Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3845 and GFF3846 are separated by 6 nucleotides GFF3846 and GFF3847 are separated by 272 nucleotides GFF3847 and GFF3848 overlap by 8 nucleotides GFF3848 and GFF3849 are separated by 21 nucleotides
GFF3845 - Ribonuclease E (EC 3.1.26.12), at 44,185 to 47,391
GFF3845
GFF3846 - hypothetical protein, at 47,398 to 47,661
GFF3846
GFF3847 - LSU rRNA pseudouridine(955/2504/2580) synthase (EC 5.4.99.24), at 47,934 to 49,046
GFF3847
GFF3848 - Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C, at 49,039 to 49,710
GFF3848
GFF3849 - Iron-sulfur cluster-binding protein, Rieske family, at 49,732 to 50,100
GFF3849
Position (kb)
47
48
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 47.406 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.408 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.412 kb on - strand at 47.420 kb on - strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.959 kb on + strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 47.960 kb on - strand at 48.135 kb on + strand, within GFF3847 at 48.136 kb on - strand, within GFF3847 at 48.136 kb on - strand, within GFF3847 at 48.136 kb on - strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.841 kb on - strand, within GFF3847 at 48.841 kb on - strand, within GFF3847 at 48.841 kb on - strand, within GFF3847 at 48.980 kb on + strand at 48.980 kb on + strand at 48.980 kb on + strand at 48.980 kb on + strand at 48.981 kb on - strand at 48.981 kb on - strand at 48.981 kb on - strand at 48.981 kb on - strand at 48.981 kb on - strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.062 kb on - strand at 49.062 kb on - strand at 49.062 kb on - strand at 49.062 kb on - strand at 49.102 kb on + strand at 49.103 kb on - strand at 49.103 kb on - strand at 49.255 kb on + strand, within GFF3848 at 49.255 kb on + strand, within GFF3848 at 49.256 kb on - strand, within GFF3848 at 49.256 kb on - strand, within GFF3848 at 49.276 kb on + strand, within GFF3848 at 49.282 kb on + strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.455 kb on + strand, within GFF3848 at 49.456 kb on - strand, within GFF3848 at 49.456 kb on - strand, within GFF3848 at 49.456 kb on - strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.695 kb on + strand at 49.695 kb on + strand at 49.696 kb on - strand at 49.696 kb on - strand at 49.972 kb on + strand, within GFF3849 at 49.972 kb on + strand, within GFF3849 at 49.972 kb on + strand, within GFF3849 at 49.972 kb on + strand, within GFF3849
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 47,406 - -1.7 47,408 - +0.5 47,408 - +0.8 47,408 - -1.3 47,408 - -0.6 47,408 - -0.4 47,408 - -0.4 47,408 - +0.5 47,408 - -0.8 47,408 - -0.0 47,408 - +1.0 47,408 - -0.7 47,408 - -1.3 47,408 - +0.3 47,408 - -1.4 47,408 - -1.2 47,408 - -1.2 47,408 - +0.7 47,408 - +0.4 47,408 - -0.1 47,408 - +0.0 47,408 - -1.9 47,408 - -0.9 47,408 - +1.4 47,408 - -0.6 47,408 - -1.1 47,408 - -0.4 47,408 - +0.7 47,408 - +0.6 47,408 - +0.6 47,408 - -0.0 47,408 - +1.9 47,408 - -0.0 47,408 - -0.4 47,408 - +0.6 47,408 - +0.3 47,408 - -0.7 47,408 - +0.5 47,408 - -0.0 47,408 - +0.4 47,408 - +1.0 47,408 - +1.2 47,408 - +0.8 47,408 - -1.4 47,408 - +1.3 47,408 - +1.9 47,408 - +0.4 47,408 - +0.3 47,412 - +0.6 47,412 - +0.8 47,412 - -0.1 47,412 - +1.2 47,412 - -1.4 47,412 - -1.7 47,412 - +0.3 47,412 - +0.1 47,412 - -0.0 47,412 - +0.7 47,412 - +0.0 47,412 - +0.9 47,412 - +0.3 47,412 - -1.2 47,412 - -0.9 47,412 - -0.7 47,412 - +0.2 47,412 - -1.0 47,412 - +0.2 47,412 - +0.2 47,412 - +0.6 47,412 - -0.6 47,412 - +0.1 47,412 - +0.1 47,420 - +0.5 47,959 + -0.4 47,959 + +0.6 47,959 + -0.9 47,959 + -0.3 47,959 + +0.4 47,959 + -0.1 47,959 + -0.8 47,959 + -1.4 47,959 + -0.1 47,959 + +1.0 47,959 + -0.1 47,959 + +0.1 47,959 + +1.3 47,959 + -0.5 47,959 + +0.3 47,959 + -1.5 47,959 + -1.5 47,959 + +0.2 47,959 + +0.6 47,959 + -0.6 47,960 - +1.7 47,960 - -0.1 47,960 - -1.4 47,960 - -1.2 47,960 - +1.0 47,960 - -1.0 47,960 - -0.5 47,960 - -0.7 47,960 - -1.9 47,960 - -1.3 47,960 - -0.6 47,960 - -0.2 47,960 - +2.6 47,960 - -0.6 47,960 - -0.1 47,960 - -0.0 47,960 - -0.1 47,960 - -1.0 47,960 - -0.2 47,960 - +0.8 48,135 + GFF3847 0.18 -0.4 48,136 - GFF3847 0.18 -0.4 48,136 - GFF3847 0.18 +1.0 48,136 - GFF3847 0.18 -0.7 48,537 + GFF3847 0.54 +1.4 48,537 + GFF3847 0.54 +0.9 48,537 + GFF3847 0.54 -0.7 48,537 + GFF3847 0.54 -1.4 48,537 + GFF3847 0.54 -0.4 48,538 - GFF3847 0.54 -1.2 48,538 - GFF3847 0.54 -0.0 48,538 - GFF3847 0.54 +0.6 48,538 - GFF3847 0.54 +0.1 48,538 - GFF3847 0.54 -0.0 48,538 - GFF3847 0.54 -0.2 48,538 - GFF3847 0.54 -0.2 48,538 - GFF3847 0.54 -1.2 48,538 - GFF3847 0.54 -1.0 48,538 - GFF3847 0.54 -0.8 48,538 - GFF3847 0.54 -1.0 48,538 - GFF3847 0.54 -0.7 48,538 - GFF3847 0.54 -0.5 48,603 + GFF3847 0.60 -1.4 48,603 + GFF3847 0.60 +0.2 48,603 + GFF3847 0.60 +0.4 48,603 + GFF3847 0.60 -0.8 48,603 + GFF3847 0.60 +0.1 48,603 + GFF3847 0.60 -0.3 48,603 + GFF3847 0.60 -0.0 48,603 + GFF3847 0.60 -0.4 48,603 + GFF3847 0.60 -0.0 48,603 + GFF3847 0.60 -1.3 48,604 - GFF3847 0.60 -0.5 48,604 - GFF3847 0.60 -0.2 48,604 - GFF3847 0.60 -0.0 48,604 - GFF3847 0.60 -1.7 48,604 - GFF3847 0.60 -0.8 48,840 + GFF3847 0.81 -0.6 48,840 + GFF3847 0.81 -1.6 48,840 + GFF3847 0.81 -0.6 48,840 + GFF3847 0.81 +0.2 48,840 + GFF3847 0.81 -0.2 48,840 + GFF3847 0.81 -0.3 48,840 + GFF3847 0.81 -0.1 48,840 + GFF3847 0.81 -0.9 48,841 - GFF3847 0.81 -1.6 48,841 - GFF3847 0.81 -0.1 48,841 - GFF3847 0.81 -1.6 48,980 + -2.3 48,980 + -0.7 48,980 + -0.6 48,980 + -0.9 48,981 - -0.3 48,981 - +1.4 48,981 - +0.2 48,981 - -0.0 48,981 - -0.3 49,061 + +0.7 49,061 + +0.2 49,061 + +0.1 49,061 + -0.6 49,061 + -1.0 49,061 + -2.7 49,061 + -1.1 49,062 - +0.5 49,062 - -0.1 49,062 - +0.7 49,062 - -0.4 49,102 + -1.1 49,103 - +0.2 49,103 - -0.8 49,255 + GFF3848 0.32 +1.6 49,255 + GFF3848 0.32 -0.7 49,256 - GFF3848 0.32 +0.1 49,256 - GFF3848 0.32 -0.2 49,276 + GFF3848 0.35 -1.0 49,282 + GFF3848 0.36 +1.0 49,283 - GFF3848 0.36 +0.4 49,283 - GFF3848 0.36 -1.0 49,283 - GFF3848 0.36 +1.3 49,283 - GFF3848 0.36 +0.4 49,288 + GFF3848 0.37 -0.7 49,288 + GFF3848 0.37 +0.5 49,288 + GFF3848 0.37 +0.4 49,288 + GFF3848 0.37 -0.2 49,288 + GFF3848 0.37 -0.8 49,288 + GFF3848 0.37 -1.1 49,288 + GFF3848 0.37 -1.1 49,288 + GFF3848 0.37 -1.0 49,288 + GFF3848 0.37 -0.7 49,288 + GFF3848 0.37 +1.2 49,288 + GFF3848 0.37 +1.4 49,289 - GFF3848 0.37 +0.9 49,289 - GFF3848 0.37 -0.3 49,289 - GFF3848 0.37 +0.5 49,289 - GFF3848 0.37 -0.5 49,289 - GFF3848 0.37 -0.0 49,289 - GFF3848 0.37 -0.3 49,289 - GFF3848 0.37 -0.2 49,289 - GFF3848 0.37 -0.3 49,289 - GFF3848 0.37 -0.7 49,289 - GFF3848 0.37 -0.3 49,289 - GFF3848 0.37 -0.4 49,289 - GFF3848 0.37 -0.5 49,455 + GFF3848 0.62 -0.2 49,456 - GFF3848 0.62 +0.2 49,456 - GFF3848 0.62 -1.7 49,456 - GFF3848 0.62 +0.8 49,612 + GFF3848 0.85 -0.4 49,612 + GFF3848 0.85 -0.6 49,612 + GFF3848 0.85 +0.2 49,612 + GFF3848 0.85 -0.3 49,612 + GFF3848 0.85 -0.9 49,612 + GFF3848 0.85 -0.3 49,612 + GFF3848 0.85 -0.3 49,612 + GFF3848 0.85 -0.2 49,612 + GFF3848 0.85 -0.9 49,613 - GFF3848 0.85 +0.2 49,613 - GFF3848 0.85 -0.5 49,613 - GFF3848 0.85 -0.1 49,613 - GFF3848 0.85 -0.7 49,613 - GFF3848 0.85 +0.1 49,613 - GFF3848 0.85 -0.1 49,613 - GFF3848 0.85 -0.3 49,613 - GFF3848 0.85 -1.3 49,613 - GFF3848 0.85 -2.0 49,613 - GFF3848 0.85 -0.9 49,613 - GFF3848 0.85 -0.6 49,695 + +0.1 49,695 + -0.7 49,696 - -0.5 49,696 - -0.7 49,972 + GFF3849 0.65 -0.3 49,972 + GFF3849 0.65 +0.3 49,972 + GFF3849 0.65 -0.1 49,972 + GFF3849 0.65 -0.6
Or see this region's nucleotide sequence