Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5321 and GFF5322 overlap by 4 nucleotides GFF5322 and GFF5323 overlap by 4 nucleotides GFF5323 and GFF5324 are separated by 86 nucleotides GFF5324 and GFF5325 are separated by 34 nucleotides
GFF5321 - Type IV pilus biogenesis protein PilO, at 198,450 to 199,127
GFF5321
GFF5322 - Type IV pilus biogenesis protein PilP, at 199,124 to 199,663
GFF5322
GFF5323 - Type IV pilus biogenesis protein PilQ, at 199,660 to 201,840
GFF5323
GFF5324 - Shikimate kinase I (EC 2.7.1.71), at 201,927 to 202,412
GFF5324
GFF5325 - 3-dehydroquinate synthase (EC 4.2.3.4), at 202,447 to 203,556
GFF5325
Position (kb) 199
200
201
202 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 198.687 kb on + strand, within GFF5321 at 198.687 kb on + strand, within GFF5321 at 198.688 kb on - strand, within GFF5321 at 198.688 kb on - strand, within GFF5321 at 198.688 kb on - strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.756 kb on + strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.757 kb on - strand, within GFF5321 at 198.891 kb on + strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.892 kb on - strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.894 kb on + strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.895 kb on - strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.933 kb on + strand, within GFF5321 at 198.934 kb on - strand, within GFF5321 at 198.934 kb on - strand, within GFF5321 at 198.934 kb on - strand, within GFF5321 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.289 kb on + strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.290 kb on - strand, within GFF5322 at 199.508 kb on + strand, within GFF5322 at 199.508 kb on + strand, within GFF5322 at 199.508 kb on + strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.509 kb on - strand, within GFF5322 at 199.511 kb on + strand, within GFF5322 at 199.529 kb on + strand, within GFF5322 at 199.529 kb on + strand, within GFF5322 at 199.530 kb on - strand, within GFF5322 at 199.530 kb on - strand, within GFF5322 at 199.530 kb on - strand, within GFF5322 at 199.544 kb on + strand, within GFF5322 at 199.544 kb on + strand, within GFF5322 at 199.544 kb on + strand, within GFF5322 at 199.545 kb on - strand, within GFF5322 at 199.629 kb on + strand at 199.629 kb on + strand at 199.629 kb on + strand at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.011 kb on + strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.012 kb on - strand, within GFF5323 at 200.039 kb on + strand, within GFF5323 at 200.166 kb on + strand, within GFF5323 at 200.166 kb on + strand, within GFF5323 at 200.166 kb on + strand, within GFF5323 at 200.167 kb on - strand, within GFF5323 at 200.167 kb on - strand, within GFF5323 at 200.167 kb on - strand, within GFF5323 at 200.395 kb on + strand, within GFF5323 at 200.395 kb on + strand, within GFF5323 at 200.396 kb on - strand, within GFF5323 at 200.473 kb on + strand, within GFF5323 at 200.474 kb on - strand, within GFF5323 at 200.513 kb on - strand, within GFF5323 at 200.794 kb on + strand, within GFF5323 at 200.794 kb on + strand, within GFF5323 at 200.794 kb on + strand, within GFF5323 at 200.794 kb on + strand, within GFF5323 at 200.794 kb on + strand, within GFF5323 at 200.794 kb on + strand, within GFF5323 at 200.795 kb on - strand, within GFF5323 at 200.795 kb on - strand, within GFF5323 at 200.795 kb on - strand, within GFF5323 at 200.795 kb on - strand, within GFF5323 at 200.795 kb on - strand, within GFF5323 at 200.945 kb on + strand, within GFF5323 at 200.946 kb on - strand, within GFF5323 at 201.117 kb on + strand, within GFF5323 at 201.118 kb on - strand, within GFF5323 at 201.132 kb on + strand, within GFF5323 at 201.132 kb on + strand, within GFF5323 at 201.279 kb on + strand, within GFF5323 at 201.316 kb on + strand, within GFF5323 at 201.384 kb on - strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.546 kb on + strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.547 kb on - strand, within GFF5323 at 201.675 kb on + strand at 201.675 kb on + strand at 201.675 kb on + strand at 201.675 kb on + strand at 201.676 kb on - strand at 201.676 kb on - strand at 201.676 kb on - strand at 201.737 kb on - strand at 201.737 kb on - strand at 201.784 kb on + strand at 201.798 kb on + strand at 201.798 kb on + strand at 201.799 kb on - strand at 201.817 kb on - strand at 201.852 kb on - strand at 201.999 kb on + strand, within GFF5324 at 201.999 kb on + strand, within GFF5324 at 202.155 kb on + strand, within GFF5324 at 202.155 kb on + strand, within GFF5324 at 202.278 kb on + strand, within GFF5324 at 202.460 kb on + strand at 202.460 kb on + strand at 202.461 kb on - strand at 202.461 kb on - strand at 202.461 kb on - strand at 202.618 kb on + strand, within GFF5325 at 202.826 kb on - strand, within GFF5325 at 202.826 kb on - strand, within GFF5325
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 198,687 + GFF5321 0.35 -0.5 198,687 + GFF5321 0.35 -1.1 198,688 - GFF5321 0.35 -0.6 198,688 - GFF5321 0.35 -0.2 198,688 - GFF5321 0.35 +0.2 198,756 + GFF5321 0.45 -0.9 198,756 + GFF5321 0.45 -2.6 198,756 + GFF5321 0.45 -0.9 198,756 + GFF5321 0.45 -0.7 198,756 + GFF5321 0.45 -1.6 198,756 + GFF5321 0.45 -1.1 198,757 - GFF5321 0.45 -0.9 198,757 - GFF5321 0.45 -0.5 198,757 - GFF5321 0.45 -0.8 198,757 - GFF5321 0.45 -0.8 198,757 - GFF5321 0.45 -1.3 198,757 - GFF5321 0.45 -2.4 198,757 - GFF5321 0.45 -2.0 198,757 - GFF5321 0.45 -1.4 198,757 - GFF5321 0.45 -1.8 198,891 + GFF5321 0.65 -2.1 198,892 - GFF5321 0.65 -0.5 198,892 - GFF5321 0.65 -0.8 198,892 - GFF5321 0.65 -2.5 198,892 - GFF5321 0.65 -2.4 198,892 - GFF5321 0.65 -2.4 198,892 - GFF5321 0.65 -1.4 198,892 - GFF5321 0.65 +0.5 198,892 - GFF5321 0.65 -1.4 198,892 - GFF5321 0.65 -1.2 198,894 + GFF5321 0.65 -1.5 198,894 + GFF5321 0.65 -0.9 198,894 + GFF5321 0.65 -1.2 198,894 + GFF5321 0.65 -2.1 198,894 + GFF5321 0.65 +1.5 198,894 + GFF5321 0.65 -1.0 198,894 + GFF5321 0.65 -0.3 198,894 + GFF5321 0.65 -1.4 198,894 + GFF5321 0.65 -1.1 198,895 - GFF5321 0.66 -0.1 198,895 - GFF5321 0.66 -0.3 198,895 - GFF5321 0.66 -1.1 198,895 - GFF5321 0.66 -1.1 198,895 - GFF5321 0.66 -0.4 198,895 - GFF5321 0.66 -1.2 198,895 - GFF5321 0.66 -0.7 198,895 - GFF5321 0.66 +0.7 198,895 - GFF5321 0.66 -1.6 198,933 + GFF5321 0.71 -1.3 198,933 + GFF5321 0.71 -1.8 198,933 + GFF5321 0.71 -1.7 198,933 + GFF5321 0.71 -1.1 198,933 + GFF5321 0.71 -1.5 198,934 - GFF5321 0.71 -1.1 198,934 - GFF5321 0.71 -0.1 198,934 - GFF5321 0.71 -1.6 199,289 + GFF5322 0.31 -0.9 199,289 + GFF5322 0.31 -1.0 199,289 + GFF5322 0.31 -1.6 199,289 + GFF5322 0.31 -2.6 199,289 + GFF5322 0.31 -2.2 199,289 + GFF5322 0.31 -1.3 199,289 + GFF5322 0.31 -1.9 199,289 + GFF5322 0.31 -1.0 199,289 + GFF5322 0.31 -0.3 199,290 - GFF5322 0.31 -2.3 199,290 - GFF5322 0.31 -0.7 199,290 - GFF5322 0.31 -0.3 199,290 - GFF5322 0.31 -1.0 199,290 - GFF5322 0.31 +0.1 199,508 + GFF5322 0.71 +1.1 199,508 + GFF5322 0.71 -2.5 199,508 + GFF5322 0.71 -1.3 199,509 - GFF5322 0.71 -2.6 199,509 - GFF5322 0.71 -1.1 199,509 - GFF5322 0.71 -1.1 199,509 - GFF5322 0.71 -2.4 199,509 - GFF5322 0.71 -2.1 199,509 - GFF5322 0.71 -0.2 199,509 - GFF5322 0.71 -0.3 199,511 + GFF5322 0.72 -2.1 199,529 + GFF5322 0.75 -2.3 199,529 + GFF5322 0.75 -0.9 199,530 - GFF5322 0.75 -0.3 199,530 - GFF5322 0.75 -1.8 199,530 - GFF5322 0.75 -1.6 199,544 + GFF5322 0.78 -0.9 199,544 + GFF5322 0.78 -0.8 199,544 + GFF5322 0.78 -0.7 199,545 - GFF5322 0.78 -0.6 199,629 + -1.6 199,629 + -1.4 199,629 + -1.2 200,011 + GFF5323 0.16 +0.2 200,011 + GFF5323 0.16 -0.2 200,011 + GFF5323 0.16 -0.6 200,011 + GFF5323 0.16 +0.1 200,011 + GFF5323 0.16 -2.3 200,011 + GFF5323 0.16 -1.2 200,011 + GFF5323 0.16 -0.9 200,011 + GFF5323 0.16 -0.6 200,012 - GFF5323 0.16 -1.3 200,012 - GFF5323 0.16 -2.5 200,012 - GFF5323 0.16 -0.3 200,012 - GFF5323 0.16 -1.8 200,012 - GFF5323 0.16 -0.5 200,012 - GFF5323 0.16 -1.2 200,012 - GFF5323 0.16 -0.9 200,012 - GFF5323 0.16 -0.6 200,012 - GFF5323 0.16 -2.0 200,012 - GFF5323 0.16 -0.7 200,039 + GFF5323 0.17 -0.7 200,166 + GFF5323 0.23 -1.1 200,166 + GFF5323 0.23 -2.6 200,166 + GFF5323 0.23 -2.3 200,167 - GFF5323 0.23 -1.6 200,167 - GFF5323 0.23 -0.0 200,167 - GFF5323 0.23 -2.4 200,395 + GFF5323 0.34 -0.6 200,395 + GFF5323 0.34 -0.1 200,396 - GFF5323 0.34 -0.1 200,473 + GFF5323 0.37 -0.6 200,474 - GFF5323 0.37 -0.6 200,513 - GFF5323 0.39 -0.7 200,794 + GFF5323 0.52 -1.3 200,794 + GFF5323 0.52 -1.7 200,794 + GFF5323 0.52 -1.8 200,794 + GFF5323 0.52 -1.8 200,794 + GFF5323 0.52 -1.3 200,794 + GFF5323 0.52 -1.2 200,795 - GFF5323 0.52 -0.6 200,795 - GFF5323 0.52 -1.1 200,795 - GFF5323 0.52 -0.3 200,795 - GFF5323 0.52 -1.3 200,795 - GFF5323 0.52 -0.8 200,945 + GFF5323 0.59 -0.4 200,946 - GFF5323 0.59 -1.5 201,117 + GFF5323 0.67 -1.6 201,118 - GFF5323 0.67 -2.6 201,132 + GFF5323 0.67 -0.4 201,132 + GFF5323 0.67 -1.5 201,279 + GFF5323 0.74 -1.3 201,316 + GFF5323 0.76 -1.6 201,384 - GFF5323 0.79 -1.8 201,546 + GFF5323 0.86 -1.0 201,546 + GFF5323 0.86 -0.3 201,546 + GFF5323 0.86 +0.1 201,546 + GFF5323 0.86 -1.8 201,546 + GFF5323 0.86 -2.4 201,546 + GFF5323 0.86 +0.4 201,546 + GFF5323 0.86 -1.4 201,546 + GFF5323 0.86 -2.5 201,546 + GFF5323 0.86 -1.7 201,547 - GFF5323 0.87 -1.7 201,547 - GFF5323 0.87 -0.6 201,547 - GFF5323 0.87 -0.1 201,547 - GFF5323 0.87 -1.4 201,547 - GFF5323 0.87 +0.1 201,547 - GFF5323 0.87 -2.6 201,547 - GFF5323 0.87 -1.5 201,547 - GFF5323 0.87 +0.5 201,547 - GFF5323 0.87 -0.8 201,675 + -1.1 201,675 + -0.1 201,675 + +0.3 201,675 + -0.8 201,676 - -1.5 201,676 - -0.3 201,676 - -1.6 201,737 - -3.6 201,737 - -4.7 201,784 + -2.0 201,798 + -4.2 201,798 + -3.2 201,799 - -3.6 201,817 - -1.3 201,852 - -0.1 201,999 + GFF5324 0.15 -1.4 201,999 + GFF5324 0.15 -0.6 202,155 + GFF5324 0.47 -0.6 202,155 + GFF5324 0.47 -3.2 202,278 + GFF5324 0.72 -0.6 202,460 + -0.3 202,460 + -0.5 202,461 - -1.2 202,461 - -0.7 202,461 - -0.2 202,618 + GFF5325 0.15 -0.9 202,826 - GFF5325 0.34 -0.6 202,826 - GFF5325 0.34 -1.1
Or see this region's nucleotide sequence