Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF444 and GFF445 are separated by 22 nucleotides GFF445 and GFF446 overlap by 4 nucleotides GFF446 and GFF447 overlap by 4 nucleotides GFF447 and GFF448 overlap by 4 nucleotides
GFF444 - Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ Cob(I)alamin adenosyltransferase (EC 2.5.1.17), clustered with cobalamin synthesis, at 482,760 to 483,329
GFF444
GFF445 - Ferrichrome transport ATP-binding protein FhuC, at 483,352 to 484,128
GFF445
GFF446 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 484,125 to 485,150
GFF446
GFF447 - Vitamin B12 ABC transporter, substrate-binding protein BtuF, at 485,147 to 486,019
GFF447
GFF448 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 486,016 to 486,621
GFF448
Position (kb) 484
485
486 Strain fitness (log2 ratio) -2
-1
0
1
2 at 483.169 kb on + strand, within GFF444 at 483.169 kb on + strand, within GFF444 at 483.169 kb on + strand, within GFF444 at 483.169 kb on + strand, within GFF444 at 483.170 kb on - strand, within GFF444 at 483.170 kb on - strand, within GFF444 at 483.295 kb on + strand at 483.296 kb on - strand at 483.296 kb on - strand at 483.312 kb on - strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.344 kb on + strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.345 kb on - strand at 483.353 kb on + strand at 483.830 kb on + strand, within GFF445 at 483.830 kb on + strand, within GFF445 at 483.830 kb on + strand, within GFF445 at 483.830 kb on + strand, within GFF445 at 483.830 kb on + strand, within GFF445 at 483.831 kb on - strand, within GFF445 at 483.831 kb on - strand, within GFF445 at 483.831 kb on - strand, within GFF445 at 483.831 kb on - strand, within GFF445 at 483.831 kb on - strand, within GFF445 at 483.831 kb on - strand, within GFF445 at 483.831 kb on - strand, within GFF445 at 484.174 kb on + strand at 484.174 kb on + strand at 484.175 kb on - strand at 484.175 kb on - strand at 484.175 kb on - strand at 484.175 kb on - strand at 484.175 kb on - strand at 484.175 kb on - strand at 484.416 kb on + strand, within GFF446 at 484.417 kb on - strand, within GFF446 at 484.417 kb on - strand, within GFF446 at 484.599 kb on + strand, within GFF446 at 484.599 kb on + strand, within GFF446 at 484.599 kb on + strand, within GFF446 at 484.599 kb on + strand, within GFF446 at 484.600 kb on - strand, within GFF446 at 484.600 kb on - strand, within GFF446 at 484.874 kb on - strand, within GFF446 at 484.874 kb on - strand, within GFF446 at 485.367 kb on + strand, within GFF447 at 485.367 kb on + strand, within GFF447 at 485.367 kb on + strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.368 kb on - strand, within GFF447 at 485.497 kb on - strand, within GFF447 at 485.497 kb on - strand, within GFF447
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 483,169 + GFF444 0.72 +0.5 483,169 + GFF444 0.72 +1.4 483,169 + GFF444 0.72 +0.7 483,169 + GFF444 0.72 -0.6 483,170 - GFF444 0.72 -0.5 483,170 - GFF444 0.72 -1.2 483,295 + -1.3 483,296 - -1.1 483,296 - -2.7 483,312 - -1.1 483,344 + -0.4 483,344 + -1.2 483,344 + -0.7 483,344 + +0.4 483,344 + -1.3 483,344 + +0.7 483,344 + -1.7 483,344 + -0.4 483,344 + +0.1 483,345 - -0.6 483,345 - -0.2 483,345 - -1.6 483,345 - +0.3 483,345 - +0.1 483,345 - -0.6 483,345 - -1.1 483,345 - -1.0 483,345 - -0.8 483,345 - -0.1 483,345 - +0.3 483,345 - +0.1 483,345 - +0.4 483,345 - -1.9 483,353 + +0.5 483,830 + GFF445 0.62 -0.9 483,830 + GFF445 0.62 -0.3 483,830 + GFF445 0.62 -1.4 483,830 + GFF445 0.62 -0.5 483,830 + GFF445 0.62 -0.7 483,831 - GFF445 0.62 -0.5 483,831 - GFF445 0.62 -0.3 483,831 - GFF445 0.62 -0.1 483,831 - GFF445 0.62 +0.1 483,831 - GFF445 0.62 +0.5 483,831 - GFF445 0.62 -0.5 483,831 - GFF445 0.62 -0.3 484,174 + -0.3 484,174 + +1.1 484,175 - -0.5 484,175 - +0.8 484,175 - -0.2 484,175 - -0.3 484,175 - -1.3 484,175 - +1.7 484,416 + GFF446 0.28 -1.1 484,417 - GFF446 0.28 -0.7 484,417 - GFF446 0.28 -0.9 484,599 + GFF446 0.46 +0.9 484,599 + GFF446 0.46 -0.7 484,599 + GFF446 0.46 -0.5 484,599 + GFF446 0.46 +1.4 484,600 - GFF446 0.46 -0.7 484,600 - GFF446 0.46 +0.1 484,874 - GFF446 0.73 +0.7 484,874 - GFF446 0.73 +0.3 485,367 + GFF447 0.25 -0.2 485,367 + GFF447 0.25 -0.6 485,367 + GFF447 0.25 -1.9 485,368 - GFF447 0.25 -2.4 485,368 - GFF447 0.25 +2.1 485,368 - GFF447 0.25 -2.0 485,368 - GFF447 0.25 +0.8 485,497 - GFF447 0.40 -0.9 485,497 - GFF447 0.40 -0.2
Or see this region's nucleotide sequence