Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3969 and GFF3970 are separated by 20 nucleotides GFF3970 and GFF3971 overlap by 1 nucleotides GFF3971 and GFF3972 are separated by 14 nucleotides
GFF3969 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 171,688 to 172,680
GFF3969
GFF3970 - Ring hydroxylating dioxygenase, alpha subunit (EC 1.14.12.13), at 172,701 to 174,014
GFF3970
GFF3971 - Aromatic-ring-hydroxylating dioxygenase, beta subunit (EC 1.14.12.3), at 174,014 to 174,505
GFF3971
GFF3972 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 174,520 to 176,148
GFF3972
Position (kb) 172
173
174
175 Strain fitness (log2 ratio) -2
-1
0
1 at 171.919 kb on + strand, within GFF3969 at 171.919 kb on + strand, within GFF3969 at 172.294 kb on + strand, within GFF3969 at 172.634 kb on + strand at 172.634 kb on + strand at 172.635 kb on - strand at 172.710 kb on + strand at 172.773 kb on + strand at 172.774 kb on - strand at 173.082 kb on + strand, within GFF3970 at 173.206 kb on - strand, within GFF3970 at 173.547 kb on + strand, within GFF3970 at 173.547 kb on + strand, within GFF3970 at 173.548 kb on - strand, within GFF3970 at 173.548 kb on - strand, within GFF3970 at 173.784 kb on + strand, within GFF3970 at 173.784 kb on + strand, within GFF3970 at 173.784 kb on + strand, within GFF3970 at 173.784 kb on + strand, within GFF3970 at 173.785 kb on - strand, within GFF3970 at 174.387 kb on - strand, within GFF3971 at 174.700 kb on + strand, within GFF3972
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 171,919 + GFF3969 0.23 +0.8 171,919 + GFF3969 0.23 -0.5 172,294 + GFF3969 0.61 -1.4 172,634 + -0.0 172,634 + -0.2 172,635 - -0.3 172,710 + -0.0 172,773 + -0.3 172,774 - +0.3 173,082 + GFF3970 0.29 -0.1 173,206 - GFF3970 0.38 -1.0 173,547 + GFF3970 0.64 +0.3 173,547 + GFF3970 0.64 +0.5 173,548 - GFF3970 0.64 -0.9 173,548 - GFF3970 0.64 -0.4 173,784 + GFF3970 0.82 -1.9 173,784 + GFF3970 0.82 -2.3 173,784 + GFF3970 0.82 -0.0 173,784 + GFF3970 0.82 -1.1 173,785 - GFF3970 0.82 -1.2 174,387 - GFF3971 0.76 +0.6 174,700 + GFF3972 0.11 -0.0
Or see this region's nucleotide sequence