Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4041 and GFF4042 are separated by 514 nucleotides GFF4042 and GFF4043 overlap by 4 nucleotides
GFF4041 - Lipid A phosphoethanolamine transferase, putative, at 251,164 to 252,579
GFF4041
GFF4042 - Copper-sensing two-component system response regulator CusR, at 253,094 to 253,777
GFF4042
GFF4043 - Copper sensory histidine kinase CusS, at 253,774 to 255,102
GFF4043
Position (kb)
253
254 Strain fitness (log2 ratio)
-1
0
1
2 at 252.154 kb on - strand, within GFF4041 at 252.221 kb on + strand, within GFF4041 at 252.236 kb on + strand, within GFF4041 at 252.236 kb on + strand, within GFF4041 at 252.236 kb on + strand, within GFF4041 at 252.237 kb on - strand, within GFF4041 at 252.237 kb on - strand, within GFF4041 at 252.237 kb on - strand, within GFF4041 at 252.237 kb on - strand, within GFF4041 at 252.237 kb on - strand, within GFF4041 at 252.303 kb on - strand, within GFF4041 at 252.309 kb on + strand, within GFF4041 at 252.309 kb on + strand, within GFF4041 at 252.551 kb on + strand at 252.551 kb on + strand at 252.552 kb on - strand at 252.552 kb on - strand at 252.583 kb on + strand at 252.584 kb on - strand at 252.746 kb on - strand at 252.746 kb on - strand at 252.981 kb on - strand at 252.981 kb on - strand at 252.990 kb on + strand at 252.990 kb on + strand at 252.990 kb on + strand at 252.990 kb on + strand at 252.991 kb on - strand at 253.057 kb on + strand at 253.057 kb on + strand at 253.137 kb on - strand at 253.137 kb on - strand at 253.226 kb on + strand, within GFF4042 at 253.227 kb on - strand, within GFF4042 at 253.383 kb on - strand, within GFF4042 at 253.383 kb on - strand, within GFF4042 at 253.747 kb on - strand at 253.747 kb on - strand at 253.915 kb on + strand, within GFF4043 at 253.918 kb on - strand, within GFF4043 at 254.054 kb on - strand, within GFF4043 at 254.054 kb on - strand, within GFF4043
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 252,154 - GFF4041 0.70 +0.3 252,221 + GFF4041 0.75 -0.0 252,236 + GFF4041 0.76 -0.4 252,236 + GFF4041 0.76 -1.1 252,236 + GFF4041 0.76 -0.4 252,237 - GFF4041 0.76 +1.6 252,237 - GFF4041 0.76 -0.7 252,237 - GFF4041 0.76 +0.3 252,237 - GFF4041 0.76 -0.4 252,237 - GFF4041 0.76 -0.2 252,303 - GFF4041 0.80 +0.4 252,309 + GFF4041 0.81 +1.0 252,309 + GFF4041 0.81 +0.2 252,551 + -1.5 252,551 + -1.6 252,552 - +0.5 252,552 - -0.2 252,583 + -0.1 252,584 - +0.2 252,746 - +2.1 252,746 - +0.6 252,981 - +0.4 252,981 - +1.2 252,990 + -0.4 252,990 + -0.3 252,990 + -1.1 252,990 + +0.6 252,991 - +0.0 253,057 + -0.0 253,057 + +0.2 253,137 - -0.1 253,137 - +0.0 253,226 + GFF4042 0.19 -0.3 253,227 - GFF4042 0.19 +0.6 253,383 - GFF4042 0.42 -0.0 253,383 - GFF4042 0.42 +0.6 253,747 - -1.1 253,747 - +0.8 253,915 + GFF4043 0.11 -0.6 253,918 - GFF4043 0.11 -0.0 254,054 - GFF4043 0.21 +1.1 254,054 - GFF4043 0.21 +0.1
Or see this region's nucleotide sequence