Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5147 and GFF5148 are separated by 25 nucleotides
GFF5147 - Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17), at 309 to 1,205
GFF5147
GFF5148 - Taurine ABC transporter, substrate-binding protein TauA, at 1,231 to 2,241
GFF5148
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 0.151 kb on + strand at 0.152 kb on - strand at 0.152 kb on - strand at 0.180 kb on - strand at 0.180 kb on - strand at 0.180 kb on - strand at 0.242 kb on - strand at 0.242 kb on - strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.251 kb on + strand at 0.252 kb on - strand at 0.252 kb on - strand at 0.252 kb on - strand at 0.252 kb on - strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.256 kb on + strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.257 kb on - strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.296 kb on + strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.297 kb on - strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.367 kb on + strand at 0.368 kb on - strand at 0.368 kb on - strand at 0.368 kb on - strand at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.409 kb on + strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.410 kb on - strand, within GFF5147 at 0.484 kb on + strand, within GFF5147 at 0.485 kb on - strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.657 kb on + strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.658 kb on - strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.678 kb on + strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.679 kb on - strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.831 kb on + strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.832 kb on - strand, within GFF5147 at 0.843 kb on + strand, within GFF5147 at 0.843 kb on + strand, within GFF5147 at 0.843 kb on + strand, within GFF5147 at 0.844 kb on - strand, within GFF5147 at 0.844 kb on - strand, within GFF5147 at 0.844 kb on - strand, within GFF5147 at 1.071 kb on + strand, within GFF5147 at 1.072 kb on - strand, within GFF5147 at 1.348 kb on + strand, within GFF5148 at 1.348 kb on + strand, within GFF5148 at 1.586 kb on - strand, within GFF5148 at 1.586 kb on - strand, within GFF5148 at 1.588 kb on + strand, within GFF5148 at 1.588 kb on + strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.589 kb on - strand, within GFF5148 at 1.888 kb on + strand, within GFF5148 at 1.888 kb on + strand, within GFF5148 at 1.888 kb on + strand, within GFF5148 at 1.889 kb on - strand, within GFF5148 at 1.889 kb on - strand, within GFF5148 at 1.928 kb on - strand, within GFF5148 at 1.928 kb on - strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.966 kb on + strand, within GFF5148 at 1.967 kb on - strand, within GFF5148 at 1.967 kb on - strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.041 kb on + strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.042 kb on - strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.137 kb on + strand, within GFF5148 at 2.138 kb on - strand, within GFF5148 at 2.138 kb on - strand, within GFF5148 at 2.138 kb on - strand, within GFF5148 at 2.203 kb on + strand at 2.203 kb on + strand at 2.203 kb on + strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand at 2.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 151 + -1.8 152 - -0.2 152 - -1.7 180 - -1.2 180 - -0.6 180 - +0.5 242 - -0.1 242 - +0.4 251 + +0.2 251 + +1.7 251 + -0.1 251 + +0.6 251 + -0.3 251 + -0.6 251 + -1.2 251 + -0.3 252 - +0.2 252 - +0.3 252 - +1.1 252 - -1.5 256 + -0.3 256 + -0.3 256 + +0.5 256 + +0.3 256 + -0.0 256 + -0.4 256 + -1.3 256 + +0.1 256 + +1.6 256 + +0.2 256 + +1.1 257 - +0.2 257 - +0.1 257 - -0.2 257 - +0.7 257 - -1.0 257 - +0.0 257 - -1.4 257 - -0.6 257 - +0.9 296 + -0.3 296 + -0.8 296 + -0.4 296 + -1.7 296 + -0.6 296 + -0.2 296 + -0.0 296 + -0.2 296 + -1.1 296 + -0.4 296 + -0.3 296 + +0.5 296 + -0.6 296 + +0.6 296 + -0.1 296 + -1.3 297 - +0.1 297 - -0.3 297 - +0.1 297 - -1.2 297 - +0.1 297 - +1.1 297 - +0.1 297 - -1.7 297 - +1.4 297 - +0.0 367 + -0.2 367 + -0.2 367 + -1.2 367 + -0.8 367 + +0.1 368 - +0.2 368 - +0.6 368 - -0.6 409 + GFF5147 0.11 +0.3 409 + GFF5147 0.11 +0.2 409 + GFF5147 0.11 +0.1 409 + GFF5147 0.11 +1.4 409 + GFF5147 0.11 -3.0 410 - GFF5147 0.11 +0.1 410 - GFF5147 0.11 +0.2 410 - GFF5147 0.11 -0.2 410 - GFF5147 0.11 -1.3 410 - GFF5147 0.11 -1.8 410 - GFF5147 0.11 -0.4 484 + GFF5147 0.20 -1.0 485 - GFF5147 0.20 +0.3 657 + GFF5147 0.39 -0.2 657 + GFF5147 0.39 -0.1 657 + GFF5147 0.39 -0.3 657 + GFF5147 0.39 -2.4 657 + GFF5147 0.39 +0.1 658 - GFF5147 0.39 -0.7 658 - GFF5147 0.39 -0.6 658 - GFF5147 0.39 +0.1 658 - GFF5147 0.39 +0.0 658 - GFF5147 0.39 +0.1 658 - GFF5147 0.39 +0.7 678 + GFF5147 0.41 +1.2 678 + GFF5147 0.41 -0.2 678 + GFF5147 0.41 +0.4 678 + GFF5147 0.41 -0.0 679 - GFF5147 0.41 +0.1 679 - GFF5147 0.41 +1.4 679 - GFF5147 0.41 +0.4 679 - GFF5147 0.41 +0.9 679 - GFF5147 0.41 +0.1 679 - GFF5147 0.41 -0.5 679 - GFF5147 0.41 -0.1 831 + GFF5147 0.58 -0.5 831 + GFF5147 0.58 -0.5 831 + GFF5147 0.58 +0.1 831 + GFF5147 0.58 -0.9 831 + GFF5147 0.58 +0.1 831 + GFF5147 0.58 +0.2 831 + GFF5147 0.58 +0.5 831 + GFF5147 0.58 -0.1 831 + GFF5147 0.58 +0.8 831 + GFF5147 0.58 -0.9 831 + GFF5147 0.58 -0.3 832 - GFF5147 0.58 -0.0 832 - GFF5147 0.58 +0.3 832 - GFF5147 0.58 +1.1 832 - GFF5147 0.58 -0.5 832 - GFF5147 0.58 -0.3 832 - GFF5147 0.58 +0.7 832 - GFF5147 0.58 +1.7 832 - GFF5147 0.58 -1.5 843 + GFF5147 0.60 -0.5 843 + GFF5147 0.60 -0.8 843 + GFF5147 0.60 -0.6 844 - GFF5147 0.60 -0.9 844 - GFF5147 0.60 +0.5 844 - GFF5147 0.60 +0.0 1,071 + GFF5147 0.85 -0.2 1,072 - GFF5147 0.85 -0.1 1,348 + GFF5148 0.12 +0.2 1,348 + GFF5148 0.12 -0.1 1,586 - GFF5148 0.35 +0.2 1,586 - GFF5148 0.35 -0.9 1,588 + GFF5148 0.35 -0.1 1,588 + GFF5148 0.35 -0.2 1,589 - GFF5148 0.35 +0.5 1,589 - GFF5148 0.35 +0.1 1,589 - GFF5148 0.35 -0.3 1,589 - GFF5148 0.35 +0.1 1,888 + GFF5148 0.65 -1.7 1,888 + GFF5148 0.65 -0.2 1,888 + GFF5148 0.65 -0.8 1,889 - GFF5148 0.65 -0.8 1,889 - GFF5148 0.65 +0.2 1,928 - GFF5148 0.69 +0.4 1,928 - GFF5148 0.69 +2.3 1,966 + GFF5148 0.73 +0.2 1,966 + GFF5148 0.73 -0.0 1,966 + GFF5148 0.73 -0.2 1,966 + GFF5148 0.73 +0.7 1,966 + GFF5148 0.73 +0.4 1,967 - GFF5148 0.73 +0.1 1,967 - GFF5148 0.73 -0.7 2,041 + GFF5148 0.80 -0.4 2,041 + GFF5148 0.80 -0.7 2,041 + GFF5148 0.80 -0.1 2,041 + GFF5148 0.80 +1.0 2,041 + GFF5148 0.80 +0.0 2,041 + GFF5148 0.80 -0.0 2,041 + GFF5148 0.80 -0.4 2,041 + GFF5148 0.80 +0.1 2,041 + GFF5148 0.80 -0.1 2,041 + GFF5148 0.80 +0.5 2,041 + GFF5148 0.80 -0.8 2,042 - GFF5148 0.80 -0.4 2,042 - GFF5148 0.80 -0.5 2,042 - GFF5148 0.80 +0.1 2,042 - GFF5148 0.80 -0.5 2,042 - GFF5148 0.80 -0.8 2,042 - GFF5148 0.80 -2.8 2,042 - GFF5148 0.80 +0.9 2,042 - GFF5148 0.80 +0.1 2,042 - GFF5148 0.80 -0.7 2,137 + GFF5148 0.90 -0.3 2,137 + GFF5148 0.90 +0.1 2,137 + GFF5148 0.90 +0.2 2,137 + GFF5148 0.90 +0.0 2,137 + GFF5148 0.90 +2.3 2,137 + GFF5148 0.90 +1.7 2,137 + GFF5148 0.90 -0.7 2,138 - GFF5148 0.90 +0.1 2,138 - GFF5148 0.90 -0.6 2,138 - GFF5148 0.90 -0.1 2,203 + -1.7 2,203 + +0.2 2,203 + +0.1 2,204 - +0.6 2,204 - -0.1 2,204 - +0.5 2,204 - -0.7 2,204 - -1.4 2,204 - -0.5 2,204 - -0.0
Or see this region's nucleotide sequence