Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5309 and GFF5310 are separated by 73 nucleotides GFF5310 and GFF5311 are separated by 166 nucleotides GFF5311 and GFF5312 overlap by 4 nucleotides
GFF5309 - GTP-binding protein EngB, at 185,231 to 185,971
GFF5309
GFF5310 - Cytochrome c4, at 186,045 to 186,725
GFF5310
GFF5311 - Cytochrome c-type biogenesis protein Ccs1/ResB, at 186,892 to 189,012
GFF5311
GFF5312 - Cytochrome c-type biogenesis protein CcsA/ResC, at 189,009 to 190,325
GFF5312
Position (kb) 186
187
188
189
190 Strain fitness (log2 ratio) -2
-1
0
1 at 186.003 kb on - strand at 186.003 kb on - strand at 186.045 kb on - strand at 186.062 kb on + strand at 186.062 kb on + strand at 186.062 kb on + strand at 186.062 kb on + strand at 186.062 kb on + strand at 186.063 kb on - strand at 186.063 kb on - strand at 186.063 kb on - strand at 186.063 kb on - strand at 186.063 kb on - strand at 186.182 kb on + strand, within GFF5310 at 186.182 kb on + strand, within GFF5310 at 186.182 kb on + strand, within GFF5310 at 186.182 kb on + strand, within GFF5310 at 186.182 kb on + strand, within GFF5310 at 186.182 kb on + strand, within GFF5310 at 186.183 kb on - strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.294 kb on + strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.295 kb on - strand, within GFF5310 at 186.474 kb on + strand, within GFF5310 at 186.475 kb on - strand, within GFF5310 at 186.556 kb on - strand, within GFF5310 at 186.556 kb on - strand, within GFF5310 at 186.585 kb on + strand, within GFF5310 at 186.585 kb on + strand, within GFF5310 at 186.585 kb on + strand, within GFF5310 at 186.585 kb on + strand, within GFF5310 at 186.585 kb on + strand, within GFF5310 at 186.586 kb on - strand, within GFF5310 at 186.586 kb on - strand, within GFF5310 at 186.586 kb on - strand, within GFF5310 at 186.586 kb on - strand, within GFF5310 at 186.586 kb on - strand, within GFF5310 at 186.586 kb on - strand, within GFF5310 at 186.705 kb on + strand at 186.705 kb on + strand at 186.705 kb on + strand at 186.705 kb on + strand at 186.705 kb on + strand at 186.706 kb on - strand at 186.706 kb on - strand at 186.706 kb on - strand at 186.706 kb on - strand at 186.706 kb on - strand at 186.706 kb on - strand at 186.706 kb on - strand at 186.751 kb on + strand at 186.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 186,003 - +1.1 186,003 - -0.8 186,045 - -0.7 186,062 + +1.0 186,062 + -1.0 186,062 + -2.0 186,062 + -0.9 186,062 + -0.5 186,063 - +0.7 186,063 - -1.4 186,063 - -0.9 186,063 - -1.6 186,063 - -0.6 186,182 + GFF5310 0.20 -0.4 186,182 + GFF5310 0.20 -0.7 186,182 + GFF5310 0.20 -1.0 186,182 + GFF5310 0.20 -0.7 186,182 + GFF5310 0.20 -0.5 186,182 + GFF5310 0.20 +0.7 186,183 - GFF5310 0.20 -1.3 186,294 + GFF5310 0.37 -0.9 186,294 + GFF5310 0.37 -1.2 186,294 + GFF5310 0.37 +0.4 186,294 + GFF5310 0.37 -1.7 186,294 + GFF5310 0.37 -1.1 186,294 + GFF5310 0.37 -1.6 186,294 + GFF5310 0.37 -0.6 186,294 + GFF5310 0.37 +1.1 186,294 + GFF5310 0.37 -1.1 186,294 + GFF5310 0.37 -1.6 186,294 + GFF5310 0.37 -1.3 186,294 + GFF5310 0.37 -0.7 186,294 + GFF5310 0.37 -0.3 186,295 - GFF5310 0.37 -1.3 186,295 - GFF5310 0.37 -1.3 186,295 - GFF5310 0.37 -0.4 186,295 - GFF5310 0.37 -1.0 186,295 - GFF5310 0.37 -2.5 186,295 - GFF5310 0.37 +0.1 186,295 - GFF5310 0.37 +0.5 186,295 - GFF5310 0.37 +0.1 186,295 - GFF5310 0.37 -0.9 186,474 + GFF5310 0.63 -0.9 186,475 - GFF5310 0.63 +0.0 186,556 - GFF5310 0.75 -1.1 186,556 - GFF5310 0.75 -2.5 186,585 + GFF5310 0.79 -1.9 186,585 + GFF5310 0.79 -1.2 186,585 + GFF5310 0.79 -2.4 186,585 + GFF5310 0.79 -0.9 186,585 + GFF5310 0.79 -0.8 186,586 - GFF5310 0.79 +0.8 186,586 - GFF5310 0.79 -2.5 186,586 - GFF5310 0.79 -2.6 186,586 - GFF5310 0.79 -1.4 186,586 - GFF5310 0.79 +0.2 186,586 - GFF5310 0.79 -1.5 186,705 + -1.5 186,705 + -1.8 186,705 + -0.7 186,705 + -0.9 186,705 + -1.1 186,706 - -0.2 186,706 - -1.0 186,706 - -0.7 186,706 - -1.3 186,706 - -1.0 186,706 - -2.0 186,706 - -0.3 186,751 + +0.4 186,833 + -0.2
Or see this region's nucleotide sequence