand at 49.798 kb on + strand at 49.798 kb on + strand at 49.798 kb on + strand at 49.799 kb on - strand at 49.799 kb on - strand at 50.041 kb on + strand, within GFF5676 at 50.041 kb on + strand, within GFF5676 at 50.041 kb on + strand, within GFF5676 at 50.041 kb on + strand, within GFF5676 at 50.042 kb on - strand, within GFF5676 at 50.042 kb on - strand, within GFF5676 at 50.146 kb on + strand, within GFF5676 at 50.146 kb on + strand, within GFF5676 at 50.146 kb on + strand, within GFF5676 at 50.146 kb on + strand, within GFF5676 at 50.147 kb on - strand, within GFF5676 at 50.147 kb on - strand, within GFF5676 at 50.147 kb on - strand, within GFF5676 at 50.147 kb on - strand, within GFF5676 at 50.233 kb on + strand, within GFF5676 at 50.233 kb on + strand, within GFF5676 at 50.233 kb on + strand, within GFF5676 at 50.234 kb on - strand, within GFF5676 at 50.254 kb on + strand, within GFF5676 at 50.254 kb on + strand, within GFF5676 at 50.254 kb on + strand, within GFF5676 at 50.254 kb on + strand, within GFF5676 at 50.254 kb on + strand, within GFF5676 at 50.255 kb on - strand, within GFF5676 at 50.255 kb on - strand, within GFF5676 at 50.255 kb on - strand, within GFF5676 at 50.299 kb on + strand, within GFF5676 at 50.299 kb on + strand, within GFF5676 at 50.299 kb on + strand, within GFF5676 at 50.300 kb on - strand, within GFF5676 at 50.408 kb on + strand at 50.408 kb on + strand at 50.408 kb on + strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.409 kb on - strand at 50.411 kb on + strand at 50.411 kb on + strand at 50.411 kb on + strand at 50.411 kb on + strand at 50.411 kb on + strand at 50.411 kb on + strand at 50.411 kb on + strand at 50.412 kb on - strand at 50.412 kb on - strand at 50.412 kb on - strand at 50.412 kb on - strand at 50.412 kb on - strand at 50.412 kb on - strand at 50.412 kb on - strand at 50.480 kb on + strand at 50.481 kb on - strand at 50.481 kb on - strand at 50.481 kb on - strand at 50.481 kb on - strand at 50.492 kb on + strand at 50.492 kb on + strand at 50.492 kb on + strand at 50.492 kb on + strand at 50.492 kb on + strand at 50.492 kb on + strand at 50.493 kb on - strand at 50.493 kb on - strand at 50.493 kb on - strand at 50.493 kb on - strand at 50.493 kb on - strand at 50.502 kb on + strand at 50.502 kb on + strand at 50.502 kb on + strand at 50.502 kb on + strand at 50.502 kb on + strand at 50.502 kb on + strand at 50.502 kb on + strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.503 kb on - strand at 50.735 kb on + strand, within GFF5677 at 50.735 kb on + strand, within GFF5677 at 50.735 kb on + strand, within GFF5677 at 50.735 kb on + strand, within GFF5677 at 50.736 kb on - strand, within GFF5677 at 50.736 kb on - strand, within GFF5677 at 50.736 kb on - strand, within GFF5677 at 50.736 kb on - strand, within GFF5677
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 48,425 + -0.3 48,425 + -0.3 48,681 + GFF5674 0.13 -0.3 48,681 + GFF5674 0.13 -1.1 48,681 + GFF5674 0.13 +0.4 48,681 + GFF5674 0.13 +0.5 48,682 - GFF5674 0.13 -0.2 48,682 - GFF5674 0.13 +0.2 48,682 - GFF5674 0.13 -0.5 48,696 + GFF5674 0.15 -0.8 48,697 - GFF5674 0.15 +0.1 48,697 - GFF5674 0.15 +0.4 48,810 + GFF5674 0.29 +0.3 48,810 + GFF5674 0.29 +0.0 48,810 + GFF5674 0.29 -0.1 48,810 + GFF5674 0.29 +1.4 48,810 + GFF5674 0.29 -0.2 48,810 + GFF5674 0.29 +0.3 48,811 - GFF5674 0.30 -1.6 48,811 - GFF5674 0.30 -0.7 48,811 - GFF5674 0.30 +3.3 48,811 - GFF5674 0.30 -0.9 48,811 - GFF5674 0.30 +1.0 49,430 + GFF5675 0.14 +0.1 49,430 + GFF5675 0.14 -0.1 49,430 + GFF5675 0.14 -0.1 49,430 + GFF5675 0.14 +0.1 49,430 + GFF5675 0.14 -1.2 49,430 + GFF5675 0.14 -0.5 49,430 + GFF5675 0.14 -0.4 49,430 + GFF5675 0.14 -0.2 49,430 + GFF5675 0.14 -0.1 49,430 + GFF5675 0.14 +0.3 49,431 - GFF5675 0.14 +1.5 49,431 - GFF5675 0.14 +0.0 49,431 - GFF5675 0.14 +0.0 49,431 - GFF5675 0.14 -0.1 49,431 - GFF5675 0.14 -2.3 49,431 - GFF5675 0.14 -1.0 49,431 - GFF5675 0.14 +1.9 49,431 - GFF5675 0.14 +0.0 49,538 + GFF5675 0.41 -0.8 49,538 + GFF5675 0.41 -0.6 49,538 + GFF5675 0.41 +0.7 49,538 + GFF5675 0.41 +0.1 49,538 + GFF5675 0.41 -0.3 49,538 + GFF5675 0.41 +0.0 49,538 + GFF5675 0.41 -3.1 49,539 - GFF5675 0.41 +0.5 49,539 - GFF5675 0.41 -0.7 49,539 - GFF5675 0.41 +0.5 49,539 - GFF5675 0.41 -0.9 49,539 - GFF5675 0.41 -0.9 49,539 - GFF5675 0.41 -0.6 49,539 - GFF5675 0.41 +0.1 49,679 + GFF5675 0.75 +0.3 49,679 + GFF5675 0.75 -2.0 49,679 + GFF5675 0.75 -1.0 49,679 + GFF5675 0.75 +2.5 49,679 + GFF5675 0.75 -0.6 49,679 + GFF5675 0.75 -0.2 49,680 - GFF5675 0.75 -1.9 49,680 - GFF5675 0.75 -1.0 49,680 - GFF5675 0.75 +0.9 49,680 - GFF5675 0.75 +1.7 49,680 - GFF5675 0.75 -1.8 49,680 - GFF5675 0.75 +0.6 49,680 - GFF5675 0.75 -1.1 49,680 - GFF5675 0.75 -0.8 49,767 + +0.3 49,768 - +0.5 49,798 + -2.2 49,798 + -0.4 49,798 + -0.3 49,798 + -1.0 49,798 + +0.5 49,799 - -0.1 49,799 - +0.0 50,041 + GFF5676 0.44 -0.6 50,041 + GFF5676 0.44 +0.9 50,041 + GFF5676 0.44 +0.9 50,041 + GFF5676 0.44 -0.7 50,042 - GFF5676 0.44 -0.9 50,042 - GFF5676 0.44 +0.0 50,146 + GFF5676 0.61 +0.7 50,146 + GFF5676 0.61 +0.1 50,146 + GFF5676 0.61 +0.2 50,146 + GFF5676 0.61 -2.4 50,147 - GFF5676 0.61 +0.1 50,147 - GFF5676 0.61 -0.6 50,147 - GFF5676 0.61 +0.4 50,147 - GFF5676 0.61 -0.2 50,233 + GFF5676 0.75 -0.1 50,233 + GFF5676 0.75 +0.4 50,233 + GFF5676 0.75 -0.3 50,234 - GFF5676 0.75 +0.5 50,254 + GFF5676 0.78 +0.0 50,254 + GFF5676 0.78 +0.7 50,254 + GFF5676 0.78 -1.3 50,254 + GFF5676 0.78 +0.5 50,254 + GFF5676 0.78 -1.0 50,255 - GFF5676 0.79 -0.9 50,255 - GFF5676 0.79 -1.3 50,255 - GFF5676 0.79 +2.4 50,299 + GFF5676 0.86 +2.0 50,299 + GFF5676 0.86 +0.8 50,299 + GFF5676 0.86 +0.6 50,300 - GFF5676 0.86 +0.1 50,408 + -0.2 50,408 + -0.4 50,408 + +1.7 50,409 - +0.1 50,409 - +1.0 50,409 - +0.7 50,409 - +0.3 50,409 - +1.7 50,409 - +0.4 50,409 - +0.1 50,409 - +0.7 50,409 - -1.1 50,411 + -1.2 50,411 + -0.5 50,411 + +0.3 50,411 + +0.5 50,411 + -0.3 50,411 + -2.0 50,411 + -1.3 50,412 - -0.1 50,412 - +0.6 50,412 - -0.9 50,412 - +0.1 50,412 - -0.0 50,412 - -0.4 50,412 - +0.7 50,480 + +0.8 50,481 - -0.5 50,481 - -0.5 50,481 - +0.7 50,481 - -0.0 50,492 + +1.0 50,492 + -1.1 50,492 + -0.5 50,492 + -0.4 50,492 + -0.3 50,492 + -1.0 50,493 - -0.4 50,493 - +0.7 50,493 - +1.4 50,493 - -0.2 50,493 - +0.1 50,502 + -0.2 50,502 + +0.5 50,502 + +0.2 50,502 + +0.6 50,502 + +0.5 50,502 + +1.8 50,502 + +1.0 50,503 - -1.6 50,503 - +0.9 50,503 - -1.6 50,503 - -0.4 50,503 - +1.2 50,503 - -0.3 50,503 - +0.2 50,503 - -1.3 50,503 - +1.7 50,735 + GFF5677 0.16 +1.2 50,735 + GFF5677 0.16 -1.3 50,735 + GFF5677 0.16 +0.7 50,735 + GFF5677 0.16 -0.6 50,736 - GFF5677 0.16 +0.7 50,736 - GFF5677 0.16 +1.2 50,736 - GFF5677 0.16 +0.0 50,736 - GFF5677 0.16 +0.3
Or see this region's nucleotide sequence