Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4156 and GFF4157 overlap by 4 nucleotides GFF4157 and GFF4158 are separated by 10 nucleotides GFF4158 and GFF4159 overlap by 4 nucleotides GFF4159 and GFF4160 overlap by 4 nucleotides
GFF4156 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 62,125 to 62,865
GFF4156
GFF4157 - hypothetical protein, at 62,862 to 63,812
GFF4157
GFF4158 - hypothetical protein, at 63,823 to 64,251
GFF4158
GFF4159 - no description, at 64,248 to 64,967
GFF4159
GFF4160 - Demethylmenaquinone methyltransferase-like protein, at 64,964 to 65,719
GFF4160
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1 at 62.887 kb on - strand at 62.887 kb on - strand at 62.994 kb on + strand, within GFF4157 at 62.995 kb on - strand, within GFF4157 at 62.995 kb on - strand, within GFF4157 at 63.910 kb on + strand, within GFF4158 at 63.910 kb on + strand, within GFF4158 at 63.910 kb on + strand, within GFF4158 at 63.911 kb on - strand, within GFF4158 at 64.057 kb on + strand, within GFF4158 at 64.057 kb on + strand, within GFF4158 at 64.057 kb on + strand, within GFF4158 at 64.058 kb on - strand, within GFF4158 at 64.058 kb on - strand, within GFF4158 at 64.058 kb on - strand, within GFF4158 at 64.058 kb on - strand, within GFF4158 at 64.058 kb on - strand, within GFF4158 at 64.058 kb on - strand, within GFF4158 at 64.561 kb on + strand, within GFF4159 at 64.562 kb on + strand, within GFF4159 at 64.563 kb on - strand, within GFF4159 at 64.563 kb on - strand, within GFF4159 at 65.069 kb on + strand, within GFF4160 at 65.070 kb on - strand, within GFF4160 at 65.126 kb on + strand, within GFF4160 at 65.126 kb on + strand, within GFF4160 at 65.177 kb on + strand, within GFF4160 at 65.178 kb on - strand, within GFF4160
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 62,887 - +0.7 62,887 - +0.3 62,994 + GFF4157 0.14 +0.0 62,995 - GFF4157 0.14 -1.6 62,995 - GFF4157 0.14 -0.1 63,910 + GFF4158 0.20 -1.8 63,910 + GFF4158 0.20 -0.3 63,910 + GFF4158 0.20 -0.3 63,911 - GFF4158 0.21 +0.2 64,057 + GFF4158 0.55 -0.7 64,057 + GFF4158 0.55 -0.6 64,057 + GFF4158 0.55 +0.0 64,058 - GFF4158 0.55 -1.4 64,058 - GFF4158 0.55 +0.3 64,058 - GFF4158 0.55 -1.1 64,058 - GFF4158 0.55 -2.4 64,058 - GFF4158 0.55 -1.9 64,058 - GFF4158 0.55 +0.7 64,561 + GFF4159 0.43 +0.8 64,562 + GFF4159 0.44 +0.2 64,563 - GFF4159 0.44 +0.2 64,563 - GFF4159 0.44 +0.4 65,069 + GFF4160 0.14 -0.6 65,070 - GFF4160 0.14 -0.7 65,126 + GFF4160 0.21 -0.4 65,126 + GFF4160 0.21 +0.2 65,177 + GFF4160 0.28 -0.7 65,178 - GFF4160 0.28 +0.1
Or see this region's nucleotide sequence