Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4540 and GFF4541 are separated by 4 nucleotides GFF4541 and GFF4542 are separated by 97 nucleotides GFF4542 and GFF4543 overlap by 4 nucleotides
GFF4540 - hypothetical protein, at 178,207 to 179,388
GFF4540
GFF4541 - Uncharacterized protein conserved in bacteria, at 179,393 to 180,772
GFF4541
GFF4542 - hypothetical protein, at 180,870 to 181,364
GFF4542
GFF4543 - no description, at 181,361 to 181,795
GFF4543
Position (kb) 179
180
181 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 178.504 kb on + strand, within GFF4540 at 178.799 kb on + strand, within GFF4540 at 178.799 kb on + strand, within GFF4540 at 178.799 kb on + strand, within GFF4540 at 178.799 kb on + strand, within GFF4540 at 178.799 kb on + strand, within GFF4540 at 178.799 kb on + strand, within GFF4540 at 178.800 kb on - strand, within GFF4540 at 178.800 kb on - strand, within GFF4540 at 178.800 kb on - strand, within GFF4540 at 178.800 kb on - strand, within GFF4540 at 178.860 kb on - strand, within GFF4540 at 179.042 kb on + strand, within GFF4540 at 179.087 kb on + strand, within GFF4540 at 179.088 kb on - strand, within GFF4540 at 179.190 kb on - strand, within GFF4540 at 179.204 kb on + strand, within GFF4540 at 179.368 kb on + strand at 179.368 kb on + strand at 179.368 kb on + strand at 179.368 kb on + strand at 179.369 kb on - strand at 179.369 kb on - strand at 179.369 kb on - strand at 179.369 kb on - strand at 179.802 kb on + strand, within GFF4541 at 179.802 kb on + strand, within GFF4541 at 179.985 kb on + strand, within GFF4541 at 179.986 kb on - strand, within GFF4541 at 179.986 kb on - strand, within GFF4541 at 180.144 kb on + strand, within GFF4541 at 180.144 kb on + strand, within GFF4541 at 180.145 kb on - strand, within GFF4541 at 180.145 kb on - strand, within GFF4541 at 180.360 kb on + strand, within GFF4541 at 180.360 kb on + strand, within GFF4541 at 180.361 kb on - strand, within GFF4541 at 180.361 kb on - strand, within GFF4541 at 180.498 kb on + strand, within GFF4541 at 180.498 kb on + strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.871 kb on + strand at 180.871 kb on + strand at 180.872 kb on - strand at 180.872 kb on - strand at 181.384 kb on - strand at 181.758 kb on + strand at 181.758 kb on + strand at 181.759 kb on - strand at 181.759 kb on - strand at 181.759 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 178,504 + GFF4540 0.25 -0.3 178,799 + GFF4540 0.50 -0.4 178,799 + GFF4540 0.50 +0.4 178,799 + GFF4540 0.50 -1.4 178,799 + GFF4540 0.50 -0.4 178,799 + GFF4540 0.50 -1.0 178,799 + GFF4540 0.50 -0.4 178,800 - GFF4540 0.50 +1.2 178,800 - GFF4540 0.50 +0.7 178,800 - GFF4540 0.50 -1.0 178,800 - GFF4540 0.50 +0.2 178,860 - GFF4540 0.55 -1.6 179,042 + GFF4540 0.71 +0.1 179,087 + GFF4540 0.74 -0.6 179,088 - GFF4540 0.75 +0.1 179,190 - GFF4540 0.83 +0.9 179,204 + GFF4540 0.84 +1.3 179,368 + +0.5 179,368 + -0.9 179,368 + +0.5 179,368 + +1.3 179,369 - +0.3 179,369 - -0.3 179,369 - -0.6 179,369 - -0.7 179,802 + GFF4541 0.30 -0.3 179,802 + GFF4541 0.30 -1.7 179,985 + GFF4541 0.43 -0.7 179,986 - GFF4541 0.43 +0.0 179,986 - GFF4541 0.43 -1.9 180,144 + GFF4541 0.54 -0.1 180,144 + GFF4541 0.54 -0.5 180,145 - GFF4541 0.54 -0.7 180,145 - GFF4541 0.54 +0.1 180,360 + GFF4541 0.70 -0.3 180,360 + GFF4541 0.70 +0.3 180,361 - GFF4541 0.70 -0.9 180,361 - GFF4541 0.70 -1.1 180,498 + GFF4541 0.80 -1.2 180,498 + GFF4541 0.80 -0.2 180,499 - GFF4541 0.80 +0.0 180,499 - GFF4541 0.80 -0.2 180,499 - GFF4541 0.80 -0.3 180,499 - GFF4541 0.80 +0.5 180,588 + GFF4541 0.87 -1.4 180,588 + GFF4541 0.87 +1.0 180,588 + GFF4541 0.87 +1.3 180,588 + GFF4541 0.87 +0.4 180,588 + GFF4541 0.87 -0.1 180,589 - GFF4541 0.87 +0.7 180,589 - GFF4541 0.87 -0.6 180,589 - GFF4541 0.87 -1.0 180,589 - GFF4541 0.87 -0.1 180,589 - GFF4541 0.87 +0.6 180,589 - GFF4541 0.87 -1.3 180,589 - GFF4541 0.87 -0.4 180,589 - GFF4541 0.87 -1.2 180,589 - GFF4541 0.87 -0.6 180,589 - GFF4541 0.87 -3.0 180,589 - GFF4541 0.87 -0.2 180,589 - GFF4541 0.87 +1.9 180,871 + -0.4 180,871 + +0.6 180,872 - -0.4 180,872 - +0.5 181,384 - +0.3 181,758 + -1.7 181,758 + -0.7 181,759 - -0.7 181,759 - -0.4 181,759 - -1.3
Or see this region's nucleotide sequence