Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF452 and GFF453 are separated by 97 nucleotides GFF453 and GFF454 overlap by 4 nucleotides GFF454 and GFF455 are separated by 303 nucleotides GFF455 and GFF456 are separated by 22 nucleotides
GFF452 - Chloride channel protein, at 490,329 to 492,044
GFF452
GFF453 - hypothetical protein, at 492,142 to 492,366
GFF453
GFF454 - Z-ring-associated protein ZapA, at 492,363 to 492,707
GFF454
GFF455 - Uncharacterized UPF0721 integral membrane protein, at 493,011 to 493,838
GFF455
GFF456 - Transcriptional regulator, AraC family, at 493,861 to 494,823
GFF456
Position (kb) 493
494 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.024 kb on + strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.025 kb on - strand at 492.043 kb on - strand at 492.043 kb on - strand at 492.043 kb on - strand at 492.043 kb on - strand at 492.088 kb on - strand at 492.128 kb on + strand at 492.128 kb on + strand at 492.128 kb on + strand at 492.128 kb on + strand at 492.129 kb on - strand at 492.129 kb on - strand at 492.129 kb on - strand at 492.379 kb on + strand at 492.379 kb on + strand at 492.379 kb on + strand at 492.380 kb on - strand at 492.380 kb on - strand at 492.399 kb on + strand, within GFF454 at 492.399 kb on + strand, within GFF454 at 492.399 kb on + strand, within GFF454 at 492.399 kb on + strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.400 kb on - strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.592 kb on + strand, within GFF454 at 492.593 kb on - strand, within GFF454 at 492.626 kb on + strand, within GFF454 at 492.626 kb on + strand, within GFF454 at 492.626 kb on + strand, within GFF454 at 492.627 kb on - strand, within GFF454 at 492.738 kb on + strand at 492.774 kb on + strand at 492.774 kb on + strand at 492.775 kb on - strand at 492.775 kb on - strand at 492.775 kb on - strand at 492.812 kb on + strand at 492.812 kb on + strand at 492.812 kb on + strand at 492.812 kb on + strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.813 kb on - strand at 492.820 kb on + strand at 492.821 kb on - strand at 492.911 kb on + strand at 493.002 kb on + strand at 493.003 kb on - strand at 493.620 kb on + strand, within GFF455 at 493.620 kb on + strand, within GFF455 at 493.620 kb on + strand, within GFF455 at 493.620 kb on + strand, within GFF455 at 493.621 kb on - strand, within GFF455 at 493.621 kb on - strand, within GFF455 at 493.621 kb on - strand, within GFF455 at 493.671 kb on + strand, within GFF455 at 493.671 kb on + strand, within GFF455 at 493.671 kb on + strand, within GFF455 at 493.672 kb on - strand, within GFF455 at 493.672 kb on - strand, within GFF455 at 493.672 kb on - strand, within GFF455 at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.794 kb on + strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.795 kb on - strand at 493.809 kb on + strand at 493.809 kb on + strand at 493.809 kb on + strand at 493.809 kb on + strand at 493.810 kb on - strand at 493.810 kb on - strand at 493.810 kb on - strand at 493.810 kb on - strand at 493.810 kb on - strand at 493.810 kb on - strand at 493.810 kb on - strand at 493.958 kb on + strand, within GFF456 at 493.958 kb on + strand, within GFF456 at 493.958 kb on + strand, within GFF456 at 493.959 kb on - strand, within GFF456 at 493.959 kb on - strand, within GFF456 at 494.042 kb on + strand, within GFF456 at 494.042 kb on + strand, within GFF456 at 494.043 kb on - strand, within GFF456 at 494.043 kb on - strand, within GFF456 at 494.043 kb on - strand, within GFF456 at 494.043 kb on - strand, within GFF456 at 494.043 kb on - strand, within GFF456 at 494.043 kb on - strand, within GFF456 at 494.187 kb on - strand, within GFF456 at 494.187 kb on - strand, within GFF456 at 494.484 kb on + strand, within GFF456 at 494.484 kb on + strand, within GFF456 at 494.492 kb on + strand, within GFF456 at 494.492 kb on + strand, within GFF456 at 494.492 kb on + strand, within GFF456 at 494.493 kb on - strand, within GFF456 at 494.493 kb on - strand, within GFF456 at 494.582 kb on + strand, within GFF456 at 494.583 kb on - strand, within GFF456 at 494.744 kb on + strand at 494.744 kb on + strand at 494.744 kb on + strand at 494.745 kb on - strand at 494.745 kb on - strand at 494.771 kb on + strand at 494.772 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 492,024 + -0.3 492,024 + +1.2 492,024 + -0.6 492,024 + +0.9 492,024 + -0.8 492,024 + +0.1 492,024 + -0.6 492,024 + +0.1 492,024 + +1.0 492,025 - -1.2 492,025 - -0.2 492,025 - -0.5 492,025 - +1.2 492,025 - -0.8 492,025 - -0.2 492,025 - +0.2 492,025 - +1.5 492,025 - -0.2 492,043 - -0.3 492,043 - +0.4 492,043 - -0.8 492,043 - -1.9 492,088 - -0.2 492,128 + +0.7 492,128 + -0.2 492,128 + -0.6 492,128 + -0.2 492,129 - +1.6 492,129 - +0.3 492,129 - -0.1 492,379 + -0.8 492,379 + -1.6 492,379 + -0.7 492,380 - -0.9 492,380 - -0.5 492,399 + GFF454 0.10 -0.3 492,399 + GFF454 0.10 +0.0 492,399 + GFF454 0.10 -0.6 492,399 + GFF454 0.10 -0.9 492,400 - GFF454 0.11 +0.6 492,400 - GFF454 0.11 -0.6 492,400 - GFF454 0.11 -1.3 492,400 - GFF454 0.11 -0.4 492,592 + GFF454 0.66 -0.4 492,592 + GFF454 0.66 -0.9 492,592 + GFF454 0.66 +0.7 492,592 + GFF454 0.66 -0.2 492,592 + GFF454 0.66 +0.2 492,593 - GFF454 0.67 -0.2 492,626 + GFF454 0.76 -0.3 492,626 + GFF454 0.76 +0.2 492,626 + GFF454 0.76 +0.2 492,627 - GFF454 0.77 -0.2 492,738 + -0.3 492,774 + +1.7 492,774 + -0.5 492,775 - -0.8 492,775 - -0.3 492,775 - -2.8 492,812 + +0.1 492,812 + +0.0 492,812 + -0.2 492,812 + -1.2 492,813 - +0.7 492,813 - -0.4 492,813 - -1.6 492,813 - -1.2 492,813 - +0.4 492,813 - -0.9 492,813 - -1.8 492,820 + +1.2 492,821 - +1.1 492,911 + -0.2 493,002 + -0.4 493,003 - -0.5 493,620 + GFF455 0.74 +1.1 493,620 + GFF455 0.74 -0.0 493,620 + GFF455 0.74 +0.2 493,620 + GFF455 0.74 -1.3 493,621 - GFF455 0.74 -0.1 493,621 - GFF455 0.74 +0.9 493,621 - GFF455 0.74 +0.5 493,671 + GFF455 0.80 -1.0 493,671 + GFF455 0.80 -1.1 493,671 + GFF455 0.80 -0.5 493,672 - GFF455 0.80 +0.0 493,672 - GFF455 0.80 +0.5 493,672 - GFF455 0.80 -0.6 493,794 + +0.1 493,794 + -1.5 493,794 + -0.4 493,794 + -0.6 493,794 + -0.2 493,794 + +0.2 493,794 + -1.3 493,794 + -0.5 493,794 + -1.0 493,794 + +0.2 493,794 + +0.2 493,795 - +0.1 493,795 - -0.8 493,795 - -0.9 493,795 - -0.2 493,795 - -0.3 493,795 - +0.1 493,795 - -0.4 493,795 - -1.1 493,795 - -0.4 493,795 - -0.5 493,795 - -0.7 493,795 - +0.2 493,809 + -1.3 493,809 + -0.9 493,809 + -1.0 493,809 + +0.3 493,810 - -0.4 493,810 - +0.4 493,810 - +1.8 493,810 - -0.4 493,810 - -0.2 493,810 - -0.3 493,810 - +0.9 493,958 + GFF456 0.10 -0.2 493,958 + GFF456 0.10 +2.8 493,958 + GFF456 0.10 -0.3 493,959 - GFF456 0.10 -0.9 493,959 - GFF456 0.10 +0.2 494,042 + GFF456 0.19 +0.8 494,042 + GFF456 0.19 -0.3 494,043 - GFF456 0.19 +0.2 494,043 - GFF456 0.19 -0.0 494,043 - GFF456 0.19 +0.6 494,043 - GFF456 0.19 -0.6 494,043 - GFF456 0.19 -0.8 494,043 - GFF456 0.19 +2.8 494,187 - GFF456 0.34 -1.1 494,187 - GFF456 0.34 +0.2 494,484 + GFF456 0.65 -0.3 494,484 + GFF456 0.65 +1.2 494,492 + GFF456 0.66 +0.1 494,492 + GFF456 0.66 +0.7 494,492 + GFF456 0.66 +0.4 494,493 - GFF456 0.66 -0.8 494,493 - GFF456 0.66 +0.1 494,582 + GFF456 0.75 -1.5 494,583 - GFF456 0.75 -0.6 494,744 + +0.2 494,744 + -0.8 494,744 + +0.7 494,745 - +0.1 494,745 - +0.2 494,771 + -0.5 494,772 - -0.1
Or see this region's nucleotide sequence