Strain Fitness in Variovorax sp. SCN45 around GFF457

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF456 and GFF457 are separated by 188 nucleotidesGFF457 and GFF458 are separated by 25 nucleotides GFF456 - Transcriptional regulator, AraC family, at 493,861 to 494,823 GFF456 GFF457 - Nitrilase (EC 3.5.5.7), at 495,012 to 496,016 GFF457 GFF458 - Aldoxime dehydratase, at 496,042 to 497,097 GFF458 Position (kb) 495 496 497Strain fitness (log2 ratio) -2 -1 0 1 2 3at 494.042 kb on + strand, within GFF456at 494.042 kb on + strand, within GFF456at 494.043 kb on - strand, within GFF456at 494.043 kb on - strand, within GFF456at 494.043 kb on - strand, within GFF456at 494.043 kb on - strand, within GFF456at 494.043 kb on - strand, within GFF456at 494.043 kb on - strand, within GFF456at 494.187 kb on - strand, within GFF456at 494.187 kb on - strand, within GFF456at 494.484 kb on + strand, within GFF456at 494.484 kb on + strand, within GFF456at 494.492 kb on + strand, within GFF456at 494.492 kb on + strand, within GFF456at 494.492 kb on + strand, within GFF456at 494.493 kb on - strand, within GFF456at 494.493 kb on - strand, within GFF456at 494.582 kb on + strand, within GFF456at 494.583 kb on - strand, within GFF456at 494.744 kb on + strandat 494.744 kb on + strandat 494.744 kb on + strandat 494.745 kb on - strandat 494.745 kb on - strandat 494.771 kb on + strandat 494.772 kb on - strandat 494.925 kb on + strandat 494.925 kb on + strandat 494.943 kb on + strandat 494.944 kb on - strandat 494.986 kb on - strandat 495.174 kb on + strand, within GFF457at 495.348 kb on + strand, within GFF457at 495.348 kb on + strand, within GFF457at 495.348 kb on + strand, within GFF457at 495.349 kb on - strand, within GFF457at 495.534 kb on + strand, within GFF457at 495.534 kb on + strand, within GFF457at 495.535 kb on - strand, within GFF457at 495.535 kb on - strand, within GFF457at 495.535 kb on - strand, within GFF457at 495.535 kb on - strand, within GFF457at 495.543 kb on + strand, within GFF457at 495.543 kb on + strand, within GFF457at 495.544 kb on - strand, within GFF457at 495.595 kb on - strand, within GFF457at 495.609 kb on + strand, within GFF457at 495.609 kb on + strand, within GFF457at 495.610 kb on - strand, within GFF457at 495.610 kb on - strand, within GFF457at 495.610 kb on - strand, within GFF457at 495.610 kb on - strand, within GFF457at 495.651 kb on + strand, within GFF457at 495.651 kb on + strand, within GFF457at 495.651 kb on + strand, within GFF457at 495.651 kb on + strand, within GFF457at 495.651 kb on + strand, within GFF457at 495.651 kb on + strand, within GFF457at 495.651 kb on + strand, within GFF457at 495.652 kb on - strand, within GFF457at 495.652 kb on - strand, within GFF457at 495.652 kb on - strand, within GFF457at 495.652 kb on - strand, within GFF457at 495.652 kb on - strand, within GFF457at 495.679 kb on + strand, within GFF457at 495.780 kb on + strand, within GFF457at 495.780 kb on + strand, within GFF457at 495.781 kb on - strand, within GFF457at 495.781 kb on - strand, within GFF457at 495.781 kb on - strand, within GFF457at 495.781 kb on - strand, within GFF457at 495.903 kb on + strand, within GFF457at 495.904 kb on - strand, within GFF457at 495.904 kb on - strand, within GFF457at 496.108 kb on + strandat 496.109 kb on - strandat 496.109 kb on - strandat 496.291 kb on + strand, within GFF458at 496.291 kb on + strand, within GFF458at 496.292 kb on - strand, within GFF458at 496.333 kb on + strand, within GFF458at 496.412 kb on + strand, within GFF458at 496.412 kb on + strand, within GFF458at 496.413 kb on - strand, within GFF458at 496.552 kb on + strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.742 kb on - strand, within GFF458at 496.817 kb on - strand, within GFF458at 496.828 kb on + strand, within GFF458at 496.966 kb on + strand, within GFF458at 496.966 kb on + strand, within GFF458at 497.006 kb on - strandat 497.006 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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494,042 + GFF456 0.19 +0.8
494,042 + GFF456 0.19 -0.3
494,043 - GFF456 0.19 +0.2
494,043 - GFF456 0.19 -0.0
494,043 - GFF456 0.19 +0.6
494,043 - GFF456 0.19 -0.6
494,043 - GFF456 0.19 -0.8
494,043 - GFF456 0.19 +2.8
494,187 - GFF456 0.34 -1.1
494,187 - GFF456 0.34 +0.2
494,484 + GFF456 0.65 -0.3
494,484 + GFF456 0.65 +1.2
494,492 + GFF456 0.66 +0.1
494,492 + GFF456 0.66 +0.7
494,492 + GFF456 0.66 +0.4
494,493 - GFF456 0.66 -0.8
494,493 - GFF456 0.66 +0.1
494,582 + GFF456 0.75 -1.5
494,583 - GFF456 0.75 -0.6
494,744 + +0.2
494,744 + -0.8
494,744 + +0.7
494,745 - +0.1
494,745 - +0.2
494,771 + -0.5
494,772 - -0.1
494,925 + +1.2
494,925 + -0.3
494,943 + +0.0
494,944 - +0.1
494,986 - -1.2
495,174 + GFF457 0.16 +0.7
495,348 + GFF457 0.33 -1.4
495,348 + GFF457 0.33 -0.8
495,348 + GFF457 0.33 -0.5
495,349 - GFF457 0.34 +0.0
495,534 + GFF457 0.52 -0.4
495,534 + GFF457 0.52 -0.7
495,535 - GFF457 0.52 -0.3
495,535 - GFF457 0.52 -0.6
495,535 - GFF457 0.52 -0.2
495,535 - GFF457 0.52 +0.5
495,543 + GFF457 0.53 -0.1
495,543 + GFF457 0.53 +0.3
495,544 - GFF457 0.53 +0.9
495,595 - GFF457 0.58 -0.0
495,609 + GFF457 0.59 +0.7
495,609 + GFF457 0.59 +0.4
495,610 - GFF457 0.60 +0.1
495,610 - GFF457 0.60 +0.1
495,610 - GFF457 0.60 -0.7
495,610 - GFF457 0.60 -1.0
495,651 + GFF457 0.64 +0.4
495,651 + GFF457 0.64 -1.8
495,651 + GFF457 0.64 +1.2
495,651 + GFF457 0.64 -0.2
495,651 + GFF457 0.64 -0.2
495,651 + GFF457 0.64 +1.8
495,651 + GFF457 0.64 +0.1
495,652 - GFF457 0.64 -0.9
495,652 - GFF457 0.64 -2.5
495,652 - GFF457 0.64 +0.2
495,652 - GFF457 0.64 -1.0
495,652 - GFF457 0.64 -0.0
495,679 + GFF457 0.66 -1.3
495,780 + GFF457 0.76 -0.4
495,780 + GFF457 0.76 +1.2
495,781 - GFF457 0.77 +1.2
495,781 - GFF457 0.77 +0.5
495,781 - GFF457 0.77 -0.2
495,781 - GFF457 0.77 -0.1
495,903 + GFF457 0.89 -0.6
495,904 - GFF457 0.89 +0.7
495,904 - GFF457 0.89 +0.2
496,108 + -0.4
496,109 - +0.7
496,109 - -0.9
496,291 + GFF458 0.24 +0.6
496,291 + GFF458 0.24 -1.2
496,292 - GFF458 0.24 +0.3
496,333 + GFF458 0.28 -0.5
496,412 + GFF458 0.35 -0.4
496,412 + GFF458 0.35 +0.2
496,413 - GFF458 0.35 -1.3
496,552 + GFF458 0.48 -0.1
496,742 - GFF458 0.66 +0.1
496,742 - GFF458 0.66 -0.7
496,742 - GFF458 0.66 -1.0
496,742 - GFF458 0.66 +1.2
496,817 - GFF458 0.73 -0.5
496,828 + GFF458 0.74 -1.1
496,966 + GFF458 0.88 +0.7
496,966 + GFF458 0.88 -0.9
497,006 - +0.2
497,006 - +1.6

Or see this region's nucleotide sequence