Strain Fitness in Variovorax sp. SCN45 around GFF4524

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4523 and GFF4524 are separated by 23 nucleotidesGFF4524 and GFF4526 overlap by 4 nucleotidesGFF4526 and GFF4525 overlap by 2 nucleotidesGFF4525 and GFF4527 are separated by 33 nucleotides GFF4523 - Cytosine deaminase (EC 3.5.4.1), at 162,194 to 163,408 GFF4523 GFF4524 - Uncharacterized symporter YhjB, at 163,432 to 164,907 GFF4524 GFF4526 - no description, at 164,904 to 165,119 GFF4526 GFF4525 - no description, at 165,118 to 165,261 GFF4525 GFF4527 - Transcriptional regulator, GntR family, at 165,295 to 166,077 GFF4527 Position (kb) 163 164 165Strain fitness (log2 ratio) -2 -1 0 1at 162.435 kb on + strand, within GFF4523at 162.435 kb on + strand, within GFF4523at 162.435 kb on + strand, within GFF4523at 162.436 kb on - strand, within GFF4523at 162.436 kb on - strand, within GFF4523at 162.436 kb on - strand, within GFF4523at 162.503 kb on + strand, within GFF4523at 162.503 kb on + strand, within GFF4523at 162.503 kb on + strand, within GFF4523at 162.662 kb on + strand, within GFF4523at 162.662 kb on + strand, within GFF4523at 162.663 kb on - strand, within GFF4523at 162.663 kb on - strand, within GFF4523at 162.812 kb on + strand, within GFF4523at 162.813 kb on - strand, within GFF4523at 163.576 kb on - strandat 163.576 kb on - strandat 163.769 kb on + strand, within GFF4524at 163.770 kb on - strand, within GFF4524at 163.860 kb on - strand, within GFF4524at 163.860 kb on - strand, within GFF4524at 164.081 kb on + strand, within GFF4524at 164.082 kb on - strand, within GFF4524at 164.082 kb on - strand, within GFF4524at 164.136 kb on - strand, within GFF4524at 164.160 kb on - strand, within GFF4524at 164.165 kb on + strand, within GFF4524at 164.165 kb on + strand, within GFF4524at 164.166 kb on - strand, within GFF4524at 164.166 kb on - strand, within GFF4524at 164.166 kb on - strand, within GFF4524at 164.166 kb on - strand, within GFF4524at 164.166 kb on - strand, within GFF4524at 164.166 kb on - strand, within GFF4524at 164.168 kb on + strand, within GFF4524at 164.169 kb on - strand, within GFF4524at 164.202 kb on - strand, within GFF4524at 164.291 kb on + strand, within GFF4524at 164.291 kb on + strand, within GFF4524at 164.292 kb on - strand, within GFF4524at 164.294 kb on + strand, within GFF4524at 164.295 kb on - strand, within GFF4524at 164.309 kb on + strand, within GFF4524at 164.309 kb on + strand, within GFF4524at 164.309 kb on + strand, within GFF4524at 164.309 kb on + strand, within GFF4524at 164.399 kb on + strand, within GFF4524at 164.399 kb on + strand, within GFF4524at 164.399 kb on + strand, within GFF4524at 164.399 kb on + strand, within GFF4524at 164.399 kb on + strand, within GFF4524at 164.400 kb on - strand, within GFF4524at 164.400 kb on - strand, within GFF4524at 164.400 kb on - strand, within GFF4524at 164.400 kb on - strand, within GFF4524at 164.400 kb on - strand, within GFF4524at 164.400 kb on - strand, within GFF4524at 164.567 kb on + strand, within GFF4524at 164.567 kb on + strand, within GFF4524at 164.567 kb on + strand, within GFF4524at 164.567 kb on + strand, within GFF4524at 164.567 kb on + strand, within GFF4524at 164.568 kb on - strand, within GFF4524at 164.568 kb on - strand, within GFF4524at 164.568 kb on - strand, within GFF4524at 164.568 kb on - strand, within GFF4524at 164.568 kb on - strand, within GFF4524at 164.658 kb on - strand, within GFF4524at 164.684 kb on + strand, within GFF4524at 164.684 kb on + strand, within GFF4524at 164.684 kb on + strand, within GFF4524at 164.684 kb on + strand, within GFF4524at 164.685 kb on - strand, within GFF4524at 164.685 kb on - strand, within GFF4524at 164.685 kb on - strand, within GFF4524at 164.685 kb on - strand, within GFF4524at 164.685 kb on - strand, within GFF4524at 164.685 kb on - strand, within GFF4524at 164.708 kb on + strand, within GFF4524at 164.708 kb on + strand, within GFF4524at 164.709 kb on - strand, within GFF4524at 164.709 kb on - strand, within GFF4524at 165.220 kb on - strand, within GFF4525at 165.282 kb on - strandat 165.282 kb on - strandat 165.282 kb on - strandat 165.294 kb on + strandat 165.294 kb on + strandat 165.294 kb on + strandat 165.295 kb on - strandat 165.295 kb on - strandat 165.385 kb on + strand, within GFF4527at 165.385 kb on + strand, within GFF4527at 165.386 kb on - strand, within GFF4527at 165.592 kb on + strand, within GFF4527at 165.593 kb on - strand, within GFF4527at 165.796 kb on + strand, within GFF4527at 165.796 kb on + strand, within GFF4527at 165.796 kb on + strand, within GFF4527at 165.796 kb on + strand, within GFF4527at 165.797 kb on - strand, within GFF4527at 165.797 kb on - strand, within GFF4527at 165.797 kb on - strand, within GFF4527at 165.797 kb on - strand, within GFF4527at 165.797 kb on - strand, within GFF4527at 165.797 kb on - strand, within GFF4527at 165.797 kb on - strand, within GFF4527at 165.829 kb on + strand, within GFF4527at 165.829 kb on + strand, within GFF4527at 165.830 kb on - strand, within GFF4527at 165.830 kb on - strand, within GFF4527at 165.830 kb on - strand, within GFF4527at 165.830 kb on - strand, within GFF4527at 165.830 kb on - strand, within GFF4527at 165.830 kb on - strand, within GFF4527at 165.841 kb on + strand, within GFF4527

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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162,435 + GFF4523 0.20 +0.6
162,435 + GFF4523 0.20 -0.6
162,435 + GFF4523 0.20 -0.5
162,436 - GFF4523 0.20 -0.4
162,436 - GFF4523 0.20 -0.2
162,436 - GFF4523 0.20 -0.4
162,503 + GFF4523 0.25 +1.0
162,503 + GFF4523 0.25 -0.1
162,503 + GFF4523 0.25 -0.9
162,662 + GFF4523 0.39 -0.6
162,662 + GFF4523 0.39 +0.4
162,663 - GFF4523 0.39 -0.1
162,663 - GFF4523 0.39 -1.3
162,812 + GFF4523 0.51 +0.1
162,813 - GFF4523 0.51 +0.4
163,576 - -0.8
163,576 - -0.5
163,769 + GFF4524 0.23 +0.4
163,770 - GFF4524 0.23 -0.9
163,860 - GFF4524 0.29 -0.3
163,860 - GFF4524 0.29 -1.3
164,081 + GFF4524 0.44 -0.0
164,082 - GFF4524 0.44 -0.1
164,082 - GFF4524 0.44 -0.1
164,136 - GFF4524 0.48 -1.1
164,160 - GFF4524 0.49 -0.9
164,165 + GFF4524 0.50 -0.0
164,165 + GFF4524 0.50 +1.0
164,166 - GFF4524 0.50 +0.2
164,166 - GFF4524 0.50 -0.5
164,166 - GFF4524 0.50 +0.3
164,166 - GFF4524 0.50 -1.3
164,166 - GFF4524 0.50 -0.6
164,166 - GFF4524 0.50 +0.7
164,168 + GFF4524 0.50 +0.3
164,169 - GFF4524 0.50 +0.5
164,202 - GFF4524 0.52 -0.9
164,291 + GFF4524 0.58 +0.2
164,291 + GFF4524 0.58 +0.3
164,292 - GFF4524 0.58 +0.3
164,294 + GFF4524 0.58 -0.1
164,295 - GFF4524 0.58 +1.0
164,309 + GFF4524 0.59 -0.4
164,309 + GFF4524 0.59 +0.5
164,309 + GFF4524 0.59 +0.7
164,309 + GFF4524 0.59 +0.4
164,399 + GFF4524 0.66 -0.3
164,399 + GFF4524 0.66 +0.0
164,399 + GFF4524 0.66 +1.0
164,399 + GFF4524 0.66 -0.6
164,399 + GFF4524 0.66 -0.5
164,400 - GFF4524 0.66 -0.8
164,400 - GFF4524 0.66 +0.9
164,400 - GFF4524 0.66 +0.6
164,400 - GFF4524 0.66 +1.0
164,400 - GFF4524 0.66 +1.3
164,400 - GFF4524 0.66 +0.2
164,567 + GFF4524 0.77 -0.6
164,567 + GFF4524 0.77 +1.7
164,567 + GFF4524 0.77 -1.2
164,567 + GFF4524 0.77 +0.8
164,567 + GFF4524 0.77 -0.9
164,568 - GFF4524 0.77 +0.7
164,568 - GFF4524 0.77 +0.7
164,568 - GFF4524 0.77 -0.2
164,568 - GFF4524 0.77 +0.3
164,568 - GFF4524 0.77 -0.9
164,658 - GFF4524 0.83 +0.2
164,684 + GFF4524 0.85 -1.6
164,684 + GFF4524 0.85 -0.5
164,684 + GFF4524 0.85 +0.6
164,684 + GFF4524 0.85 +0.8
164,685 - GFF4524 0.85 -0.1
164,685 - GFF4524 0.85 +0.4
164,685 - GFF4524 0.85 -0.2
164,685 - GFF4524 0.85 +0.3
164,685 - GFF4524 0.85 -1.1
164,685 - GFF4524 0.85 +0.3
164,708 + GFF4524 0.86 +0.1
164,708 + GFF4524 0.86 +0.5
164,709 - GFF4524 0.87 -0.6
164,709 - GFF4524 0.87 -0.7
165,220 - GFF4525 0.71 +0.2
165,282 - -1.3
165,282 - -0.5
165,282 - -0.9
165,294 + +1.3
165,294 + -0.3
165,294 + +0.3
165,295 - -0.3
165,295 - +0.2
165,385 + GFF4527 0.11 -0.4
165,385 + GFF4527 0.11 -0.4
165,386 - GFF4527 0.12 +0.2
165,592 + GFF4527 0.38 -1.1
165,593 - GFF4527 0.38 -0.6
165,796 + GFF4527 0.64 -0.3
165,796 + GFF4527 0.64 -0.6
165,796 + GFF4527 0.64 -0.1
165,796 + GFF4527 0.64 +0.4
165,797 - GFF4527 0.64 -0.3
165,797 - GFF4527 0.64 -0.4
165,797 - GFF4527 0.64 -2.0
165,797 - GFF4527 0.64 -1.3
165,797 - GFF4527 0.64 -1.3
165,797 - GFF4527 0.64 -2.0
165,797 - GFF4527 0.64 +0.0
165,829 + GFF4527 0.68 -1.1
165,829 + GFF4527 0.68 -0.5
165,830 - GFF4527 0.68 -1.0
165,830 - GFF4527 0.68 -0.1
165,830 - GFF4527 0.68 -2.1
165,830 - GFF4527 0.68 -1.3
165,830 - GFF4527 0.68 -0.9
165,830 - GFF4527 0.68 +0.3
165,841 + GFF4527 0.70 -0.4

Or see this region's nucleotide sequence