Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4226 and GFF4227 are separated by 6 nucleotides GFF4227 and GFF4228 are separated by 21 nucleotides GFF4228 and GFF4229 overlap by 4 nucleotides
GFF4226 - Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84), at 137,725 to 138,714
GFF4226
GFF4227 - Thiamine pyrophosphate-requiring enzymes, at 138,721 to 140,361
GFF4227
GFF4228 - 2-hydroxychromene-2-carboxylate isomerase, at 140,383 to 141,003
GFF4228
GFF4229 - Transcriptional regulator, at 141,000 to 141,641
GFF4229
Position (kb) 138
139
140
141 Strain fitness (log2 ratio) -2
-1
0
1
2 at 137.833 kb on + strand, within GFF4226 at 137.833 kb on + strand, within GFF4226 at 137.833 kb on + strand, within GFF4226 at 137.834 kb on - strand, within GFF4226 at 137.848 kb on + strand, within GFF4226 at 138.155 kb on + strand, within GFF4226 at 138.155 kb on + strand, within GFF4226 at 138.156 kb on - strand, within GFF4226 at 138.156 kb on - strand, within GFF4226 at 138.156 kb on - strand, within GFF4226 at 138.283 kb on + strand, within GFF4226 at 138.283 kb on + strand, within GFF4226 at 138.284 kb on - strand, within GFF4226 at 139.618 kb on + strand, within GFF4227 at 139.618 kb on + strand, within GFF4227 at 139.618 kb on + strand, within GFF4227 at 139.619 kb on - strand, within GFF4227 at 139.619 kb on - strand, within GFF4227 at 140.122 kb on + strand, within GFF4227 at 140.405 kb on - strand at 140.405 kb on - strand at 140.407 kb on + strand at 140.407 kb on + strand at 140.516 kb on - strand, within GFF4228 at 140.545 kb on + strand, within GFF4228 at 140.854 kb on + strand, within GFF4228 at 140.855 kb on - strand, within GFF4228 at 140.855 kb on - strand, within GFF4228 at 140.864 kb on - strand, within GFF4228 at 140.908 kb on + strand, within GFF4228 at 140.909 kb on - strand, within GFF4228 at 141.002 kb on - strand at 141.184 kb on + strand, within GFF4229 at 141.185 kb on - strand, within GFF4229 at 141.278 kb on - strand, within GFF4229 at 141.278 kb on - strand, within GFF4229 at 141.278 kb on - strand, within GFF4229 at 141.328 kb on + strand, within GFF4229
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 137,833 + GFF4226 0.11 -1.0 137,833 + GFF4226 0.11 -0.0 137,833 + GFF4226 0.11 -2.6 137,834 - GFF4226 0.11 +1.0 137,848 + GFF4226 0.12 +0.1 138,155 + GFF4226 0.43 -1.1 138,155 + GFF4226 0.43 -0.8 138,156 - GFF4226 0.44 -0.9 138,156 - GFF4226 0.44 -0.4 138,156 - GFF4226 0.44 -0.1 138,283 + GFF4226 0.56 -1.3 138,283 + GFF4226 0.56 +0.6 138,284 - GFF4226 0.56 -0.6 139,618 + GFF4227 0.55 +0.5 139,618 + GFF4227 0.55 -1.3 139,618 + GFF4227 0.55 +0.3 139,619 - GFF4227 0.55 +0.7 139,619 - GFF4227 0.55 +1.0 140,122 + GFF4227 0.85 -0.7 140,405 - -0.1 140,405 - -0.3 140,407 + -0.4 140,407 + +2.0 140,516 - GFF4228 0.21 -0.3 140,545 + GFF4228 0.26 -0.0 140,854 + GFF4228 0.76 -0.2 140,855 - GFF4228 0.76 +0.7 140,855 - GFF4228 0.76 -0.5 140,864 - GFF4228 0.77 -1.8 140,908 + GFF4228 0.85 +0.4 140,909 - GFF4228 0.85 -0.3 141,002 - +0.1 141,184 + GFF4229 0.29 -0.7 141,185 - GFF4229 0.29 +0.7 141,278 - GFF4229 0.43 -0.1 141,278 - GFF4229 0.43 +1.1 141,278 - GFF4229 0.43 -0.4 141,328 + GFF4229 0.51 +0.3
Or see this region's nucleotide sequence