Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5606 and GFF5607 are separated by 4 nucleotides GFF5607 and GFF5608 are separated by 136 nucleotides
GFF5606 - Xylose-responsive transcription regulator, ROK family, at 224,876 to 226,039
GFF5606
GFF5607 - Sigma-54 activated regulatory protein, at 226,044 to 227,324
GFF5607
GFF5608 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.2.8), at 227,461 to 229,575
GFF5608
Position (kb)
226
227
228 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 225.194 kb on + strand, within GFF5606 at 225.492 kb on - strand, within GFF5606 at 225.492 kb on - strand, within GFF5606 at 225.645 kb on + strand, within GFF5606 at 225.645 kb on + strand, within GFF5606 at 225.645 kb on + strand, within GFF5606 at 225.645 kb on + strand, within GFF5606 at 225.972 kb on - strand at 225.972 kb on - strand at 225.972 kb on - strand at 226.093 kb on + strand at 226.093 kb on + strand at 226.226 kb on + strand, within GFF5607 at 226.227 kb on - strand, within GFF5607 at 226.620 kb on + strand, within GFF5607 at 226.621 kb on - strand, within GFF5607 at 226.621 kb on - strand, within GFF5607 at 226.675 kb on + strand, within GFF5607 at 226.675 kb on + strand, within GFF5607 at 226.675 kb on + strand, within GFF5607 at 226.676 kb on - strand, within GFF5607 at 226.810 kb on + strand, within GFF5607 at 226.810 kb on + strand, within GFF5607 at 226.810 kb on + strand, within GFF5607 at 226.810 kb on + strand, within GFF5607 at 226.810 kb on + strand, within GFF5607 at 226.810 kb on + strand, within GFF5607 at 226.811 kb on - strand, within GFF5607 at 226.811 kb on - strand, within GFF5607 at 226.811 kb on - strand, within GFF5607 at 226.811 kb on - strand, within GFF5607 at 226.811 kb on - strand, within GFF5607 at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.507 kb on + strand at 227.508 kb on - strand at 227.508 kb on - strand at 227.508 kb on - strand at 227.508 kb on - strand at 227.508 kb on - strand at 227.508 kb on - strand at 227.508 kb on - strand at 227.508 kb on - strand at 227.594 kb on + strand at 227.594 kb on + strand at 227.595 kb on - strand at 227.595 kb on - strand at 227.595 kb on - strand at 227.725 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.785 kb on + strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.786 kb on - strand, within GFF5608 at 227.938 kb on + strand, within GFF5608 at 227.938 kb on + strand, within GFF5608 at 227.938 kb on + strand, within GFF5608 at 227.938 kb on + strand, within GFF5608 at 227.939 kb on - strand, within GFF5608 at 227.939 kb on - strand, within GFF5608 at 227.939 kb on - strand, within GFF5608 at 227.939 kb on - strand, within GFF5608 at 227.939 kb on - strand, within GFF5608 at 227.939 kb on - strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.034 kb on + strand, within GFF5608 at 228.035 kb on - strand, within GFF5608 at 228.035 kb on - strand, within GFF5608 at 228.035 kb on - strand, within GFF5608 at 228.035 kb on - strand, within GFF5608 at 228.035 kb on - strand, within GFF5608 at 228.035 kb on - strand, within GFF5608 at 228.100 kb on + strand, within GFF5608 at 228.247 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.316 kb on + strand, within GFF5608 at 228.317 kb on + strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608 at 228.317 kb on - strand, within GFF5608
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 225,194 + GFF5606 0.27 +0.3 225,492 - GFF5606 0.53 +0.8 225,492 - GFF5606 0.53 +0.2 225,645 + GFF5606 0.66 +1.2 225,645 + GFF5606 0.66 +0.3 225,645 + GFF5606 0.66 +0.0 225,645 + GFF5606 0.66 +1.3 225,972 - +0.5 225,972 - +0.5 225,972 - +0.9 226,093 + -0.4 226,093 + -0.8 226,226 + GFF5607 0.14 -1.7 226,227 - GFF5607 0.14 +0.1 226,620 + GFF5607 0.45 -1.1 226,621 - GFF5607 0.45 -1.8 226,621 - GFF5607 0.45 -0.9 226,675 + GFF5607 0.49 -0.9 226,675 + GFF5607 0.49 +0.3 226,675 + GFF5607 0.49 +0.7 226,676 - GFF5607 0.49 +0.0 226,810 + GFF5607 0.60 -0.3 226,810 + GFF5607 0.60 -0.6 226,810 + GFF5607 0.60 -0.3 226,810 + GFF5607 0.60 -1.4 226,810 + GFF5607 0.60 -1.7 226,810 + GFF5607 0.60 -0.4 226,811 - GFF5607 0.60 -0.6 226,811 - GFF5607 0.60 +0.4 226,811 - GFF5607 0.60 -0.5 226,811 - GFF5607 0.60 -0.5 226,811 - GFF5607 0.60 -0.5 227,507 + -0.3 227,507 + -0.6 227,507 + -0.5 227,507 + +0.5 227,507 + +0.7 227,507 + -1.1 227,507 + -0.3 227,507 + +0.1 227,507 + -0.6 227,507 + -0.5 227,507 + -0.7 227,508 - -0.1 227,508 - -1.7 227,508 - -0.1 227,508 - -1.1 227,508 - -1.5 227,508 - -1.2 227,508 - -0.3 227,508 - -0.3 227,594 + -0.9 227,594 + +0.8 227,595 - -0.0 227,595 - -0.5 227,595 - -0.1 227,725 + GFF5608 0.12 -2.4 227,785 + GFF5608 0.15 +0.4 227,785 + GFF5608 0.15 -0.5 227,785 + GFF5608 0.15 -0.0 227,785 + GFF5608 0.15 -0.1 227,785 + GFF5608 0.15 +0.6 227,785 + GFF5608 0.15 -0.1 227,785 + GFF5608 0.15 -1.5 227,785 + GFF5608 0.15 -0.4 227,785 + GFF5608 0.15 -1.5 227,785 + GFF5608 0.15 +0.1 227,785 + GFF5608 0.15 +1.0 227,785 + GFF5608 0.15 -1.3 227,785 + GFF5608 0.15 -0.7 227,785 + GFF5608 0.15 -0.4 227,785 + GFF5608 0.15 -0.8 227,785 + GFF5608 0.15 -0.2 227,785 + GFF5608 0.15 +0.9 227,785 + GFF5608 0.15 +0.6 227,785 + GFF5608 0.15 +0.8 227,786 - GFF5608 0.15 -1.3 227,786 - GFF5608 0.15 +0.8 227,786 - GFF5608 0.15 +0.4 227,786 - GFF5608 0.15 -1.2 227,786 - GFF5608 0.15 -0.3 227,786 - GFF5608 0.15 -0.5 227,786 - GFF5608 0.15 -1.3 227,786 - GFF5608 0.15 +0.9 227,786 - GFF5608 0.15 +0.7 227,786 - GFF5608 0.15 -0.2 227,786 - GFF5608 0.15 -0.3 227,786 - GFF5608 0.15 +0.0 227,786 - GFF5608 0.15 +1.2 227,786 - GFF5608 0.15 -0.7 227,786 - GFF5608 0.15 -1.0 227,786 - GFF5608 0.15 -0.3 227,938 + GFF5608 0.23 +0.8 227,938 + GFF5608 0.23 +1.0 227,938 + GFF5608 0.23 -0.2 227,938 + GFF5608 0.23 +0.0 227,939 - GFF5608 0.23 +0.7 227,939 - GFF5608 0.23 +0.2 227,939 - GFF5608 0.23 -0.2 227,939 - GFF5608 0.23 -0.3 227,939 - GFF5608 0.23 +0.6 227,939 - GFF5608 0.23 +0.4 228,034 + GFF5608 0.27 -0.2 228,034 + GFF5608 0.27 +0.6 228,034 + GFF5608 0.27 +0.1 228,034 + GFF5608 0.27 -0.2 228,034 + GFF5608 0.27 +0.7 228,034 + GFF5608 0.27 +0.7 228,034 + GFF5608 0.27 -0.7 228,034 + GFF5608 0.27 -1.1 228,035 - GFF5608 0.27 -0.4 228,035 - GFF5608 0.27 -0.8 228,035 - GFF5608 0.27 -0.0 228,035 - GFF5608 0.27 +0.5 228,035 - GFF5608 0.27 +0.6 228,035 - GFF5608 0.27 -1.4 228,100 + GFF5608 0.30 -1.9 228,247 + GFF5608 0.37 +0.9 228,316 + GFF5608 0.40 +0.7 228,316 + GFF5608 0.40 -0.1 228,316 + GFF5608 0.40 -0.6 228,316 + GFF5608 0.40 +0.1 228,316 + GFF5608 0.40 +1.8 228,316 + GFF5608 0.40 -0.2 228,316 + GFF5608 0.40 +0.4 228,316 + GFF5608 0.40 -0.7 228,316 + GFF5608 0.40 -1.4 228,316 + GFF5608 0.40 -0.9 228,317 + GFF5608 0.40 +0.5 228,317 - GFF5608 0.40 +0.1 228,317 - GFF5608 0.40 +0.5 228,317 - GFF5608 0.40 +1.6 228,317 - GFF5608 0.40 +0.7 228,317 - GFF5608 0.40 -1.3 228,317 - GFF5608 0.40 +0.1 228,317 - GFF5608 0.40 -0.5 228,317 - GFF5608 0.40 -0.7 228,317 - GFF5608 0.40 +1.0 228,317 - GFF5608 0.40 +1.9
Or see this region's nucleotide sequence