Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5528 and GFF5529 overlap by 4 nucleotides GFF5529 and GFF5530 are separated by 18 nucleotides GFF5530 and GFF5531 are separated by 107 nucleotides GFF5531 and GFF5532 are separated by 69 nucleotides
GFF5528 - Conjugative transfer protein TrbL, at 158,320 to 158,799
GFF5528
GFF5529 - Putative outer membrane protein, at 158,796 to 159,599
GFF5529
GFF5530 - FIG00931903: hypothetical protein, at 159,618 to 160,655
GFF5530
GFF5531 - hypothetical protein, at 160,763 to 161,158
GFF5531
GFF5532 - Putative oxidoreductase, at 161,228 to 162,010
GFF5532
Position (kb) 159
160
161 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 158.779 kb on + strand at 158.779 kb on + strand at 158.779 kb on + strand at 158.779 kb on + strand at 158.779 kb on + strand at 158.780 kb on - strand at 158.780 kb on - strand at 158.780 kb on - strand at 158.780 kb on - strand at 158.780 kb on - strand at 159.012 kb on + strand, within GFF5529 at 159.012 kb on + strand, within GFF5529 at 159.012 kb on + strand, within GFF5529 at 159.012 kb on + strand, within GFF5529 at 159.012 kb on + strand, within GFF5529 at 159.012 kb on + strand, within GFF5529 at 159.012 kb on + strand, within GFF5529 at 159.012 kb on + strand, within GFF5529 at 159.013 kb on - strand, within GFF5529 at 159.013 kb on - strand, within GFF5529 at 159.013 kb on - strand, within GFF5529 at 159.013 kb on - strand, within GFF5529 at 159.013 kb on - strand, within GFF5529 at 159.013 kb on - strand, within GFF5529 at 159.024 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.045 kb on + strand, within GFF5529 at 159.046 kb on - strand, within GFF5529 at 159.046 kb on - strand, within GFF5529 at 159.117 kb on + strand, within GFF5529 at 159.117 kb on + strand, within GFF5529 at 159.117 kb on + strand, within GFF5529 at 159.118 kb on - strand, within GFF5529 at 159.163 kb on - strand, within GFF5529 at 159.264 kb on + strand, within GFF5529 at 159.264 kb on + strand, within GFF5529 at 159.264 kb on + strand, within GFF5529 at 159.264 kb on + strand, within GFF5529 at 159.265 kb on - strand, within GFF5529 at 159.265 kb on - strand, within GFF5529 at 159.265 kb on - strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.279 kb on + strand, within GFF5529 at 159.280 kb on - strand, within GFF5529 at 159.280 kb on - strand, within GFF5529 at 159.297 kb on + strand, within GFF5529 at 159.297 kb on + strand, within GFF5529 at 159.297 kb on + strand, within GFF5529 at 159.297 kb on + strand, within GFF5529 at 159.298 kb on - strand, within GFF5529 at 159.298 kb on - strand, within GFF5529 at 159.298 kb on - strand, within GFF5529 at 159.298 kb on - strand, within GFF5529 at 159.298 kb on - strand, within GFF5529 at 159.298 kb on - strand, within GFF5529 at 159.298 kb on - strand, within GFF5529 at 159.402 kb on + strand, within GFF5529 at 159.402 kb on + strand, within GFF5529 at 159.402 kb on + strand, within GFF5529 at 159.424 kb on + strand, within GFF5529 at 159.424 kb on + strand, within GFF5529 at 159.425 kb on - strand, within GFF5529 at 159.856 kb on + strand, within GFF5530 at 159.856 kb on + strand, within GFF5530 at 159.856 kb on + strand, within GFF5530 at 159.856 kb on + strand, within GFF5530 at 159.856 kb on + strand, within GFF5530 at 159.856 kb on + strand, within GFF5530 at 159.857 kb on - strand, within GFF5530 at 159.857 kb on - strand, within GFF5530 at 159.857 kb on - strand, within GFF5530 at 159.857 kb on - strand, within GFF5530 at 159.857 kb on - strand, within GFF5530 at 160.137 kb on + strand, within GFF5530 at 160.239 kb on + strand, within GFF5530 at 160.239 kb on + strand, within GFF5530 at 160.239 kb on + strand, within GFF5530 at 160.239 kb on + strand, within GFF5530 at 160.239 kb on + strand, within GFF5530 at 160.240 kb on - strand, within GFF5530 at 160.240 kb on - strand, within GFF5530 at 160.240 kb on - strand, within GFF5530 at 160.240 kb on - strand, within GFF5530 at 160.240 kb on - strand, within GFF5530 at 160.305 kb on + strand, within GFF5530 at 160.305 kb on + strand, within GFF5530 at 160.305 kb on + strand, within GFF5530 at 160.306 kb on - strand, within GFF5530 at 160.306 kb on - strand, within GFF5530 at 160.306 kb on - strand, within GFF5530 at 160.317 kb on + strand, within GFF5530 at 160.317 kb on + strand, within GFF5530 at 160.318 kb on - strand, within GFF5530 at 160.318 kb on - strand, within GFF5530 at 160.326 kb on + strand, within GFF5530 at 160.326 kb on + strand, within GFF5530 at 160.326 kb on + strand, within GFF5530 at 160.326 kb on + strand, within GFF5530 at 160.326 kb on + strand, within GFF5530 at 160.327 kb on - strand, within GFF5530 at 160.327 kb on - strand, within GFF5530 at 160.335 kb on + strand, within GFF5530 at 160.335 kb on + strand, within GFF5530 at 160.335 kb on + strand, within GFF5530 at 160.335 kb on + strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.336 kb on - strand, within GFF5530 at 160.375 kb on - strand, within GFF5530 at 160.375 kb on - strand, within GFF5530 at 160.398 kb on + strand, within GFF5530 at 160.398 kb on + strand, within GFF5530 at 160.461 kb on + strand, within GFF5530 at 160.461 kb on + strand, within GFF5530 at 160.461 kb on + strand, within GFF5530 at 160.461 kb on + strand, within GFF5530 at 160.462 kb on - strand, within GFF5530 at 160.462 kb on - strand, within GFF5530 at 160.462 kb on - strand, within GFF5530 at 160.653 kb on + strand at 160.653 kb on + strand at 160.653 kb on + strand at 160.653 kb on + strand at 160.654 kb on - strand at 160.700 kb on + strand at 160.700 kb on + strand at 160.701 kb on - strand at 160.701 kb on - strand at 160.701 kb on - strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.725 kb on + strand at 160.726 kb on - strand at 160.726 kb on - strand at 160.726 kb on - strand at 160.726 kb on - strand at 160.726 kb on - strand at 160.726 kb on - strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.757 kb on + strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 160.758 kb on - strand at 161.048 kb on + strand, within GFF5531 at 161.049 kb on - strand, within GFF5531 at 161.049 kb on - strand, within GFF5531 at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.253 kb on + strand at 161.254 kb on - strand at 161.254 kb on - strand at 161.254 kb on - strand at 161.254 kb on - strand at 161.254 kb on - strand at 161.254 kb on - strand at 161.254 kb on - strand at 161.431 kb on - strand, within GFF5532 at 161.511 kb on + strand, within GFF5532 at 161.511 kb on + strand, within GFF5532 at 161.511 kb on + strand, within GFF5532 at 161.512 kb on - strand, within GFF5532 at 161.512 kb on - strand, within GFF5532 at 161.512 kb on - strand, within GFF5532 at 161.512 kb on - strand, within GFF5532 at 161.512 kb on - strand, within GFF5532 at 161.520 kb on + strand, within GFF5532
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 158,779 + +0.0 158,779 + +0.8 158,779 + -0.2 158,779 + +0.5 158,779 + +0.2 158,780 - -0.1 158,780 - -0.3 158,780 - -0.9 158,780 - -1.1 158,780 - +0.2 159,012 + GFF5529 0.27 +0.9 159,012 + GFF5529 0.27 -1.0 159,012 + GFF5529 0.27 -1.5 159,012 + GFF5529 0.27 +0.5 159,012 + GFF5529 0.27 -0.3 159,012 + GFF5529 0.27 -0.2 159,012 + GFF5529 0.27 -0.3 159,012 + GFF5529 0.27 +1.2 159,013 - GFF5529 0.27 +0.1 159,013 - GFF5529 0.27 +0.2 159,013 - GFF5529 0.27 -0.5 159,013 - GFF5529 0.27 -0.8 159,013 - GFF5529 0.27 +0.9 159,013 - GFF5529 0.27 +0.0 159,024 + GFF5529 0.28 +0.5 159,045 + GFF5529 0.31 -0.0 159,045 + GFF5529 0.31 -0.4 159,045 + GFF5529 0.31 -0.5 159,045 + GFF5529 0.31 +0.3 159,045 + GFF5529 0.31 +2.4 159,045 + GFF5529 0.31 +0.0 159,045 + GFF5529 0.31 -0.5 159,045 + GFF5529 0.31 +0.8 159,045 + GFF5529 0.31 -0.6 159,046 - GFF5529 0.31 +1.0 159,046 - GFF5529 0.31 +1.1 159,117 + GFF5529 0.40 -0.2 159,117 + GFF5529 0.40 +0.0 159,117 + GFF5529 0.40 +0.5 159,118 - GFF5529 0.40 -0.5 159,163 - GFF5529 0.46 +1.6 159,264 + GFF5529 0.58 +0.6 159,264 + GFF5529 0.58 -2.6 159,264 + GFF5529 0.58 +0.4 159,264 + GFF5529 0.58 -1.5 159,265 - GFF5529 0.58 +0.7 159,265 - GFF5529 0.58 -0.7 159,265 - GFF5529 0.58 +0.2 159,279 + GFF5529 0.60 -0.2 159,279 + GFF5529 0.60 +0.0 159,279 + GFF5529 0.60 -0.6 159,279 + GFF5529 0.60 -0.6 159,279 + GFF5529 0.60 -0.1 159,279 + GFF5529 0.60 -0.7 159,279 + GFF5529 0.60 +0.3 159,279 + GFF5529 0.60 -0.8 159,280 - GFF5529 0.60 +0.5 159,280 - GFF5529 0.60 -0.4 159,297 + GFF5529 0.62 +0.4 159,297 + GFF5529 0.62 +1.1 159,297 + GFF5529 0.62 -0.7 159,297 + GFF5529 0.62 +0.2 159,298 - GFF5529 0.62 -0.3 159,298 - GFF5529 0.62 -1.3 159,298 - GFF5529 0.62 -0.3 159,298 - GFF5529 0.62 +0.7 159,298 - GFF5529 0.62 -0.1 159,298 - GFF5529 0.62 +0.3 159,298 - GFF5529 0.62 -0.4 159,402 + GFF5529 0.75 -0.6 159,402 + GFF5529 0.75 -2.6 159,402 + GFF5529 0.75 -0.3 159,424 + GFF5529 0.78 +0.3 159,424 + GFF5529 0.78 -1.1 159,425 - GFF5529 0.78 +1.1 159,856 + GFF5530 0.23 -0.2 159,856 + GFF5530 0.23 -0.0 159,856 + GFF5530 0.23 -1.3 159,856 + GFF5530 0.23 +0.3 159,856 + GFF5530 0.23 -0.3 159,856 + GFF5530 0.23 -0.8 159,857 - GFF5530 0.23 +0.3 159,857 - GFF5530 0.23 -0.4 159,857 - GFF5530 0.23 -0.9 159,857 - GFF5530 0.23 -1.3 159,857 - GFF5530 0.23 +0.9 160,137 + GFF5530 0.50 -1.3 160,239 + GFF5530 0.60 +0.1 160,239 + GFF5530 0.60 -0.0 160,239 + GFF5530 0.60 +0.9 160,239 + GFF5530 0.60 -0.9 160,239 + GFF5530 0.60 -0.8 160,240 - GFF5530 0.60 +0.9 160,240 - GFF5530 0.60 -0.8 160,240 - GFF5530 0.60 +1.9 160,240 - GFF5530 0.60 -1.5 160,240 - GFF5530 0.60 +0.1 160,305 + GFF5530 0.66 +0.2 160,305 + GFF5530 0.66 +0.6 160,305 + GFF5530 0.66 -0.2 160,306 - GFF5530 0.66 -1.3 160,306 - GFF5530 0.66 +0.7 160,306 - GFF5530 0.66 -0.4 160,317 + GFF5530 0.67 -1.3 160,317 + GFF5530 0.67 +0.9 160,318 - GFF5530 0.67 +2.9 160,318 - GFF5530 0.67 -0.5 160,326 + GFF5530 0.68 +0.3 160,326 + GFF5530 0.68 -2.6 160,326 + GFF5530 0.68 -2.8 160,326 + GFF5530 0.68 +0.4 160,326 + GFF5530 0.68 +0.3 160,327 - GFF5530 0.68 -0.8 160,327 - GFF5530 0.68 -0.1 160,335 + GFF5530 0.69 -1.3 160,335 + GFF5530 0.69 -0.5 160,335 + GFF5530 0.69 +0.9 160,335 + GFF5530 0.69 -0.4 160,336 - GFF5530 0.69 -0.0 160,336 - GFF5530 0.69 +0.9 160,336 - GFF5530 0.69 -0.5 160,336 - GFF5530 0.69 -0.7 160,336 - GFF5530 0.69 -0.9 160,336 - GFF5530 0.69 -0.5 160,336 - GFF5530 0.69 -0.2 160,336 - GFF5530 0.69 -0.9 160,336 - GFF5530 0.69 +1.0 160,336 - GFF5530 0.69 -0.5 160,375 - GFF5530 0.73 -0.1 160,375 - GFF5530 0.73 -0.9 160,398 + GFF5530 0.75 +0.5 160,398 + GFF5530 0.75 +0.4 160,461 + GFF5530 0.81 -0.5 160,461 + GFF5530 0.81 -1.2 160,461 + GFF5530 0.81 -0.8 160,461 + GFF5530 0.81 +1.0 160,462 - GFF5530 0.81 -1.4 160,462 - GFF5530 0.81 -0.5 160,462 - GFF5530 0.81 -1.0 160,653 + +0.5 160,653 + +2.9 160,653 + +0.1 160,653 + -1.7 160,654 - -0.0 160,700 + -0.4 160,700 + +0.7 160,701 - -0.6 160,701 - +0.1 160,701 - +0.6 160,725 + +0.7 160,725 + -1.0 160,725 + +0.9 160,725 + +1.0 160,725 + -0.6 160,725 + -1.3 160,725 + -0.7 160,725 + +0.7 160,725 + -1.7 160,725 + -1.5 160,725 + -0.6 160,725 + -0.5 160,725 + -2.3 160,726 - -0.2 160,726 - -0.2 160,726 - -0.3 160,726 - -0.9 160,726 - -0.6 160,726 - -0.4 160,757 + -0.9 160,757 + -0.5 160,757 + -0.9 160,757 + -0.7 160,757 + -0.3 160,757 + +0.5 160,757 + -0.1 160,758 - +0.1 160,758 - -0.3 160,758 - -0.7 160,758 - +0.3 160,758 - -0.9 160,758 - -0.4 160,758 - +0.0 161,048 + GFF5531 0.72 -0.5 161,049 - GFF5531 0.72 -0.6 161,049 - GFF5531 0.72 -0.2 161,253 + +0.3 161,253 + -1.7 161,253 + -0.1 161,253 + +0.5 161,253 + +0.2 161,253 + -1.1 161,253 + +0.3 161,253 + -0.3 161,253 + +1.0 161,253 + -0.1 161,253 + +0.7 161,254 - -0.9 161,254 - -0.5 161,254 - -0.6 161,254 - -0.5 161,254 - -0.5 161,254 - +0.0 161,254 - -0.5 161,431 - GFF5532 0.26 +0.1 161,511 + GFF5532 0.36 +0.9 161,511 + GFF5532 0.36 -1.6 161,511 + GFF5532 0.36 -0.1 161,512 - GFF5532 0.36 +0.7 161,512 - GFF5532 0.36 -0.6 161,512 - GFF5532 0.36 +0.8 161,512 - GFF5532 0.36 +0.0 161,512 - GFF5532 0.36 -1.1 161,520 + GFF5532 0.37 -0.4
Or see this region's nucleotide sequence