Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4905 and GFF4906 are separated by 105 nucleotides
GFF4905 - hypothetical protein, at 269,740 to 271,149
GFF4905
GFF4906 - hypothetical protein, at 271,255 to 273,033
GFF4906
Position (kb)
271
272
273
274 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 270.269 kb on - strand, within GFF4905 at 270.269 kb on - strand, within GFF4905 at 270.301 kb on + strand, within GFF4905 at 270.301 kb on + strand, within GFF4905 at 270.301 kb on + strand, within GFF4905 at 270.301 kb on + strand, within GFF4905 at 270.301 kb on + strand, within GFF4905 at 270.301 kb on + strand, within GFF4905 at 270.502 kb on + strand, within GFF4905 at 270.502 kb on + strand, within GFF4905 at 270.502 kb on + strand, within GFF4905 at 270.503 kb on - strand, within GFF4905 at 270.503 kb on - strand, within GFF4905 at 270.503 kb on - strand, within GFF4905 at 270.503 kb on - strand, within GFF4905 at 270.637 kb on + strand, within GFF4905 at 270.637 kb on + strand, within GFF4905 at 270.637 kb on + strand, within GFF4905 at 270.637 kb on + strand, within GFF4905 at 270.638 kb on - strand, within GFF4905 at 270.638 kb on - strand, within GFF4905 at 270.638 kb on - strand, within GFF4905 at 270.638 kb on - strand, within GFF4905 at 270.638 kb on - strand, within GFF4905 at 270.638 kb on - strand, within GFF4905 at 271.147 kb on + strand at 271.147 kb on + strand at 271.147 kb on + strand at 271.148 kb on - strand at 271.174 kb on + strand at 271.175 kb on - strand at 271.175 kb on - strand at 271.175 kb on - strand at 271.175 kb on - strand at 271.175 kb on - strand at 271.794 kb on + strand, within GFF4906 at 271.795 kb on - strand, within GFF4906 at 271.802 kb on - strand, within GFF4906 at 271.802 kb on - strand, within GFF4906 at 271.802 kb on - strand, within GFF4906 at 271.802 kb on - strand, within GFF4906 at 271.837 kb on + strand, within GFF4906 at 271.838 kb on - strand, within GFF4906 at 271.838 kb on - strand, within GFF4906 at 271.838 kb on - strand, within GFF4906 at 271.838 kb on - strand, within GFF4906 at 272.103 kb on + strand, within GFF4906 at 272.103 kb on + strand, within GFF4906 at 272.103 kb on + strand, within GFF4906 at 272.124 kb on + strand, within GFF4906 at 272.124 kb on + strand, within GFF4906 at 272.125 kb on - strand, within GFF4906 at 272.639 kb on - strand, within GFF4906 at 272.639 kb on - strand, within GFF4906 at 272.639 kb on - strand, within GFF4906 at 272.639 kb on - strand, within GFF4906 at 272.761 kb on + strand, within GFF4906 at 272.761 kb on + strand, within GFF4906 at 272.761 kb on + strand, within GFF4906 at 272.905 kb on + strand at 272.905 kb on + strand at 272.906 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 270,269 - GFF4905 0.38 -0.6 270,269 - GFF4905 0.38 -0.5 270,301 + GFF4905 0.40 -1.2 270,301 + GFF4905 0.40 -0.4 270,301 + GFF4905 0.40 +0.1 270,301 + GFF4905 0.40 -0.3 270,301 + GFF4905 0.40 -0.7 270,301 + GFF4905 0.40 +0.6 270,502 + GFF4905 0.54 +0.5 270,502 + GFF4905 0.54 -0.6 270,502 + GFF4905 0.54 +0.3 270,503 - GFF4905 0.54 +0.1 270,503 - GFF4905 0.54 +0.4 270,503 - GFF4905 0.54 -0.8 270,503 - GFF4905 0.54 -0.6 270,637 + GFF4905 0.64 +0.4 270,637 + GFF4905 0.64 +0.0 270,637 + GFF4905 0.64 -0.1 270,637 + GFF4905 0.64 -0.5 270,638 - GFF4905 0.64 -0.4 270,638 - GFF4905 0.64 -1.2 270,638 - GFF4905 0.64 -0.3 270,638 - GFF4905 0.64 -0.1 270,638 - GFF4905 0.64 -0.2 270,638 - GFF4905 0.64 +1.4 271,147 + -0.3 271,147 + +0.7 271,147 + -0.3 271,148 - +0.2 271,174 + +0.9 271,175 - -1.0 271,175 - -0.4 271,175 - -0.7 271,175 - -0.3 271,175 - -0.3 271,794 + GFF4906 0.30 -0.0 271,795 - GFF4906 0.30 -1.8 271,802 - GFF4906 0.31 +1.4 271,802 - GFF4906 0.31 -0.6 271,802 - GFF4906 0.31 +1.7 271,802 - GFF4906 0.31 -0.3 271,837 + GFF4906 0.33 +1.4 271,838 - GFF4906 0.33 -0.1 271,838 - GFF4906 0.33 -1.6 271,838 - GFF4906 0.33 -0.4 271,838 - GFF4906 0.33 +0.3 272,103 + GFF4906 0.48 -1.7 272,103 + GFF4906 0.48 +0.6 272,103 + GFF4906 0.48 +0.6 272,124 + GFF4906 0.49 +0.9 272,124 + GFF4906 0.49 +0.5 272,125 - GFF4906 0.49 +0.1 272,639 - GFF4906 0.78 -0.5 272,639 - GFF4906 0.78 -0.5 272,639 - GFF4906 0.78 +2.7 272,639 - GFF4906 0.78 -0.5 272,761 + GFF4906 0.85 +0.3 272,761 + GFF4906 0.85 -1.6 272,761 + GFF4906 0.85 +0.4 272,905 + -0.7 272,905 + -1.1 272,906 - -0.3
Or see this region's nucleotide sequence