Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5054 and GFF5055 overlap by 4 nucleotides GFF5055 and GFF5056 are separated by 22 nucleotides
GFF5054 - Homogentisate 1,2-dioxygenase (EC 1.13.11.5), at 154,809 to 156,119
GFF5054
GFF5055 - BUG/TctC family periplasmic protein, at 156,116 to 157,090
GFF5055
GFF5056 - Fumarylacetoacetase (EC 3.7.1.2), at 157,113 to 158,378
GFF5056
Position (kb)
156
157
158 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.223 kb on + strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.224 kb on - strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.415 kb on + strand, within GFF5054 at 155.416 kb on - strand, within GFF5054 at 155.430 kb on + strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.431 kb on - strand, within GFF5054 at 155.541 kb on + strand, within GFF5054 at 155.542 kb on - strand, within GFF5054 at 155.644 kb on - strand, within GFF5054 at 155.841 kb on + strand, within GFF5054 at 155.862 kb on + strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 155.863 kb on - strand, within GFF5054 at 156.075 kb on + strand at 156.075 kb on + strand at 156.076 kb on - strand at 156.198 kb on - strand at 156.198 kb on - strand at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.230 kb on + strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.231 kb on - strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.380 kb on + strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.381 kb on - strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.431 kb on + strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.432 kb on - strand, within GFF5055 at 156.581 kb on + strand, within GFF5055 at 156.581 kb on + strand, within GFF5055 at 156.582 kb on - strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.668 kb on + strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.669 kb on - strand, within GFF5055 at 156.755 kb on - strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.881 kb on + strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 156.882 kb on - strand, within GFF5055 at 157.040 kb on + strand at 157.040 kb on + strand at 157.040 kb on + strand at 157.041 kb on - strand at 157.041 kb on - strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.108 kb on + strand at 157.109 kb on - strand at 157.109 kb on - strand at 157.109 kb on - strand at 157.476 kb on + strand, within GFF5056 at 157.476 kb on + strand, within GFF5056 at 157.476 kb on + strand, within GFF5056 at 157.477 kb on - strand, within GFF5056 at 157.477 kb on - strand, within GFF5056 at 157.477 kb on - strand, within GFF5056 at 157.477 kb on - strand, within GFF5056 at 157.477 kb on - strand, within GFF5056 at 157.477 kb on - strand, within GFF5056 at 157.488 kb on + strand, within GFF5056 at 157.488 kb on + strand, within GFF5056 at 157.488 kb on + strand, within GFF5056 at 157.489 kb on - strand, within GFF5056 at 157.489 kb on - strand, within GFF5056 at 157.489 kb on - strand, within GFF5056 at 157.489 kb on - strand, within GFF5056 at 157.489 kb on - strand, within GFF5056 at 157.489 kb on - strand, within GFF5056 at 157.489 kb on - strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.696 kb on - strand, within GFF5056 at 157.696 kb on - strand, within GFF5056 at 157.833 kb on + strand, within GFF5056 at 157.833 kb on + strand, within GFF5056 at 157.833 kb on + strand, within GFF5056 at 157.834 kb on - strand, within GFF5056 at 157.834 kb on - strand, within GFF5056 at 157.834 kb on - strand, within GFF5056
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 155,223 + GFF5054 0.32 +0.5 155,223 + GFF5054 0.32 -0.2 155,223 + GFF5054 0.32 +0.3 155,223 + GFF5054 0.32 -0.2 155,223 + GFF5054 0.32 -1.2 155,223 + GFF5054 0.32 -0.3 155,224 - GFF5054 0.32 +1.3 155,224 - GFF5054 0.32 -0.4 155,224 - GFF5054 0.32 -0.1 155,224 - GFF5054 0.32 -0.6 155,224 - GFF5054 0.32 -0.2 155,224 - GFF5054 0.32 -0.0 155,224 - GFF5054 0.32 +0.7 155,415 + GFF5054 0.46 -0.1 155,415 + GFF5054 0.46 -0.2 155,416 - GFF5054 0.46 -0.7 155,430 + GFF5054 0.47 +0.4 155,431 - GFF5054 0.47 -1.0 155,431 - GFF5054 0.47 -1.4 155,431 - GFF5054 0.47 -0.5 155,431 - GFF5054 0.47 +0.1 155,541 + GFF5054 0.56 +0.2 155,542 - GFF5054 0.56 -0.2 155,644 - GFF5054 0.64 +0.4 155,841 + GFF5054 0.79 -0.0 155,862 + GFF5054 0.80 -0.4 155,863 - GFF5054 0.80 +0.1 155,863 - GFF5054 0.80 -1.5 155,863 - GFF5054 0.80 -0.3 155,863 - GFF5054 0.80 +1.0 156,075 + -2.2 156,075 + -0.4 156,076 - +0.3 156,198 - -0.9 156,198 - +0.8 156,230 + GFF5055 0.12 -0.2 156,230 + GFF5055 0.12 +0.2 156,230 + GFF5055 0.12 -0.3 156,230 + GFF5055 0.12 -0.3 156,230 + GFF5055 0.12 -0.4 156,230 + GFF5055 0.12 +0.0 156,231 - GFF5055 0.12 +0.0 156,231 - GFF5055 0.12 +0.5 156,231 - GFF5055 0.12 -2.0 156,231 - GFF5055 0.12 -0.5 156,380 + GFF5055 0.27 +0.7 156,380 + GFF5055 0.27 -0.8 156,380 + GFF5055 0.27 +0.0 156,381 - GFF5055 0.27 +0.6 156,381 - GFF5055 0.27 +1.3 156,381 - GFF5055 0.27 +0.6 156,381 - GFF5055 0.27 +0.8 156,381 - GFF5055 0.27 +0.3 156,381 - GFF5055 0.27 -0.4 156,431 + GFF5055 0.32 +1.4 156,431 + GFF5055 0.32 -1.1 156,431 + GFF5055 0.32 -1.6 156,431 + GFF5055 0.32 +1.6 156,431 + GFF5055 0.32 -1.9 156,431 + GFF5055 0.32 +0.3 156,431 + GFF5055 0.32 +1.3 156,431 + GFF5055 0.32 -0.7 156,432 - GFF5055 0.32 -0.7 156,432 - GFF5055 0.32 +0.8 156,432 - GFF5055 0.32 -1.2 156,432 - GFF5055 0.32 -0.1 156,432 - GFF5055 0.32 -0.1 156,432 - GFF5055 0.32 -1.6 156,432 - GFF5055 0.32 +0.3 156,432 - GFF5055 0.32 +0.1 156,581 + GFF5055 0.48 +0.1 156,581 + GFF5055 0.48 +0.8 156,582 - GFF5055 0.48 -0.4 156,668 + GFF5055 0.57 -0.4 156,668 + GFF5055 0.57 +0.1 156,668 + GFF5055 0.57 +0.9 156,668 + GFF5055 0.57 +0.4 156,668 + GFF5055 0.57 -0.5 156,668 + GFF5055 0.57 -2.2 156,668 + GFF5055 0.57 +1.8 156,668 + GFF5055 0.57 +0.5 156,669 - GFF5055 0.57 +0.8 156,669 - GFF5055 0.57 +0.7 156,669 - GFF5055 0.57 +1.4 156,669 - GFF5055 0.57 +0.7 156,669 - GFF5055 0.57 +1.7 156,755 - GFF5055 0.66 -0.4 156,881 + GFF5055 0.78 +0.1 156,881 + GFF5055 0.78 -0.2 156,881 + GFF5055 0.78 -2.3 156,882 - GFF5055 0.79 +0.1 156,882 - GFF5055 0.79 +0.4 156,882 - GFF5055 0.79 -0.4 156,882 - GFF5055 0.79 -0.4 157,040 + -0.0 157,040 + -0.6 157,040 + -1.2 157,041 - -0.5 157,041 - +0.1 157,108 + -0.3 157,108 + +1.8 157,108 + -0.7 157,108 + +0.0 157,108 + -0.5 157,108 + -0.8 157,108 + -0.3 157,109 - +0.0 157,109 - -1.2 157,109 - -0.3 157,476 + GFF5056 0.29 +0.5 157,476 + GFF5056 0.29 +0.5 157,476 + GFF5056 0.29 -1.8 157,477 - GFF5056 0.29 -0.7 157,477 - GFF5056 0.29 -2.0 157,477 - GFF5056 0.29 -0.9 157,477 - GFF5056 0.29 -0.2 157,477 - GFF5056 0.29 -1.5 157,477 - GFF5056 0.29 +0.0 157,488 + GFF5056 0.30 +0.5 157,488 + GFF5056 0.30 +0.1 157,488 + GFF5056 0.30 -0.7 157,489 - GFF5056 0.30 +0.3 157,489 - GFF5056 0.30 -1.0 157,489 - GFF5056 0.30 -0.4 157,489 - GFF5056 0.30 -1.7 157,489 - GFF5056 0.30 +0.8 157,489 - GFF5056 0.30 -1.3 157,489 - GFF5056 0.30 +1.1 157,557 + GFF5056 0.35 -0.7 157,557 + GFF5056 0.35 +0.7 157,557 + GFF5056 0.35 -0.5 157,557 + GFF5056 0.35 +0.5 157,557 + GFF5056 0.35 -1.0 157,557 + GFF5056 0.35 +0.5 157,557 + GFF5056 0.35 +0.6 157,558 - GFF5056 0.35 +1.3 157,558 - GFF5056 0.35 +0.5 157,558 - GFF5056 0.35 +1.4 157,558 - GFF5056 0.35 -1.5 157,558 - GFF5056 0.35 -0.9 157,558 - GFF5056 0.35 +0.8 157,558 - GFF5056 0.35 +0.3 157,558 - GFF5056 0.35 -0.9 157,558 - GFF5056 0.35 -0.0 157,558 - GFF5056 0.35 +1.0 157,558 - GFF5056 0.35 +0.4 157,558 - GFF5056 0.35 +0.5 157,558 - GFF5056 0.35 +0.2 157,558 - GFF5056 0.35 -0.1 157,558 - GFF5056 0.35 +0.2 157,558 - GFF5056 0.35 -0.3 157,558 - GFF5056 0.35 -0.2 157,558 - GFF5056 0.35 -0.6 157,695 + GFF5056 0.46 +0.3 157,695 + GFF5056 0.46 -1.9 157,695 + GFF5056 0.46 +0.3 157,695 + GFF5056 0.46 +1.1 157,696 - GFF5056 0.46 -2.4 157,696 - GFF5056 0.46 +1.1 157,833 + GFF5056 0.57 -1.3 157,833 + GFF5056 0.57 +0.2 157,833 + GFF5056 0.57 -1.0 157,834 - GFF5056 0.57 -0.1 157,834 - GFF5056 0.57 -1.2 157,834 - GFF5056 0.57 -1.4
Or see this region's nucleotide sequence