Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF440 and GFF441 overlap by 4 nucleotides GFF441 and GFF442 overlap by 4 nucleotides GFF442 and GFF443 overlap by 1 nucleotides GFF443 and GFF444 are separated by 54 nucleotides
GFF440 - CobN-like chelatase BtuS for metalloporphyrine salvage, at 477,183 to 481,196
GFF440
GFF441 - no description, at 481,193 to 481,546
GFF441
GFF442 - hypothetical transporter PduT for various metalloporphyrins, at 481,543 to 482,016
GFF442
GFF443 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 482,016 to 482,705
GFF443
GFF444 - Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ Cob(I)alamin adenosyltransferase (EC 2.5.1.17), clustered with cobalamin synthesis, at 482,760 to 483,329
GFF444
Position (kb) 481
482
483 Strain fitness (log2 ratio) -2
-1
0
1
2 at 480.649 kb on + strand, within GFF440 at 480.649 kb on + strand, within GFF440 at 480.649 kb on + strand, within GFF440 at 480.649 kb on + strand, within GFF440 at 480.649 kb on + strand, within GFF440 at 480.650 kb on - strand, within GFF440 at 480.650 kb on - strand, within GFF440 at 480.871 kb on + strand at 480.871 kb on + strand at 480.871 kb on + strand at 480.871 kb on + strand at 480.871 kb on + strand at 480.871 kb on + strand at 480.872 kb on - strand at 480.872 kb on - strand at 480.872 kb on - strand at 480.872 kb on - strand at 480.872 kb on - strand at 481.233 kb on + strand, within GFF441 at 481.233 kb on + strand, within GFF441 at 481.233 kb on + strand, within GFF441 at 481.233 kb on + strand, within GFF441 at 481.234 kb on - strand, within GFF441 at 481.234 kb on - strand, within GFF441 at 481.234 kb on - strand, within GFF441 at 481.234 kb on - strand, within GFF441 at 481.234 kb on - strand, within GFF441 at 481.613 kb on + strand, within GFF442 at 481.613 kb on + strand, within GFF442 at 481.613 kb on + strand, within GFF442 at 481.613 kb on + strand, within GFF442 at 481.613 kb on + strand, within GFF442 at 481.614 kb on - strand, within GFF442 at 481.614 kb on - strand, within GFF442 at 481.614 kb on - strand, within GFF442 at 481.614 kb on - strand, within GFF442 at 481.895 kb on + strand, within GFF442 at 481.895 kb on + strand, within GFF442 at 481.895 kb on + strand, within GFF442 at 481.895 kb on + strand, within GFF442 at 481.895 kb on + strand, within GFF442 at 481.895 kb on + strand, within GFF442 at 481.896 kb on - strand, within GFF442 at 481.896 kb on - strand, within GFF442 at 481.896 kb on - strand, within GFF442 at 481.896 kb on - strand, within GFF442 at 481.922 kb on + strand, within GFF442 at 481.922 kb on + strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 481.923 kb on - strand, within GFF442 at 482.146 kb on + strand, within GFF443 at 482.147 kb on - strand, within GFF443 at 482.662 kb on + strand at 482.790 kb on - strand at 482.790 kb on - strand at 482.790 kb on - strand at 482.835 kb on - strand, within GFF444 at 482.946 kb on + strand, within GFF444 at 482.947 kb on + strand, within GFF444 at 482.947 kb on + strand, within GFF444 at 482.947 kb on + strand, within GFF444 at 482.948 kb on - strand, within GFF444 at 482.948 kb on - strand, within GFF444 at 482.948 kb on - strand, within GFF444 at 482.948 kb on - strand, within GFF444 at 482.959 kb on + strand, within GFF444 at 482.960 kb on - strand, within GFF444 at 482.960 kb on - strand, within GFF444
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 480,649 + GFF440 0.86 +0.2 480,649 + GFF440 0.86 -0.2 480,649 + GFF440 0.86 -0.8 480,649 + GFF440 0.86 +0.7 480,649 + GFF440 0.86 -0.1 480,650 - GFF440 0.86 -0.3 480,650 - GFF440 0.86 -0.4 480,871 + +0.1 480,871 + -0.3 480,871 + -0.3 480,871 + +0.4 480,871 + -0.9 480,871 + +0.2 480,872 - +1.2 480,872 - -0.5 480,872 - +0.6 480,872 - -1.6 480,872 - -1.8 481,233 + GFF441 0.11 -0.2 481,233 + GFF441 0.11 -1.0 481,233 + GFF441 0.11 -1.3 481,233 + GFF441 0.11 +0.4 481,234 - GFF441 0.12 +0.0 481,234 - GFF441 0.12 -0.2 481,234 - GFF441 0.12 +0.1 481,234 - GFF441 0.12 -2.1 481,234 - GFF441 0.12 +0.8 481,613 + GFF442 0.15 +0.4 481,613 + GFF442 0.15 +0.3 481,613 + GFF442 0.15 -0.8 481,613 + GFF442 0.15 -0.6 481,613 + GFF442 0.15 -1.1 481,614 - GFF442 0.15 -0.2 481,614 - GFF442 0.15 -1.1 481,614 - GFF442 0.15 +0.2 481,614 - GFF442 0.15 +1.9 481,895 + GFF442 0.74 +0.5 481,895 + GFF442 0.74 +1.6 481,895 + GFF442 0.74 +0.4 481,895 + GFF442 0.74 -0.1 481,895 + GFF442 0.74 -0.9 481,895 + GFF442 0.74 -1.6 481,896 - GFF442 0.74 -0.3 481,896 - GFF442 0.74 -0.6 481,896 - GFF442 0.74 +0.5 481,896 - GFF442 0.74 +0.6 481,922 + GFF442 0.80 +0.1 481,922 + GFF442 0.80 -0.7 481,923 - GFF442 0.80 -0.8 481,923 - GFF442 0.80 +0.5 481,923 - GFF442 0.80 +0.5 481,923 - GFF442 0.80 +0.1 481,923 - GFF442 0.80 -0.3 481,923 - GFF442 0.80 -0.8 481,923 - GFF442 0.80 +0.2 481,923 - GFF442 0.80 +2.2 482,146 + GFF443 0.19 +2.0 482,147 - GFF443 0.19 -0.6 482,662 + -0.0 482,790 - +0.1 482,790 - -1.1 482,790 - +1.0 482,835 - GFF444 0.13 -1.1 482,946 + GFF444 0.33 -1.3 482,947 + GFF444 0.33 -0.2 482,947 + GFF444 0.33 -1.0 482,947 + GFF444 0.33 +0.1 482,948 - GFF444 0.33 -0.5 482,948 - GFF444 0.33 +0.9 482,948 - GFF444 0.33 -0.0 482,948 - GFF444 0.33 -0.9 482,959 + GFF444 0.35 +0.0 482,960 - GFF444 0.35 +0.6 482,960 - GFF444 0.35 +0.4
Or see this region's nucleotide sequence