Strain Fitness in Variovorax sp. SCN45 around GFF411

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF409 and GFF410 are separated by 14 nucleotidesGFF410 and GFF411 overlap by 4 nucleotidesGFF411 and GFF412 are separated by 119 nucleotides GFF409 - Osmolarity sensory histidine kinase EnvZ, at 443,143 to 444,564 GFF409 GFF410 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 444,579 to 445,067 GFF410 GFF411 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60), at 445,064 to 445,786 GFF411 GFF412 - Transcription-repair coupling factor, at 445,906 to 449,388 GFF412 Position (kb) 445 446Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 444.097 kb on + strand, within GFF409at 444.097 kb on + strand, within GFF409at 444.112 kb on + strand, within GFF409at 444.112 kb on + strand, within GFF409at 444.112 kb on + strand, within GFF409at 444.113 kb on - strand, within GFF409at 444.288 kb on - strand, within GFF409at 444.491 kb on + strandat 444.491 kb on + strandat 444.491 kb on + strandat 444.491 kb on + strandat 444.492 kb on - strandat 444.492 kb on - strandat 444.492 kb on - strandat 444.492 kb on - strandat 444.492 kb on - strandat 444.513 kb on + strandat 444.513 kb on + strandat 444.513 kb on + strandat 444.514 kb on - strandat 444.514 kb on - strandat 444.514 kb on - strandat 444.514 kb on - strandat 444.514 kb on - strandat 444.514 kb on - strandat 444.514 kb on - strandat 445.797 kb on - strandat 445.841 kb on + strandat 445.841 kb on + strandat 445.896 kb on + strandat 446.134 kb on + strandat 446.246 kb on - strandat 446.278 kb on + strand, within GFF412at 446.278 kb on + strand, within GFF412at 446.278 kb on + strand, within GFF412at 446.278 kb on + strand, within GFF412at 446.279 kb on - strand, within GFF412at 446.279 kb on - strand, within GFF412at 446.387 kb on - strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.635 kb on + strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.636 kb on - strand, within GFF412at 446.677 kb on + strand, within GFF412at 446.678 kb on - strand, within GFF412at 446.678 kb on - strand, within GFF412at 446.678 kb on - strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.680 kb on + strand, within GFF412at 446.681 kb on - strand, within GFF412at 446.681 kb on - strand, within GFF412at 446.681 kb on - strand, within GFF412at 446.681 kb on - strand, within GFF412at 446.681 kb on - strand, within GFF412at 446.681 kb on - strand, within GFF412at 446.681 kb on - strand, within GFF412at 446.723 kb on - strand, within GFF412at 446.723 kb on - strand, within GFF412at 446.723 kb on - strand, within GFF412at 446.723 kb on - strand, within GFF412

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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444,097 + GFF409 0.67 -0.7
444,097 + GFF409 0.67 +0.3
444,112 + GFF409 0.68 -1.0
444,112 + GFF409 0.68 -2.2
444,112 + GFF409 0.68 +0.3
444,113 - GFF409 0.68 -2.1
444,288 - GFF409 0.81 +0.3
444,491 + -0.3
444,491 + -0.7
444,491 + -2.6
444,491 + +1.6
444,492 - -0.3
444,492 - -0.2
444,492 - -0.3
444,492 - -3.2
444,492 - -0.3
444,513 + +1.5
444,513 + +1.8
444,513 + -2.3
444,514 - +1.3
444,514 - +2.8
444,514 - -0.7
444,514 - +0.7
444,514 - -2.5
444,514 - +1.1
444,514 - +0.5
445,797 - +0.4
445,841 + +0.7
445,841 + -0.1
445,896 + +0.6
446,134 + -0.5
446,246 - -1.1
446,278 + GFF412 0.11 -0.9
446,278 + GFF412 0.11 -1.1
446,278 + GFF412 0.11 -0.4
446,278 + GFF412 0.11 -1.2
446,279 - GFF412 0.11 -0.8
446,279 - GFF412 0.11 +0.8
446,387 - GFF412 0.14 -1.6
446,635 + GFF412 0.21 -0.6
446,635 + GFF412 0.21 +0.0
446,635 + GFF412 0.21 -0.1
446,635 + GFF412 0.21 +1.0
446,635 + GFF412 0.21 -0.2
446,635 + GFF412 0.21 -0.3
446,635 + GFF412 0.21 -0.3
446,635 + GFF412 0.21 +1.3
446,635 + GFF412 0.21 -0.6
446,635 + GFF412 0.21 -0.7
446,636 - GFF412 0.21 +0.4
446,636 - GFF412 0.21 +1.4
446,636 - GFF412 0.21 -0.3
446,636 - GFF412 0.21 +0.8
446,636 - GFF412 0.21 -0.3
446,636 - GFF412 0.21 -0.4
446,636 - GFF412 0.21 -0.4
446,636 - GFF412 0.21 -0.8
446,636 - GFF412 0.21 -0.5
446,636 - GFF412 0.21 -2.1
446,636 - GFF412 0.21 -0.5
446,636 - GFF412 0.21 -0.2
446,636 - GFF412 0.21 +0.8
446,677 + GFF412 0.22 +0.8
446,678 - GFF412 0.22 +1.3
446,678 - GFF412 0.22 +1.0
446,678 - GFF412 0.22 -1.4
446,680 + GFF412 0.22 -0.2
446,680 + GFF412 0.22 -1.7
446,680 + GFF412 0.22 +0.3
446,680 + GFF412 0.22 -0.1
446,680 + GFF412 0.22 -0.1
446,680 + GFF412 0.22 -1.0
446,680 + GFF412 0.22 +0.4
446,680 + GFF412 0.22 -2.2
446,681 - GFF412 0.22 -0.2
446,681 - GFF412 0.22 +0.8
446,681 - GFF412 0.22 +0.4
446,681 - GFF412 0.22 -0.8
446,681 - GFF412 0.22 -0.9
446,681 - GFF412 0.22 -0.4
446,681 - GFF412 0.22 +0.3
446,723 - GFF412 0.23 +0.2
446,723 - GFF412 0.23 -0.0
446,723 - GFF412 0.23 -1.9
446,723 - GFF412 0.23 +0.0

Or see this region's nucleotide sequence