Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5644 and GFF5645 are separated by 24 nucleotides GFF5645 and GFF5646 overlap by 4 nucleotides GFF5646 and GFF5647 overlap by 4 nucleotides
GFF5644 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 19,318 to 20,367
GFF5644
GFF5645 - ABC transporter, permease protein (cluster 12, methionine/phosphonates) / ABC transporter, permease protein (cluster 12, methionine/phosphonates), at 20,392 to 21,201
GFF5645
GFF5646 - ABC transporter, permease protein (cluster 12, methionine/phosphonates) / ABC transporter, permease protein (cluster 12, methionine/phosphonates), at 21,198 to 22,037
GFF5646
GFF5647 - ABC transporter, ATP-binding protein (cluster 12, methionine/phosphonates), at 22,034 to 22,846
GFF5647
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2 at 19.436 kb on + strand, within GFF5644 at 19.436 kb on + strand, within GFF5644 at 19.436 kb on + strand, within GFF5644 at 19.448 kb on + strand, within GFF5644 at 19.448 kb on + strand, within GFF5644 at 19.448 kb on + strand, within GFF5644 at 19.448 kb on + strand, within GFF5644 at 19.449 kb on - strand, within GFF5644 at 19.449 kb on - strand, within GFF5644 at 19.449 kb on - strand, within GFF5644 at 19.449 kb on - strand, within GFF5644 at 19.449 kb on - strand, within GFF5644 at 19.592 kb on + strand, within GFF5644 at 19.592 kb on + strand, within GFF5644 at 19.593 kb on - strand, within GFF5644 at 19.593 kb on - strand, within GFF5644 at 19.593 kb on - strand, within GFF5644 at 19.599 kb on - strand, within GFF5644 at 19.599 kb on - strand, within GFF5644 at 19.599 kb on - strand, within GFF5644 at 19.599 kb on - strand, within GFF5644 at 19.599 kb on - strand, within GFF5644 at 19.613 kb on + strand, within GFF5644 at 19.613 kb on + strand, within GFF5644 at 19.730 kb on + strand, within GFF5644 at 19.730 kb on + strand, within GFF5644 at 19.730 kb on + strand, within GFF5644 at 19.730 kb on + strand, within GFF5644 at 19.730 kb on + strand, within GFF5644 at 19.731 kb on - strand, within GFF5644 at 19.731 kb on - strand, within GFF5644 at 19.731 kb on - strand, within GFF5644 at 19.731 kb on - strand, within GFF5644 at 20.003 kb on + strand, within GFF5644 at 20.004 kb on - strand, within GFF5644 at 20.090 kb on + strand, within GFF5644 at 20.090 kb on + strand, within GFF5644 at 20.090 kb on + strand, within GFF5644 at 20.090 kb on + strand, within GFF5644 at 20.090 kb on + strand, within GFF5644 at 20.090 kb on + strand, within GFF5644 at 20.090 kb on + strand, within GFF5644 at 20.091 kb on - strand, within GFF5644 at 20.091 kb on - strand, within GFF5644 at 20.091 kb on - strand, within GFF5644 at 20.091 kb on - strand, within GFF5644 at 20.091 kb on - strand, within GFF5644 at 20.210 kb on + strand, within GFF5644 at 20.210 kb on + strand, within GFF5644 at 20.211 kb on - strand, within GFF5644 at 20.211 kb on - strand, within GFF5644 at 20.274 kb on + strand at 20.274 kb on + strand at 20.274 kb on + strand at 20.275 kb on - strand at 20.275 kb on - strand at 20.399 kb on + strand at 20.399 kb on + strand at 20.399 kb on + strand at 20.399 kb on + strand at 20.400 kb on - strand at 20.400 kb on - strand at 20.400 kb on - strand at 20.519 kb on + strand, within GFF5645 at 20.519 kb on + strand, within GFF5645 at 20.520 kb on - strand, within GFF5645 at 20.520 kb on - strand, within GFF5645 at 20.594 kb on + strand, within GFF5645 at 20.642 kb on + strand, within GFF5645 at 20.642 kb on + strand, within GFF5645 at 20.643 kb on - strand, within GFF5645 at 21.098 kb on + strand, within GFF5645 at 21.388 kb on + strand, within GFF5646 at 21.388 kb on + strand, within GFF5646 at 21.388 kb on + strand, within GFF5646 at 21.388 kb on + strand, within GFF5646 at 21.389 kb on - strand, within GFF5646 at 21.389 kb on - strand, within GFF5646 at 21.397 kb on + strand, within GFF5646 at 21.397 kb on + strand, within GFF5646 at 21.520 kb on + strand, within GFF5646 at 21.521 kb on - strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.120 kb on - strand, within GFF5647
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 19,436 + GFF5644 0.11 +0.4 19,436 + GFF5644 0.11 +0.0 19,436 + GFF5644 0.11 -0.5 19,448 + GFF5644 0.12 -0.3 19,448 + GFF5644 0.12 -0.5 19,448 + GFF5644 0.12 +0.1 19,448 + GFF5644 0.12 -0.8 19,449 - GFF5644 0.12 -1.5 19,449 - GFF5644 0.12 +0.0 19,449 - GFF5644 0.12 -0.9 19,449 - GFF5644 0.12 -0.9 19,449 - GFF5644 0.12 +0.4 19,592 + GFF5644 0.26 -0.6 19,592 + GFF5644 0.26 -1.5 19,593 - GFF5644 0.26 -1.1 19,593 - GFF5644 0.26 -0.8 19,593 - GFF5644 0.26 +0.1 19,599 - GFF5644 0.27 -0.5 19,599 - GFF5644 0.27 -0.3 19,599 - GFF5644 0.27 +0.1 19,599 - GFF5644 0.27 +0.0 19,599 - GFF5644 0.27 -0.1 19,613 + GFF5644 0.28 +0.2 19,613 + GFF5644 0.28 +1.4 19,730 + GFF5644 0.39 -0.0 19,730 + GFF5644 0.39 -1.5 19,730 + GFF5644 0.39 +0.2 19,730 + GFF5644 0.39 +0.8 19,730 + GFF5644 0.39 -0.3 19,731 - GFF5644 0.39 -0.8 19,731 - GFF5644 0.39 -0.8 19,731 - GFF5644 0.39 +0.2 19,731 - GFF5644 0.39 +1.5 20,003 + GFF5644 0.65 +0.7 20,004 - GFF5644 0.65 -1.0 20,090 + GFF5644 0.74 +0.6 20,090 + GFF5644 0.74 -0.7 20,090 + GFF5644 0.74 -0.6 20,090 + GFF5644 0.74 +1.7 20,090 + GFF5644 0.74 -0.1 20,090 + GFF5644 0.74 +0.1 20,090 + GFF5644 0.74 +0.1 20,091 - GFF5644 0.74 -0.1 20,091 - GFF5644 0.74 +0.7 20,091 - GFF5644 0.74 +0.8 20,091 - GFF5644 0.74 -0.4 20,091 - GFF5644 0.74 +0.7 20,210 + GFF5644 0.85 -0.0 20,210 + GFF5644 0.85 -2.2 20,211 - GFF5644 0.85 -1.9 20,211 - GFF5644 0.85 -0.7 20,274 + -0.0 20,274 + +1.0 20,274 + -1.0 20,275 - -0.6 20,275 - -0.2 20,399 + -0.8 20,399 + -0.9 20,399 + -0.6 20,399 + -0.1 20,400 - +1.8 20,400 - -0.1 20,400 - -0.6 20,519 + GFF5645 0.16 -1.8 20,519 + GFF5645 0.16 -0.2 20,520 - GFF5645 0.16 +0.1 20,520 - GFF5645 0.16 -1.3 20,594 + GFF5645 0.25 -0.2 20,642 + GFF5645 0.31 -0.6 20,642 + GFF5645 0.31 -1.7 20,643 - GFF5645 0.31 -0.3 21,098 + GFF5645 0.87 -1.1 21,388 + GFF5646 0.23 -0.5 21,388 + GFF5646 0.23 +0.1 21,388 + GFF5646 0.23 -0.1 21,388 + GFF5646 0.23 -0.6 21,389 - GFF5646 0.23 -0.1 21,389 - GFF5646 0.23 -0.9 21,397 + GFF5646 0.24 -1.0 21,397 + GFF5646 0.24 -1.0 21,520 + GFF5646 0.38 +1.7 21,521 - GFF5646 0.38 -0.5 21,544 + GFF5646 0.41 +0.2 21,544 + GFF5646 0.41 +1.7 21,544 + GFF5646 0.41 -1.0 21,544 + GFF5646 0.41 -0.6 21,544 + GFF5646 0.41 +0.2 21,545 - GFF5646 0.41 +0.3 21,545 - GFF5646 0.41 +0.0 21,545 - GFF5646 0.41 +0.9 21,545 - GFF5646 0.41 -0.6 21,545 - GFF5646 0.41 -1.1 21,545 - GFF5646 0.41 +0.7 21,545 - GFF5646 0.41 +0.4 21,545 - GFF5646 0.41 -0.6 21,545 - GFF5646 0.41 -1.1 21,545 - GFF5646 0.41 -0.7 22,119 + GFF5647 0.10 +2.0 22,119 + GFF5647 0.10 +0.9 22,119 + GFF5647 0.10 -0.2 22,120 - GFF5647 0.11 -0.0
Or see this region's nucleotide sequence