Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3978 and GFF3979 are separated by 28 nucleotides GFF3979 and GFF3980 overlap by 4 nucleotides GFF3980 and GFF3981 are separated by 43 nucleotides
GFF3978 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 181,348 to 182,493
GFF3978
GFF3979 - 3-phenylpropionate dioxygenase alpha subunit (EC 1.14.1.-), at 182,522 to 183,847
GFF3979
GFF3980 - 3-phenylpropionate dioxygenase beta subunit (EC 1.14.1.-), at 183,844 to 184,308
GFF3980
GFF3981 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 184,352 to 185,515
GFF3981
Position (kb) 182
183
184 Strain fitness (log2 ratio) -1
0
1
2 at 181.859 kb on + strand, within GFF3978 at 181.859 kb on + strand, within GFF3978 at 181.860 kb on - strand, within GFF3978 at 181.860 kb on - strand, within GFF3978 at 181.864 kb on + strand, within GFF3978 at 182.029 kb on + strand, within GFF3978 at 182.030 kb on - strand, within GFF3978 at 182.030 kb on - strand, within GFF3978 at 182.053 kb on + strand, within GFF3978 at 182.054 kb on - strand, within GFF3978 at 182.069 kb on - strand, within GFF3978 at 182.272 kb on + strand, within GFF3978 at 182.272 kb on + strand, within GFF3978 at 182.273 kb on - strand, within GFF3978 at 182.281 kb on + strand, within GFF3978 at 182.401 kb on + strand at 182.484 kb on - strand at 182.531 kb on + strand at 182.531 kb on + strand at 182.531 kb on + strand at 182.858 kb on + strand, within GFF3979 at 183.026 kb on + strand, within GFF3979 at 183.140 kb on + strand, within GFF3979 at 183.140 kb on + strand, within GFF3979 at 183.141 kb on - strand, within GFF3979 at 183.264 kb on + strand, within GFF3979 at 183.265 kb on - strand, within GFF3979 at 183.369 kb on - strand, within GFF3979 at 183.386 kb on + strand, within GFF3979 at 183.435 kb on + strand, within GFF3979 at 183.435 kb on + strand, within GFF3979 at 183.583 kb on - strand, within GFF3979 at 183.714 kb on + strand, within GFF3979 at 183.714 kb on + strand, within GFF3979 at 183.941 kb on - strand, within GFF3980 at 184.156 kb on + strand, within GFF3980 at 184.347 kb on - strand at 184.526 kb on + strand, within GFF3981 at 184.527 kb on - strand, within GFF3981 at 184.844 kb on + strand, within GFF3981 at 184.844 kb on + strand, within GFF3981
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 181,859 + GFF3978 0.45 -0.3 181,859 + GFF3978 0.45 +2.2 181,860 - GFF3978 0.45 -0.4 181,860 - GFF3978 0.45 +0.4 181,864 + GFF3978 0.45 -1.4 182,029 + GFF3978 0.59 +0.0 182,030 - GFF3978 0.60 -0.2 182,030 - GFF3978 0.60 -0.1 182,053 + GFF3978 0.62 +1.0 182,054 - GFF3978 0.62 -0.8 182,069 - GFF3978 0.63 -0.2 182,272 + GFF3978 0.81 +0.4 182,272 + GFF3978 0.81 -0.6 182,273 - GFF3978 0.81 +1.6 182,281 + GFF3978 0.81 +0.3 182,401 + -0.4 182,484 - -0.0 182,531 + +0.1 182,531 + -0.7 182,531 + -0.3 182,858 + GFF3979 0.25 -0.2 183,026 + GFF3979 0.38 +0.1 183,140 + GFF3979 0.47 -0.0 183,140 + GFF3979 0.47 -0.1 183,141 - GFF3979 0.47 -0.2 183,264 + GFF3979 0.56 -0.6 183,265 - GFF3979 0.56 -0.1 183,369 - GFF3979 0.64 -0.0 183,386 + GFF3979 0.65 +1.1 183,435 + GFF3979 0.69 +1.2 183,435 + GFF3979 0.69 -0.2 183,583 - GFF3979 0.80 -0.4 183,714 + GFF3979 0.90 +0.1 183,714 + GFF3979 0.90 -0.2 183,941 - GFF3980 0.21 -0.2 184,156 + GFF3980 0.67 -0.1 184,347 - -0.3 184,526 + GFF3981 0.15 +0.8 184,527 - GFF3981 0.15 -0.0 184,844 + GFF3981 0.42 -0.4 184,844 + GFF3981 0.42 -1.4
Or see this region's nucleotide sequence