Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4307 and GFF4308 are separated by 48 nucleotides GFF4308 and GFF4309 are separated by 1 nucleotides GFF4309 and GFF4310 overlap by 4 nucleotides
GFF4307 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 227,069 to 228,109
GFF4307
GFF4308 - hypothetical protein, at 228,158 to 229,363
GFF4308
GFF4309 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 229,365 to 230,201
GFF4309
GFF4310 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 230,198 to 232,054
GFF4310
Position (kb) 228
229
230 Strain fitness (log2 ratio) -2
-1
0
1
2 at 227.195 kb on + strand, within GFF4307 at 227.319 kb on - strand, within GFF4307 at 227.357 kb on + strand, within GFF4307 at 227.358 kb on - strand, within GFF4307 at 227.786 kb on + strand, within GFF4307 at 227.787 kb on - strand, within GFF4307 at 227.792 kb on + strand, within GFF4307 at 227.793 kb on - strand, within GFF4307 at 227.813 kb on + strand, within GFF4307 at 227.814 kb on - strand, within GFF4307 at 228.142 kb on - strand at 228.342 kb on - strand, within GFF4308 at 228.698 kb on + strand, within GFF4308 at 228.699 kb on - strand, within GFF4308 at 228.704 kb on + strand, within GFF4308 at 228.704 kb on + strand, within GFF4308 at 228.864 kb on + strand, within GFF4308 at 228.864 kb on + strand, within GFF4308 at 228.875 kb on + strand, within GFF4308 at 228.875 kb on + strand, within GFF4308 at 229.022 kb on + strand, within GFF4308 at 229.022 kb on - strand, within GFF4308 at 229.119 kb on - strand, within GFF4308 at 229.304 kb on + strand at 229.304 kb on + strand at 229.305 kb on - strand at 229.305 kb on - strand at 229.310 kb on + strand at 229.311 kb on - strand at 229.867 kb on + strand, within GFF4309 at 229.867 kb on + strand, within GFF4309 at 229.868 kb on - strand, within GFF4309 at 230.016 kb on + strand, within GFF4309 at 230.315 kb on + strand at 230.315 kb on + strand at 230.316 kb on - strand at 230.316 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 227,195 + GFF4307 0.12 +0.5 227,319 - GFF4307 0.24 -1.8 227,357 + GFF4307 0.28 +0.7 227,358 - GFF4307 0.28 -0.1 227,786 + GFF4307 0.69 +0.7 227,787 - GFF4307 0.69 -1.9 227,792 + GFF4307 0.69 -1.0 227,793 - GFF4307 0.70 +0.1 227,813 + GFF4307 0.71 -0.7 227,814 - GFF4307 0.72 -1.0 228,142 - -0.8 228,342 - GFF4308 0.15 +0.8 228,698 + GFF4308 0.45 -0.7 228,699 - GFF4308 0.45 +0.5 228,704 + GFF4308 0.45 +1.7 228,704 + GFF4308 0.45 -0.2 228,864 + GFF4308 0.59 +0.1 228,864 + GFF4308 0.59 +1.1 228,875 + GFF4308 0.59 +1.3 228,875 + GFF4308 0.59 +0.0 229,022 + GFF4308 0.72 -1.7 229,022 - GFF4308 0.72 -0.1 229,119 - GFF4308 0.80 +0.0 229,304 + -0.1 229,304 + -0.5 229,305 - -1.8 229,305 - -1.2 229,310 + -0.0 229,311 - -1.5 229,867 + GFF4309 0.60 -0.1 229,867 + GFF4309 0.60 -1.2 229,868 - GFF4309 0.60 +0.7 230,016 + GFF4309 0.78 -1.0 230,315 + -1.2 230,315 + +0.2 230,316 - +2.7 230,316 - -0.3
Or see this region's nucleotide sequence