Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3714 and GFF3715 are separated by 14 nucleotides GFF3715 and GFF3716 overlap by 4 nucleotides GFF3716 and GFF3717 are separated by 8 nucleotides GFF3717 and GFF3718 are separated by 20 nucleotides
GFF3714 - FMN reductase (NADH) RutF (EC 1.5.1.42), at 212,983 to 213,531
GFF3714
GFF3715 - Probable malonic semialdehyde reductase RutE (EC 1.1.1.298), at 213,546 to 214,145
GFF3715
GFF3716 - Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway, at 214,142 to 214,948
GFF3716
GFF3717 - Putative aminoacrylate peracid reductase RutC @ RidA/YER057c/UK114 superfamily protein, at 214,957 to 215,349
GFF3717
GFF3718 - Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (EC 3.5.1.110), at 215,370 to 216,161
GFF3718
Position (kb) 214
215 Strain fitness (log2 ratio) -2
-1
0
1 at 213.856 kb on + strand, within GFF3715 at 214.027 kb on + strand, within GFF3715 at 214.027 kb on + strand, within GFF3715 at 214.028 kb on - strand, within GFF3715 at 214.150 kb on + strand at 214.522 kb on - strand, within GFF3716 at 214.758 kb on + strand, within GFF3716 at 214.759 kb on - strand, within GFF3716 at 214.759 kb on - strand, within GFF3716 at 214.759 kb on - strand, within GFF3716 at 215.037 kb on - strand, within GFF3717 at 215.264 kb on + strand, within GFF3717 at 215.264 kb on + strand, within GFF3717 at 215.264 kb on + strand, within GFF3717 at 215.264 kb on + strand, within GFF3717 at 215.265 kb on - strand, within GFF3717 at 215.265 kb on - strand, within GFF3717 at 215.265 kb on - strand, within GFF3717 at 215.297 kb on + strand, within GFF3717 at 215.297 kb on + strand, within GFF3717 at 215.298 kb on - strand, within GFF3717 at 215.479 kb on + strand, within GFF3718 at 215.480 kb on - strand, within GFF3718 at 215.480 kb on - strand, within GFF3718 at 215.549 kb on - strand, within GFF3718 at 215.625 kb on + strand, within GFF3718 at 215.677 kb on + strand, within GFF3718 at 215.678 kb on - strand, within GFF3718 at 215.851 kb on + strand, within GFF3718 at 215.852 kb on - strand, within GFF3718 at 215.852 kb on - strand, within GFF3718
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 213,856 + GFF3715 0.52 +0.3 214,027 + GFF3715 0.80 -0.5 214,027 + GFF3715 0.80 -0.8 214,028 - GFF3715 0.80 -0.7 214,150 + -0.3 214,522 - GFF3716 0.47 -0.1 214,758 + GFF3716 0.76 +1.6 214,759 - GFF3716 0.76 -0.9 214,759 - GFF3716 0.76 -0.0 214,759 - GFF3716 0.76 +0.3 215,037 - GFF3717 0.20 -0.1 215,264 + GFF3717 0.78 +0.6 215,264 + GFF3717 0.78 +0.3 215,264 + GFF3717 0.78 +0.1 215,264 + GFF3717 0.78 -0.4 215,265 - GFF3717 0.78 -1.5 215,265 - GFF3717 0.78 +0.8 215,265 - GFF3717 0.78 -2.7 215,297 + GFF3717 0.87 -0.0 215,297 + GFF3717 0.87 -1.3 215,298 - GFF3717 0.87 -0.9 215,479 + GFF3718 0.14 -1.6 215,480 - GFF3718 0.14 -0.2 215,480 - GFF3718 0.14 -1.7 215,549 - GFF3718 0.23 -0.7 215,625 + GFF3718 0.32 -0.9 215,677 + GFF3718 0.39 -0.4 215,678 - GFF3718 0.39 +0.8 215,851 + GFF3718 0.61 -0.7 215,852 - GFF3718 0.61 +0.5 215,852 - GFF3718 0.61 +1.6
Or see this region's nucleotide sequence