Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4581 and GFF4582 are separated by 352 nucleotides GFF4582 and GFF4583 overlap by 4 nucleotides GFF4583 and GFF4584 are separated by 94 nucleotides
GFF4581 - hypothetical protein, at 222,853 to 223,146
GFF4581
GFF4582 - TRAP-type C4-dicarboxylate transport system, small permease component, at 223,499 to 224,098
GFF4582
GFF4583 - TRAP-type C4-dicarboxylate transport system, large permease component, at 224,095 to 225,393
GFF4583
GFF4584 - TRAP-type transport system, periplasmic component, predicted N-acetylneuraminate-binding protein, at 225,488 to 226,525
GFF4584
Position (kb) 224
225
226 Strain fitness (log2 ratio) -2
-1
0
1
2 at 223.127 kb on - strand at 223.135 kb on + strand at 223.135 kb on + strand at 223.135 kb on + strand at 223.368 kb on + strand at 223.384 kb on - strand at 223.406 kb on + strand at 223.406 kb on + strand at 223.406 kb on + strand at 223.406 kb on + strand at 223.406 kb on + strand at 223.406 kb on + strand at 223.406 kb on + strand at 223.406 kb on + strand at 223.407 kb on - strand at 223.407 kb on - strand at 223.407 kb on - strand at 223.407 kb on - strand at 223.407 kb on - strand at 223.437 kb on + strand at 223.489 kb on - strand at 223.593 kb on + strand, within GFF4582 at 223.594 kb on - strand, within GFF4582 at 223.594 kb on - strand, within GFF4582 at 223.670 kb on + strand, within GFF4582 at 223.787 kb on + strand, within GFF4582 at 223.787 kb on + strand, within GFF4582 at 223.955 kb on + strand, within GFF4582 at 224.004 kb on + strand, within GFF4582 at 224.005 kb on - strand, within GFF4582 at 224.005 kb on - strand, within GFF4582 at 224.040 kb on - strand at 224.267 kb on - strand, within GFF4583 at 224.852 kb on - strand, within GFF4583 at 224.852 kb on - strand, within GFF4583 at 224.852 kb on - strand, within GFF4583 at 224.852 kb on - strand, within GFF4583 at 224.981 kb on - strand, within GFF4583 at 225.049 kb on + strand, within GFF4583 at 225.157 kb on + strand, within GFF4583 at 225.157 kb on + strand, within GFF4583 at 225.157 kb on + strand, within GFF4583 at 225.158 kb on - strand, within GFF4583 at 225.160 kb on + strand, within GFF4583 at 225.160 kb on + strand, within GFF4583 at 225.160 kb on + strand, within GFF4583 at 225.160 kb on + strand, within GFF4583 at 225.161 kb on - strand, within GFF4583 at 225.161 kb on - strand, within GFF4583 at 225.161 kb on - strand, within GFF4583 at 225.161 kb on - strand, within GFF4583 at 225.161 kb on - strand, within GFF4583 at 225.161 kb on - strand, within GFF4583 at 225.388 kb on + strand at 225.458 kb on + strand at 225.459 kb on - strand at 225.474 kb on + strand at 225.474 kb on + strand at 225.587 kb on + strand at 225.587 kb on + strand at 225.588 kb on - strand at 225.588 kb on - strand at 225.849 kb on - strand, within GFF4584 at 226.205 kb on + strand, within GFF4584 at 226.206 kb on - strand, within GFF4584
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 223,127 - +0.1 223,135 + -1.4 223,135 + +0.7 223,135 + -0.4 223,368 + +0.0 223,384 - -0.1 223,406 + -0.0 223,406 + +0.0 223,406 + -0.2 223,406 + -0.9 223,406 + +0.7 223,406 + -0.7 223,406 + -0.3 223,406 + +1.2 223,407 - -1.1 223,407 - -1.1 223,407 - -0.2 223,407 - +0.1 223,407 - +0.1 223,437 + -1.7 223,489 - +0.2 223,593 + GFF4582 0.16 -1.2 223,594 - GFF4582 0.16 +0.5 223,594 - GFF4582 0.16 -0.9 223,670 + GFF4582 0.28 -0.6 223,787 + GFF4582 0.48 +1.4 223,787 + GFF4582 0.48 +0.9 223,955 + GFF4582 0.76 +0.1 224,004 + GFF4582 0.84 -0.5 224,005 - GFF4582 0.84 -0.4 224,005 - GFF4582 0.84 +1.7 224,040 - -0.2 224,267 - GFF4583 0.13 -0.5 224,852 - GFF4583 0.58 -0.6 224,852 - GFF4583 0.58 -1.7 224,852 - GFF4583 0.58 +1.1 224,852 - GFF4583 0.58 -0.6 224,981 - GFF4583 0.68 +0.1 225,049 + GFF4583 0.73 -0.4 225,157 + GFF4583 0.82 +1.6 225,157 + GFF4583 0.82 +0.1 225,157 + GFF4583 0.82 +0.7 225,158 - GFF4583 0.82 +1.0 225,160 + GFF4583 0.82 -1.6 225,160 + GFF4583 0.82 -1.0 225,160 + GFF4583 0.82 -0.7 225,160 + GFF4583 0.82 -1.7 225,161 - GFF4583 0.82 -1.1 225,161 - GFF4583 0.82 -0.6 225,161 - GFF4583 0.82 -0.1 225,161 - GFF4583 0.82 -0.5 225,161 - GFF4583 0.82 -1.1 225,161 - GFF4583 0.82 -1.6 225,388 + +2.1 225,458 + +0.8 225,459 - -1.4 225,474 + -0.3 225,474 + -0.5 225,587 + +0.1 225,587 + -0.9 225,588 - -0.6 225,588 - -1.1 225,849 - GFF4584 0.35 +0.8 226,205 + GFF4584 0.69 -0.3 226,206 - GFF4584 0.69 -2.1
Or see this region's nucleotide sequence