Strain Fitness in Variovorax sp. SCN45 around GFF499

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF498 and GFF499 overlap by 4 nucleotidesGFF499 and GFF500 are separated by 16 nucleotidesGFF500 and GFF501 are separated by 119 nucleotides GFF498 - Squalene/phytoene desaturase HopC, at 542,122 to 543,378 GFF498 GFF499 - hypothetical protein, at 543,375 to 543,683 GFF499 GFF500 - Lysine exporter protein (LYSE/YGGA), at 543,700 to 544,344 GFF500 GFF501 - hypothetical protein, at 544,464 to 544,769 GFF501 Position (kb) 543 544Strain fitness (log2 ratio) -2 -1 0 1 2at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.980 kb on + strand, within GFF498at 542.981 kb on - strand, within GFF498at 542.981 kb on - strand, within GFF498at 542.981 kb on - strand, within GFF498at 542.981 kb on - strand, within GFF498at 543.095 kb on + strand, within GFF498at 543.298 kb on + strandat 543.393 kb on + strandat 543.394 kb on - strandat 543.394 kb on - strandat 543.409 kb on - strand, within GFF499at 543.409 kb on - strand, within GFF499at 543.481 kb on + strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.482 kb on - strand, within GFF499at 543.570 kb on + strand, within GFF499at 543.570 kb on + strand, within GFF499at 543.570 kb on + strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.571 kb on - strand, within GFF499at 543.836 kb on + strand, within GFF500at 543.836 kb on + strand, within GFF500at 543.836 kb on + strand, within GFF500at 543.836 kb on + strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 543.837 kb on - strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.091 kb on + strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.092 kb on - strand, within GFF500at 544.100 kb on + strand, within GFF500at 544.101 kb on - strand, within GFF500at 544.101 kb on - strand, within GFF500at 544.101 kb on - strand, within GFF500at 544.397 kb on + strandat 544.397 kb on + strandat 544.397 kb on + strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.398 kb on - strandat 544.399 kb on + strandat 544.400 kb on - strandat 544.437 kb on - strandat 544.477 kb on - strandat 544.477 kb on - strandat 544.477 kb on - strandat 544.477 kb on - strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.485 kb on + strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.486 kb on - strandat 544.500 kb on + strand, within GFF501at 544.500 kb on + strand, within GFF501at 544.501 kb on - strand, within GFF501at 544.501 kb on - strand, within GFF501at 544.578 kb on + strand, within GFF501at 544.578 kb on + strand, within GFF501at 544.578 kb on + strand, within GFF501at 544.579 kb on - strand, within GFF501

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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542,980 + GFF498 0.68 +0.7
542,980 + GFF498 0.68 -0.4
542,980 + GFF498 0.68 -2.3
542,980 + GFF498 0.68 -0.4
542,980 + GFF498 0.68 +0.9
542,981 - GFF498 0.68 +0.5
542,981 - GFF498 0.68 -0.6
542,981 - GFF498 0.68 -0.3
542,981 - GFF498 0.68 +0.3
543,095 + GFF498 0.77 -2.7
543,298 + +0.1
543,393 + -0.8
543,394 - -0.2
543,394 - +0.5
543,409 - GFF499 0.11 -0.1
543,409 - GFF499 0.11 +1.7
543,481 + GFF499 0.34 +1.6
543,482 - GFF499 0.35 +0.8
543,482 - GFF499 0.35 -0.4
543,482 - GFF499 0.35 -0.2
543,482 - GFF499 0.35 -0.0
543,570 + GFF499 0.63 +2.2
543,570 + GFF499 0.63 -0.5
543,570 + GFF499 0.63 +0.7
543,571 - GFF499 0.63 -0.4
543,571 - GFF499 0.63 -0.1
543,571 - GFF499 0.63 -0.4
543,571 - GFF499 0.63 +1.1
543,836 + GFF500 0.21 -0.6
543,836 + GFF500 0.21 +0.2
543,836 + GFF500 0.21 -0.1
543,836 + GFF500 0.21 -1.3
543,837 - GFF500 0.21 -1.6
543,837 - GFF500 0.21 -0.2
543,837 - GFF500 0.21 -0.3
543,837 - GFF500 0.21 +0.8
543,837 - GFF500 0.21 +0.1
544,091 + GFF500 0.61 +0.0
544,091 + GFF500 0.61 -0.1
544,091 + GFF500 0.61 -0.8
544,091 + GFF500 0.61 +2.0
544,092 - GFF500 0.61 +0.4
544,092 - GFF500 0.61 -0.2
544,092 - GFF500 0.61 -1.0
544,092 - GFF500 0.61 -1.8
544,092 - GFF500 0.61 -0.4
544,100 + GFF500 0.62 +0.2
544,101 - GFF500 0.62 -1.6
544,101 - GFF500 0.62 -0.8
544,101 - GFF500 0.62 +0.1
544,397 + +0.9
544,397 + -0.3
544,397 + -0.3
544,398 - -0.2
544,398 - -0.3
544,398 - -0.5
544,398 - -0.1
544,398 - -0.8
544,398 - +0.8
544,398 - +0.2
544,399 + -0.8
544,400 - -0.8
544,437 - -0.1
544,477 - -0.4
544,477 - -0.2
544,477 - -0.1
544,477 - -0.6
544,485 + -0.3
544,485 + +1.1
544,485 + -0.6
544,485 + -0.6
544,485 + +0.3
544,485 + +0.4
544,486 - +0.8
544,486 - -1.2
544,486 - -0.6
544,486 - +0.7
544,486 - -0.1
544,486 - +0.7
544,500 + GFF501 0.12 +0.8
544,500 + GFF501 0.12 -0.6
544,501 - GFF501 0.12 -0.1
544,501 - GFF501 0.12 -0.6
544,578 + GFF501 0.37 -0.3
544,578 + GFF501 0.37 +2.2
544,578 + GFF501 0.37 -0.7
544,579 - GFF501 0.38 +0.1

Or see this region's nucleotide sequence