Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4355 and GFF4356 are separated by 162 nucleotides GFF4356 and GFF4357 overlap by 4 nucleotides GFF4357 and GFF4358 are separated by 49 nucleotides
GFF4355 - Alcohol dehydrogenase (EC 1.1.1.1), at 278,086 to 279,123
GFF4355
GFF4356 - Alpha-methylacyl-CoA racemase (EC 5.1.99.4), at 279,286 to 280,458
GFF4356
GFF4357 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 280,455 to 281,240
GFF4357
GFF4358 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 281,290 to 282,078
GFF4358
Position (kb) 279
280
281 Strain fitness (log2 ratio) -2
-1
0
1 at 278.465 kb on + strand, within GFF4355 at 278.747 kb on + strand, within GFF4355 at 278.855 kb on + strand, within GFF4355 at 278.881 kb on + strand, within GFF4355 at 278.882 kb on - strand, within GFF4355 at 278.882 kb on - strand, within GFF4355 at 278.993 kb on + strand, within GFF4355 at 278.994 kb on - strand, within GFF4355 at 279.138 kb on - strand at 279.694 kb on + strand, within GFF4356 at 279.694 kb on + strand, within GFF4356 at 279.793 kb on + strand, within GFF4356 at 279.794 kb on - strand, within GFF4356 at 279.985 kb on + strand, within GFF4356 at 279.985 kb on + strand, within GFF4356 at 279.985 kb on + strand, within GFF4356 at 280.006 kb on + strand, within GFF4356 at 280.007 kb on - strand, within GFF4356 at 280.007 kb on - strand, within GFF4356 at 280.061 kb on + strand, within GFF4356 at 280.370 kb on + strand at 280.660 kb on - strand, within GFF4357
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 278,465 + GFF4355 0.37 +0.7 278,747 + GFF4355 0.64 -0.6 278,855 + GFF4355 0.74 +0.0 278,881 + GFF4355 0.77 -0.8 278,882 - GFF4355 0.77 -0.0 278,882 - GFF4355 0.77 -0.3 278,993 + GFF4355 0.87 -0.8 278,994 - GFF4355 0.87 +1.7 279,138 - -0.5 279,694 + GFF4356 0.35 -0.5 279,694 + GFF4356 0.35 -0.3 279,793 + GFF4356 0.43 +0.7 279,794 - GFF4356 0.43 -0.1 279,985 + GFF4356 0.60 -2.0 279,985 + GFF4356 0.60 +0.4 279,985 + GFF4356 0.60 +0.2 280,006 + GFF4356 0.61 +0.4 280,007 - GFF4356 0.61 -0.1 280,007 - GFF4356 0.61 +1.7 280,061 + GFF4356 0.66 -0.3 280,370 + +1.7 280,660 - GFF4357 0.26 +0.7
Or see this region's nucleotide sequence